4',6-Diamidino-2-phenylindole dihydrochloride
- Product Name
- 4',6-Diamidino-2-phenylindole dihydrochloride
- CAS No.
- 28718-90-3
- Chemical Name
- 4',6-Diamidino-2-phenylindole dihydrochloride
- Synonyms
- DAPI;DAPI 2HCL;DAPI Stain;-Cellstain-Dapi;DAPI(meilunbio);DAPI Stain Solution;4',6-Diamidino-2-phe;DAPI (hydrochloride);DAPI, DIHYDROCHLORIDE;DAPI Staining Solution
- CBNumber
- CB1453377
- Molecular Formula
- C16H16ClN5
- Formula Weight
- 313.79
- MOL File
- 28718-90-3.mol
4',6-Diamidino-2-phenylindole dihydrochloride Property
- Melting point:
- >300°C (dec.)
- storage temp.
- 2-8°C
- solubility
- H2O: 10 mg/mL heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.
- form
- powder
- color
- Clear
- PH
- 4.0-5.0 (10g/l, H2O, 20℃)
- λmax
- 358 nm, 342 nm
- BRN
- 4894417
- Stability:
- PROTECT FROM LIGHT! Stable for 2 years as supplied. Solutions in DMSO may be aliquoted and stored at -20°C for up to 3 months.
- Biological Applications
- Nuclear apoptosis assay; targeting drug to cerebral neuron; treating amyloidosis,neurodegenerative diseases,Alzheimer’s disease,Down’s syndrome,type II diabetes; monitoring bioaerosols
- InChIKey
- FPNZBYLXNYPRLR-UHFFFAOYSA-N
- CAS DataBase Reference
- 28718-90-3(CAS DataBase Reference)
Safety
- Hazard Codes
- Xi,Xn
- Risk Statements
- 36/37/38-20/21/22
- Safety Statements
- 26-36-24/25-22-37/39
- WGK Germany
- 3
- RTECS
- NL5995050
- F
- 3-8-10-21
- HS Code
- 29339900
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H317May cause an allergic skin reaction
H335May cause respiratory irritation
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P271Use only outdoors or in a well-ventilated area.
P272Contaminated work clothing should not be allowed out of the workplace.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352IF ON SKIN: wash with plenty of soap and water.
N-Bromosuccinimide Price
- Product number
- MBD0020
- Product name
- DAPI ready made solution with Antifade
- Purity
- For Nuclear counterstain in Fluorescence Microscopy
- Packaging
- 10ML
- Price
- $127
- Updated
- 2024/03/01
- Product number
- 5.08741
- Product name
- DAPI Stain - CAS 28718-90-3 - Calbiochem
- Purity
- A 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl in H?O.
- Packaging
- 5MG
- Price
- $132
- Updated
- 2024/03/01
- Product number
- 32670
- Product name
- 4′,6-Diamidino-2-phenylindole dihydrochloride
- Purity
- BioReagent, suitable for fluorescence, ≥95.0% (HPLC)
- Packaging
- 5mg
- Price
- $122
- Updated
- 2024/03/01
- Product number
- 32670
- Product name
- 4′,6-Diamidino-2-phenylindole dihydrochloride
- Purity
- BioReagent, suitable for fluorescence, ≥95.0% (HPLC)
- Packaging
- 25mg
- Price
- $501
- Updated
- 2024/03/01
- Product number
- 268298
- Product name
- DAPI, Dihydrochloride
- Purity
- Cell-permeable DNA-binding dye.
- Packaging
- 10mg
- Price
- $224
- Updated
- 2024/03/01
4',6-Diamidino-2-phenylindole dihydrochloride Chemical Properties,Usage,Production
Description
DAPI is a fluorescent probe which is commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. It forms a fluorescent complex by attaching in the minor groove of A-
Chemical Properties
Yellow Solid
Uses
DAPI is used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex and as a cell permeable fluorescent minor groove-binding probe for DNA. For staining DNA in agarose gels
Uses
Suitable for
- DNA staining in agarose gels
- analysis of changes in DNA during apoptosis
- detection of mycoplasma
- photofootprinting of DNA
- immunofluorescent staining of cells
DAPI has been used:-
- in rapid monitoring of microbial contamination
- in chromosomal banding technique
- in detection of apoptotic cells
- in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA
- to stain mature pollen grains(0.5 mg/ml)
Uses
4',6-Diamidino-2-phenylindole dihydrochloride is used as a cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine.It is used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes.
General Description
DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.
Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
storage
-20°C
References
[1] W. C. RUSSELL D. H W CAROL NEWMAN. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses[J]. Nature, 1975, 253 5491: 461-462. DOI:10.1038/253461a0.
[2] BETTY I. TARNOWSKI James H N F Spinale. DAPI as a useful stain for nuclear quantitation.[J]. Biotechnic & Histochemistry, 1991, 22 1: 297-302. DOI:10.3109/10520299109109990.
[3] ANA KATRINA ESTANDARTE. The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes.[J]. Scientific Reports, 2016: 31417. DOI:10.1038/srep31417.
4',6-Diamidino-2-phenylindole dihydrochloride Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from 4',6-Diamidino-2-phenylindole dihydrochloride manufacturers
- Product
- 4',6-Diamidino-2-phenylindole dihydrochloride 28718-90-3
- Price
- US $0.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 50000KG/month
- Release date
- 2023-09-28
- Product
- 4',6-Diamidino-2-phenylindole dihydrochloride 28718-90-3
- Price
- US $1.00/KG
- Min. Order
- 1KG
- Purity
- 98%
- Supply Ability
- 1
- Release date
- 2018-08-06