Basic information Safety Supplier Related

5-Methyl-1H-pyrazole-3-carboxylic acid

Basic information Safety Supplier Related

5-Methyl-1H-pyrazole-3-carboxylic acid Basic information

Product Name:
5-Methyl-1H-pyrazole-3-carboxylic acid
Synonyms:
  • 5-METHYL-2H-PYRAZOLE-3-CARBOXYLIC ACID
  • 5-METHYLPYRAZOLE-3-CARBOXYLIC ACID
  • 5-Methyl-1H-pyrazole-3-carboxylic acid ,97%
  • U-19425
  • 3-Carboxy-5-methyl-1H-pyrazole
  • 5-Methyl-1H-pyrazole-3-ca...
  • IFLAB-BB F0917-7550
  • IFLAB-BB F2120-0002
CAS:
402-61-9
MF:
C5H6N2O2
MW:
126.11
EINECS:
673-162-9
Product Categories:
  • Building Blocks
  • Heterocyclic Building Blocks
  • Pyrazoles
  • Pyrazole
  • Heterocyclic Compounds
Mol File:
402-61-9.mol
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5-Methyl-1H-pyrazole-3-carboxylic acid Chemical Properties

Melting point:
241 °C
Boiling point:
388.8±22.0 °C(Predicted)
Density 
1.404±0.06 g/cm3(Predicted)
storage temp. 
Keep in dark place,Sealed in dry,Room Temperature
solubility 
Soluble in dimethyl sulfoxide.
form 
powder to crystal
pka
15.36±0.10(Predicted)
color 
White to Yellow to Orange
InChI
InChI=1S/C5H6N2O2/c1-3-2-4(5(8)9)7-6-3/h2H,1H3,(H,6,7)(H,8,9)
InChIKey
WSMQKESQZFQMFW-UHFFFAOYSA-N
SMILES
N1C(C)=CC(C(O)=O)=N1
CAS DataBase Reference
402-61-9(CAS DataBase Reference)
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Safety Information

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-37/39
WGK Germany 
3
RTECS 
UQ6407500
HazardClass 
IRRITANT
HS Code 
29331990

MSDS

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5-Methyl-1H-pyrazole-3-carboxylic acid Usage And Synthesis

Chemical Properties

White to light yellow solid

Uses

3-Methylpyrazole-5-carboxylic acid is a potent and selective D-amino acid oxidase (DAO) (also known as DAAO, DAMOX and OXDA) inhibitor that protects DAO cells from oxidative stress induced by D-Serine. 3-Methylpyrazole-5-carboxylic acid specifically prevents formalin-induced tonic pain.

Definition

ChEBI: A memebr of the class of pyrazoles that is 1H-pyrazole with methyl and carboxylic acid group substituents at positions 5 and 3 respectively.

General Description

3-Methylpyrazole-5-carboxylic acid is a potent and selective D-amino acid oxidase (DAO) (also known as DAAO, DAMOX and OXDA) inhibitor that protects DAO cells from oxidative stress induced by D-Serine. 3-Methylpyrazole-5-carboxylic acid specifically prevents formalin-induced tonic pain.

Synthesis

67-51-6

3112-31-0

402-61-9

General procedure for the synthesis of 3,5-pyrazoledicarboxylic acid and 5-methyl-1H-pyrazole-3-carboxylic acid from 3,5-dimethylpyrazole: 3,5-dimethyl-1H-pyrazole (78.5 g, 0.818 mol) was dissolved in 700 mL of water heated to 70 °C, potassium permanganate (517 g, 3.271 mol) was added slowly, and the temperature was kept no higher than 90 °C. After completion of the reaction, the mixture was cooled to room temperature, filtered to remove the resulting MnO2 precipitate, and washed with distilled water. The filtrate was acidified to pH 2 with dilute aqueous hydrochloric acid and allowed to stand overnight. The precipitate was collected by filtration and washed with distilled water to afford 3,5-pyrazoledicarboxylic acid (41.75 g, 33% yield) as white crystals with melting point 257-258 °C. 1H NMR (D2O, δ): 7.07 (s, 1H, 4-H). After isolation of 3,5-pyrazoledicarboxylic acid, the remaining aqueous phase filtrate was neutralized to pH 5-6, the precipitate was collected by filtration and washed with distilled water to afford 5-methyl-1H-pyrazole-3-carboxylic acid (18.1 g, 18% yield) as white crystals, melting point 210-211°C.1H NMR (D2O, δ): 2.25 (s, 3H, CH3), 6.42 (s, 1H, 4-H). 4-H).

in vivo

AS057278 (PO; 20 mg/kg b.i.d for 28 days; 80 mg/kg single dosage) normalizes?phencyclidine?(PCP)-induced?prepulse inhibition[1].
AS057278 (10 mg/kg; PO and IV; single dosage) exhibits good pharmacokinetic effects[1].
Pharmacokinetic Parameters of AS057278 in male Sprague-Dawley rats[1].

IV (10 mg/kg)PO (10 mg/kg)
C0 (ng/mL)100,557.3
Cmax (ng/mL)73,559.88088.8
tmax (h)0.0831
CZ (ng/mL)26.840.5
tZ (h)2424
AUCZ (ng/mL·h)45,596.218,254.4
λZ (h^-1)0.1240.096
AUC (ng/mL·h)45,810.918,649.9
VZ (L/kg)1.76
VSS (L/kg)0.24
CL (L/kg/h)0.22
MRT (h)1.087
F0.407
Animal Model:Males C57BL/6J mice[1]
Dosage:80 mg/kg, 20 mg/kg
Administration:PO; 20 mg/kg b.i.d for 28 days; 80 mg/kg single dosage
Result:Normalized?phencyclidine?(PCP)-induced?prepulse inhibition?after acute (80?mg/kg) and chronic (20?mg/kg b.i.d.)?oral administration?in mice.
Animal Model:Male Sprague-Dawley rats[1]
Dosage:10 mg/kg
Administration:PO and IV; single dosage (Pharmacokinetics Analysis)
Result:Exhibited good pharmacokinetic effect.

References

[1] Russian Journal of Organic Chemistry, 2016, vol. 52, # 9, p. 1322 - 1325
[2] Zh. Org. Khim., 2016, vol. 52, # 9, p. 1334 - 1337,4

5-Methyl-1H-pyrazole-3-carboxylic acid Preparation Products And Raw materials

Raw materials

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