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MonoMethyl auristatin E

Product Name
MonoMethyl auristatin E
CAS No.
474645-27-7
Chemical Name
MonoMethyl auristatin E
Synonyms
MMAE;Vedotin;N-Methyl-L-valyl-N-[(1S,2R)-4-[(2S)-2-[(1R,2R)-3-[[(1R,2S)-2-hydroxy-1-methyl-2-phenylethyl]amino]-1-methoxy-2-methyl-3-oxopropyl]-1-pyrrolidinyl]-2-methoxy-1-[(1S)-1-methylpropyl]-4-oxobutyl]-N-methyl-L-valinamide Monomethyl;MonoMethyl auristatin E (MMAE);(S)-N-((3R,4S,5S)-1-((S)-2-((1R,2R)-3-(((1R,2R)-1-Hydroxy-1-phenylpropan-2-yl)amino)-1-methoxy-2-methyl-3-oxopropyl)pyrrolidin-1-yl)-3-methoxy-5-methyl-1-oxohe;N-Methyl-L-valyl-N-[(1S,2R)-4-[(2S)-2-[(1R,2R)-3-[[(1R,2S)-2-hydroxy-1-methyl-2-phenylethyl]amino]-1-methoxy-2-methyl-3-oxopropyl]-1-pyrrolidinyl]-2-methoxy-1-[(1S)-1-methylpropyl]-4-oxobutyl]-N-methyl-L-valinamide;CS-835;SGD-1010;HM-297C-22;[3H]-Vedotin
CBNumber
CB02624235
Molecular Formula
C39H67N5O7
Formula Weight
717.98
MOL File
474645-27-7.mol
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MonoMethyl auristatin E Property

Melting point:
>90°C (dec.)
Boiling point:
873.5±65.0 °C(Predicted)
Density 
1.088±0.06 g/cm3(Predicted)
storage temp. 
-20°C Freezer
solubility 
DMSO (Slightly), Methanol (Slightly, Sonicated)
pka
13.66±0.20(Predicted)
form 
Solid
color 
White to Off-White
InChI
InChI=1S/C39H67N5O7/c1-13-25(6)34(43(10)33(24(4)5)39(49)42-38(48)32(40-9)23(2)3)30(50-11)22-31(45)44-21-17-20-29(44)36(51-12)26(7)37(47)41-27(8)35(46)28-18-15-14-16-19-28/h14-16,18-19,23-27,29-30,32-36,40,46H,13,17,20-22H2,1-12H3,(H,41,47)(H,42,48,49)/t25-,26+,27+,29-,30+,32-,33-,34-,35+,36+/m0/s1
InChIKey
RJACXSRFJLSJEZ-UIJRFTGLSA-N
SMILES
C(NC(=O)[C@H](C(C)C)NC)(=O)[C@H](C(C)C)N([C@@H]([C@@H](C)CC)[C@H](OC)CC(N1CCC[C@H]1[C@H](OC)[C@@H](C)C(N[C@H](C)[C@@H](O)C1=CC=CC=C1)=O)=O)C
CAS DataBase Reference
474645-27-7
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Cayman Chemical
Product number
16267
Product name
Monomethyl Auristatin E
Purity
≥95%
Packaging
500μg
Price
$81
Updated
2024/03/01
Cayman Chemical
Product number
16267
Product name
Monomethyl Auristatin E
Purity
≥95%
Packaging
1mg
Price
$152
Updated
2024/03/01
Cayman Chemical
Product number
16267
Product name
Monomethyl Auristatin E
Purity
≥95%
Packaging
5mg
Price
$356
Updated
2024/03/01
Cayman Chemical
Product number
16267
Product name
Monomethyl Auristatin E
Purity
≥95%
Packaging
10mg
Price
$553
Updated
2024/03/01
TRC
Product number
M425410
Product name
MMAE
Packaging
100mg
Price
$910
Updated
2021/12/16
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MonoMethyl auristatin E Chemical Properties,Usage,Production

Description

MMAE is a synthetic antineoplastic agent. Because of its toxicity, it cannot be used as a drug itself; instead, it is linked to a monoclonal antibody (MAB) which directs it to the cancer cells. Monomethyl auristatin E or MMAE is 100-1000 times more potent than doxorubicin. It is a very potent antimitotic agent that inhibits cell division by blocking the polymerization of tubulin.

