CUDA Chemical Properties

Boiling point:

549.4±19.0 °C(Predicted)

Density 

1.03±0.1 g/cm3(Predicted)

storage temp. 

Store at -20°C

solubility 

≤1mg/ml in ethanol;5mg/ml in DMSO;10mg/ml in dimethyl formamide

form 

crystalline solid

pka

4.78±0.10(Predicted)

color 

White to off-white

CUDA Usage And Synthesis

Uses

CUDA is a potent inhibitor of soluble epoxide hydrolase (sEH), with IC50s of 11.1 nM and 112 nM for mouse sEH and human sEH, respectively[1]. CUDA selectively increases peroxisome proliferator-activated receptor (PPAR) alpha activity. CUDA may be valuable for the research of cardiovascular disease[2].

Definition

ChEBI: 12-[(Cyclohexylcarbamoyl)amino]dodecanoic acid is a medium-chain fatty acid.

Biological Activity

cuda is a soluble epoxide hydrolase (seh) inhibitor.epoxyeicosatrienoic acid metabolites of arachidonic acid, such as 11(12)-eet and 14(15)-eet, have been identified as endothelium derived hyperpolarizing factors with vasodilator activity. soluble epoxide hydrolase (seh) can catalyze the conversion of eets to the corresponding dihydroxy eicosatrienoic acids thereby diminishing their activity.

in vitro

in a previous study, in order to test if the cuda’s mechanism of action is retained upon structural modification, the dissociation constants of cuda was evaluated for mouse seh. results showed that for cuda, competitive a tight-binding inhibition kinetic was obtained with r2 > 0.99. moreover, the ki value of 3.8 nm was obtained for cuda. in addition, it was found that cuda was an inhibitor of seh exhibiting ic50 values of 11.1 nm and 112 nm for the mouse and human enzymes, respectively [1].

IC 50

11.1 and 112 nm for the mouse and human soluble epoxide hydrolase, respectively

References

[1] c. morisseau, m. h. goodrow, j. w. newman, et al. structural refinement of inhibitors of urea-based soluble epoxide hydrolases. biochemical pharmacology 63, 1599-1608 (2002).