BTO-1 Chemical Properties

Boiling point:

553.0±60.0 °C(Predicted)

Density 

1.89±0.1 g/cm3(Predicted)

storage temp. 

2-8°C

solubility 

H2O: <2ML/mL

form 

solid

pka

14.67±0.30(Predicted)

color 

tan

Safety Information

Hazard Codes 

Xn

Risk Statements 

20/21/22-36/37/38

Safety Statements 

26-36/37

WGK Germany 

3

BTO-1 Usage And Synthesis

Uses

BTO-1 is a benzothiazolo-N-oxide Plk1 inhibitor.

General Description

A cell-permeable benzothiazolo-N-oxide compound that targets the ATP-binding pocket of polo-like kinase and is shown to inhibit Plk1 kinase activity in cell-free kinase assays (IC₅₀ = 8.0 μM) and suppress the phosphorylation of cellular Plk1 substrate Cdc25C in rat kangaroo kidney-derived PTK cells (75% inhibition at 6.3 μM). BTO-1 treatment of cultured cells results in mitosis defects consistent with loss-of-Plk1 phenotypes seen in Plk1 RNAi-treated U20S cells, including the appearance of monopolar spindles and the reduction of γ-tubulin at the centrosomes. Plk1 inhibition by BTO-1 in HeLa cells results in a blockage of Rho and Rho-GEF recruitment, which is essential for the assembly of a functional contractile ring.

Biochem/physiol Actions

BTO-1 is a polo-like kinase (Plk) inhibitor.