Chemical Properties

Monomethyl auristatin E (MMAE) is a synthetic antineoplastic agent. Because of its toxicity, it cannot be used as a drug itself; instead, it is linked to a monoclonal antibody (MAB) which directs it to the cancer cells. In International Nonproprietary Names for MMAE-MAB-conjugates, the name vedotin refers to MMAE plus its linking structure to the antibody. It is a potent antimitotic drug derived from peptides occurring in marine shell-less mollusc Dolabella auricularia called dolastatins which show potent activity in preclinical studies, both in vitro and in vivo, against a range of lymphomas, leukemia and solid tumors. These drugs show potency of up to 200 times that of vinblastine, another antimitotic drug used for Hodgkin lymphoma as well as other types of cancer.
MMAE is actually desmethyl-auristatin E; that is, the N-terminal amino group has only one methyl substituent instead of two as in auristatin E itself.

Uses

MMAE is an effective and synthetic cytotoxic analog of Dolastatin 10 which is a potent antimitotic polypeptide isolated from a marine animal.

Uses

Dolastatin 10 is a natural antimitotic and antineoplastic agent that binds to tubulin and inhibits tubulin polymerization. Monomethyl Auristatin E (MMAE) is a synthetic analog of dolastatin 10 that similarly inhibits tubulin polymerization and exhibits potent cytotoxicity. It is commonly conjugated with monoclonal antibodies directed at antigens specific to cancer cells for tumor-directed cytotoxicity. MMAE is typically coupled to the antibody via a protease-cleavable linker, allowing separation of the drug from the antibody following intracellular localization.[Cayman Chemical]

Biological Activity

monomethyl auristatin e(mmae) is a potent antimitotic agent by blocking the polymerisation of tubulin.microtubules play essential role in the function of the cell. microtubules are also reported to be involved in migration, transport and reorganization and have numerous dynamic roles including movement through motor proteins such as dynein and kinesin and the separation and segregation ofchromosomes during cell division.

Biochem/physiol Actions

Monomethyl Auristatin E (MMAE) is a highly potent peptidyl antimitotic agent that blocks the polymerization of tubulin. Monomethyl Auristatin E is a component of a clinically approved antibodydirected conjugates Brentuximab vedotin, Glembatumumab vedotin, Enfortumab vedotin and Polatuzumab vedotin.

in vitro

the cytotoxic effects of the mmae conjugates on h3396 cells were determined using both pulsed and long-term drug exposure assays. it was found that under both exposure conditions, high degrees of immunological specificity were obtained with the val-cit conjugates. cbr96-val-cit-mmae was highly active at <1/100th of the concentration required for antigen saturation [1].

in vivo

in vivo therapy tests were undertaken in athymic mice with subcutaneous l2987 human lung adenocarcinoma xenografts. mmae conjugates were administered at 3 mg mab component/kg/dose. all of the tested mmae conjugates were highly efficacious, leading to long-term regressions of established tumors, whereas the nonbinding control conjugates had no effect on tumor growth. in addition, there were no apparent toxicities associated with conjugate treatment [1].

IC 50

less than 1 nm for various cancer cell lines

References

[1] doronina so,toki be,torgov my,mendelsohn ba,cerveny cg,chace df,deblanc rl,gearing rp,bovee td,siegall cb,francisco ja,wahl af,meyer dl,senter pd. development of potent monoclonal antibody auristatin conjugates for cancer therapy. nat biotechnol.2003 jul;21(7):778-84.
[2] jian xu*, priya agarwal, ola saad, et al. clinical pharmacokinetics (pk) of anti-muc16 antibody-drug conjugates (adcs), dmuc5754a, in patients with platinum-resistant ovarian cancer: results from phase i study. this poster was presented at world adc summit 2014.

MonoMethyl auristatin E Preparation Products And Raw materials

Raw materials

Preparation Products

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MonoMethyl auristatin E Suppliers

Zhejiang Huida Biotech Co., LTD
Tel
0571-89903882 13626641628
Email
jiangnan@huidabiotech.com
Country
China
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Shanghai Send Pharmaceutical Technology Co., Ltd.
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+86-021-50266790 Q2816483246
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021-50266790
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sale@shsendpharm.com
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China
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Shanghai Linka Biotechnology Co., Ltd.
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15901858719
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qq2126935757
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2126935757@qq.com
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China
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Tianjin Chempharmatech Co.,Ltd.
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022-66211079 13212288319
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qq1405432731
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sales@chempharmatech.com
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China
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Shanghai ruichang pharmaceutical technology co., LTD
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18621054990
Email
maychemicals@sina.com
Country
China
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Abydos Scientific
Tel
025-84767922 18936879710
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sales@abydoscientific.com
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China
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Shanghai Haoyuan Chemexpress Co., Ltd.
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021-58950125
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021-58955996
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info@chemexpress.com
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China
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ACROBiosystems
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18514007688
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jiaxin.zhao@acrobiosystems.com
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China
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EnliPharma Technology Co., Ltd
Tel
0551-66399836 18955197623
Email
sales@enlipharma.com
Country
China
ProdList
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YJ Pharma
Tel
13962794339 13962794339
Email
3686954964@qq.com
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China
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View Lastest Price from MonoMethyl auristatin E manufacturers

