4-(4-(DIMETHYLAMINO)STYRYL)-N-METHYLPYRIDINIUM IODIDE

4-(4-(DIMETHYLAMINO)STYRYL)-N-METHYLPYRIDINIUM IODIDE Property

Melting point:

261–262℃

solubility 

Soluble in methanol, N,N-dimethylformamide

form 

Solid

color 

Greenish purple solid;2 Purple solid; Red needles

Appearance

Solid Powder

λmax

475 nm

Major Application

Electrochromic materials;glass;nonlinear optical (NLO) materials;photoconductors;thin films

Biological Applications

Detecting nucleic acids,prostate cancer; treating amyloidosis disorders,bacterial infection

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P321Specific treatment (see … on this label).

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P362Take off contaminated clothing and wash before reuse.

4-(4-(DIMETHYLAMINO)STYRYL)-N-METHYLPYRIDINIUM IODIDE Chemical Properties,Usage,Production

Description

4-(4-(dimethylamino)styryl)-n-methylpyridinium iodide is utilized in the biomedical industry as a fluorescent probe that specifically stains mitochondria. It is commonly used to study mitochondrial function, localization, and dynamics in various cell types. Additionally, this compound aids in investigating diseases associated with mitochondrial dysfunction such as neurodegenerative disorders and cancer.

Uses

4-Di-1-ASP is a styryl dye used to stain glioma cells in living brain tissue for analysis of cell structure, viability, proliferation and endocytosis, cytokinesis and phagocytosis, as well as for observation of mitochondrial structures in living cells. 4-Di-1-ASP fluoresces green when imaged microscopically (λex /λem = 475/606 nm) [1][2].

Definition

ChEBI: 4-[4-(dimethylamino)styryl]-N-methylpyridinium iodide is an organic iodide salt consisting of pyridinium iodide having a methyl substituent at the 1-position and a 4-dimethylaminostyryl substituent at the 4-position. It has a role as a fluorochrome. It is an organic iodide salt and a pyridinium salt. It contains a 4-[4-(dimethylamino)styryl]-N-methylpyridinium.

References

[1] Lilia Y Kucheryavykh, et al. Visualization of implanted GL261 glioma cells in living mouse brain slices using fluorescent 4-(4-(dimethylamino)-styryl)-N-methylpyridinium iodide (ASP+). Biotechniques. 2012 Nov;53(5):305-9. DOI:10.2144/000113940
[2] Jensen J H Wong, et al. Simultaneous High-Throughput Conformational and Colloidal Stability Screening Using a Fluorescent Molecular Rotor Dye, 4-(4-(Dimethylamino)styryl)-N-Methylpyridinium Iodide (DASPMI). J Biomol Screen. 2016 Sep;21(8):842-50. DOI:10.1177/1087057116646553

Excitation / Emission Maximum

340 nm/488 nm