Anhui Ruihan Technology Co., Ltd
Product
MonoMethyl auristatin E 474645-27-7
Price
US $30.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
1000t/year
Release date
2023-09-07
Hebei Mojin Biotechnology Co., Ltd
Product
MonoMethyl auristatin E 474645-27-7
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
50000KG/month
Release date
2023-05-19
Zhuozhou Wenxi import and Export Co., Ltd
Product
MMAE;[3H]-Vedotin 474645-27-7
Price
US $15.00-10.00/KG
Min. Order
1KG
Purity
99%+ HPLC
Supply Ability
Monthly supply of 1 ton
Release date
2021-07-11

474645-27-7, MonoMethyl auristatin ERelated Search:


  • MonoMethyl auristatin E(MMAE, vedotin)
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  • Monomethyl auristatin E, >=98%
  • (S)-N-((3R,4S,5S)-1-((S)-2-((1R,2R)-3-(((1R,2R)-1-Hydroxy-1-phenylpropan-2-yl)amino)-1-methoxy-2-methyl-3-oxopropyl)pyrrolidin-1-yl)-3-methoxy-5-methyl-1-oxohe
  • MonoMethyl auristatin E (MMAE)
  • N-Methyl-L-valyl-N-[(1S,2R)-4-[(2S)-2-[(1R,2R)-3-[[(1R,2S)-2-hydroxy-1-methyl-2-phenylethyl]amino]-1-methoxy-2-methyl-3-oxopropyl]-1-pyrrolidinyl]-2-methoxy-1-[(1S)-1-methylpropyl]-4-oxobutyl]-N-methyl-L-valinamide
  • MMAE(Monomethyl auristatin E)
  • (2S)-N-[(2S)-1-[[(3R,4S,5S)-1-[(2S)-2-[(1R,2R)-3-[[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino]-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl]-3-methoxy-5-methyl-1-oxoheptan-4-yl]-methylamino]-3-methyl-1-oxobutan-2-yl]-3-methyl-2-(methylamino)butanamide
  • [3H]-Vedotin
  • (S)-N-((3R,4S,5S)-1-((S)-2-((1R,2R)-3-(((1R,2R)-1-Hydroxy-1-phenylpropan-2-yl)amino)-1-methoxy
  • best price 99% Monomethyl Auristatin E, MMAE factory in stock CAS NO.474645-27-7
  • CS-835
  • MMAE; VEDOTIN;?MONOMETHYL AURISTATIN E
  • L-Valinamide, N-methyl-L-valyl-N-[(1S,2R)-4-[(2S)-2-[(1R,2R)-3-[[(1R,2S)-2-hydroxy-1-methyl-2-phenylethyl]amino]-1-methoxy-2-methyl-3-oxopropyl]-1-pyrrolidinyl]-2-methoxy-1-[(1S)-1-methylpropyl]-4-oxobutyl]-N-methyl-
  • Monomethyl auristatin E (vedotin)
  • L-Valinamide, N-methyl-L-valyl-N-[(1S,2R)-4-[(2S)-2-[(1R,2R)-3-[[(1R,2S)-2-hydroxy-1-methyl-2-phenylethyl]amino]-1-methoxy-2-methyl-3-oxopropyl]-1-pyrrolidinyl]-2-methoxy-1-[(1S)-1-methylpropyl]-4-oxob
  • Ethanol,2,2'-[1,5-naphthalenediylbis(oxy-2,3-ethanediyloxy)]bis-
  • (2S)-N-[(2S)-1-[[(3R,5S)-1-[(2S)-2-[(1R,2R)-3-[[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino]-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl]-3-methoxy-5-methyl-1-oxoheptan-4-yl]-methylamino]-3-methyl-1-oxobutan-2-yl]-3-methyl-2-(methylamino)butanamide
  • N-Methyl-L Valyl-N - [(1S, 2R) -4- [(2S) -2- [(1R, 2R) -3- [(1R, 2S) -2-hydroxy-1-methyl-2-phenylethyl] amino] -1-methoxy-2-methyl-3-oxopropyl] -1-pyrrolidine] -2-methoxy-1- [(1S) -1-methylpropyl] -4-oxobutyl] - N-methyl-L Valamide
  • SGD-1010
  • 474645-27-7
  • 474645-27-8
  • 74645-27-7
  • C39H67N5O7
  • intermediates
  • Pharmaceutical
  • ADCs