Benzamidine hydrochloride
- Product Name
- Benzamidine hydrochloride
- CAS No.
- 1670-14-0
- Chemical Name
- Benzamidine hydrochloride
- Synonyms
- BenziMidaMide hydrochloride;BENZAMIDINE HCL;benzamidine hydroxychloride;s-dimethylester;BENZAMIDE HCL H2O;Benzamidine hydrochl;benzamidiniumchloride;BENZAMIDINE HYDROCHLORIDE;Benzamidin Hydrochloride;amidinobenzenehydrochloride
- CBNumber
- CB6100733
- Molecular Formula
- C7H9ClN2
- Formula Weight
- 156.61
- MOL File
- 1670-14-0.mol
Benzamidine hydrochloride Property
- Melting point:
- 86-88 °C(lit.)
- storage temp.
- 2-8°C
- solubility
- DMF: 25 mg/ml
DMSO: 25 mg/ml
Ethanol: 10 mg/ml
PBS (pH 7.2): 3 mg/ml - form
- Powder
- color
- White to off-white
- PH
- 4.0 to 6.5(50g/L, 25 ℃)
- Water Solubility
- soluble
- BRN
- 3594959
- Stability:
- Stable for 2 years from date of purchase as supplied. Solutions are not stable and must be prepared fresh daily
- InChIKey
- LZCZIHQBSCVGRD-UHFFFAOYSA-N
- CAS DataBase Reference
- 1670-14-0(CAS DataBase Reference)
- EPA Substance Registry System
- Benzenecarboximidamide, monohydrochloride (1670-14-0)
Safety
- Hazard Codes
- Xi
- Risk Statements
- 36/37/38
- Safety Statements
- 26-37/39-36
- WGK Germany
- 3
- RTECS
- CV6260000
- F
- 3-21
- HS Code
- 29252900
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
- Precautionary statements
-
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P280Wear protective gloves/protective clothing/eye protection/face protection.
N-Bromosuccinimide Price
- Product number
- 63226
- Product name
- Benzamidine hydrochloride 1 M solution
- Packaging
- 1mL
- Price
- $73.5
- Updated
- 2024/03/01
- Product number
- 199001-M
- Product name
- Benzamidine, Hydrochloride
- Purity
- Inhibitor of trypsin and trypsin-like enzymes.
- Packaging
- 25gm-m
- Price
- $101
- Updated
- 2021/12/16
- Product number
- 199001-M
- Product name
- Benzamidine, Hydrochloride - CAS 1670-14-0 - Calbiochem
- Purity
- Inhibitor of trypsin and trypsin-like enzymes.
- Packaging
- 25g
- Price
- $101
- Updated
- 2021/12/16
- Product number
- 199001-M
- Product name
- Benzamidine, Hydrochloride
- Purity
- Inhibitor of trypsin and trypsin-like enzymes.
- Packaging
- 5gm-m
- Price
- $55.7
- Updated
- 2021/12/16
- Product number
- 199001-M
- Product name
- Benzamidine, Hydrochloride - CAS 1670-14-0 - Calbiochem
- Purity
- Inhibitor of trypsin and trypsin-like enzymes.
- Packaging
- 5G
- Price
- $55.7
- Updated
- 2021/12/16
Benzamidine hydrochloride Chemical Properties,Usage,Production
Description
Benzamidine hydrochloride is a reversible inhibitor of trypsin, trypsin-like enzymes, and serine proteases. A concentration of approximately 1 mM is used for general protease inhibition. To inhibit proteases from yeast, a range of 0.5 to 4.0 mM is used and it is for the most part interchangeable with pepstatin A.
In addition to being a strong competitive inhibitor of trypsin, benzamidine HCl has been also shown to be a strong competitive inhibitor of thrombin and plasmin. It was also found to be as effective as aprotinin in the prevention of glucagon degradation in human plasma.
Chemical Properties
white to off-white powder
Uses
Benzamidine is a reversible inhibitor of serine proteases, including trypsin, plasmin, and thrombin (Kis = 35, 350, and 220 μM, respectively). In addition to its use as a general serine protease inhibitor, benzamidine is used, when immobilized, to purify novel proteases.
Preparation
To a stainless steel rocking autoclave equipped with a stirrer is added 103.0 gm (1.0 mole) of benzonitrile, 214.0 gm (4.0 mole) of ammonium chloride, and 306.0 gm (18.0 mole) of ammonia is introduced by means of a transfer bomb. The reaction mixture is heated at 150°C for 18 hr (pressure: 1300-6500 psig), cooled, and, with appropriate precautions for the safe control of the excess ammonia, is vented to atmospheric pressure. The reaction mixture is extracted with ether to remove approximately 5% unreacted benzonitrile, and then extracted with hot acetonitrile or ethanol to separate the amidine hydrochloride from the unreacted ammonium chloride. Concentration of the latter affords 120.5 gm (77%) of benzamidine hydrochloride, m.p. 161-163°C.
Biological Activity
Benzamidine hydrochloride is an reversible competitive inhibitor of trypsin-like serine proteases, with Kis of 97 μM, 21 μM, 20 μM and 110 μM for uPA, trypsin, tryptase and factor Xa, respectively.
Safety Profile
Moderately toxic byintraperitoneal route. When heated to decomposition itemits toxic vapors of NOx, HCl, and Cl-.
in vitro
Benzamidine hydrochloride has weak inhibition for tPA and thrombin, with Kis of 750 μM and 320 μM, respectively.
References
1) Markwardt?et al. (1968)?Comparative studies on the inhibition of trypsin, plasmin, and thrombin by derivatives of benzylamine and benzamide; Eur. J. Biochem.,?6?502 2) Deutscher?et al.?(1990),?Maintaining protein stability; Methods Enzymol.,?182?83 3) Jaswinski?et al.?(1990),?Preparations of extracts from yeast; Methods Enzymol.,?182?154 4) Ensinck?et al.?(1972),?Use of Benzamidine as a proteolytic inhibitor in the radioimmunoassay of glucagon in plasma; J. Clin. Endocrinol. Metab.,?35?463 5) Jeffcoate?et al. (1974),?Use of benzamidine to prevent the destruction of thyrotropin-releasing hormone (TRH) by blood; Endocrinol. Metab,?38?155
Benzamidine hydrochloride Preparation Products And Raw materials
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View Lastest Price from Benzamidine hydrochloride manufacturers
- Product
- Benzamidine hydrochloride 1670-14-0
- Price
- US $0.00-0.00/kg
- Min. Order
- 1kg
- Purity
- 98%
- Supply Ability
- 1Ton
- Release date
- 2022-09-16
- Product
- Benzamidine hydrochloride 1670-14-0
- Price
- US $15.00-10.00/KG
- Min. Order
- 1KG
- Purity
- 99%+ HPLC
- Supply Ability
- Monthly supply of 1 ton
- Release date
- 2021-07-10
- Product
- Benzamidine hydrochloride 1670-14-0
- Price
- US $15.00-10.00/KG
- Min. Order
- 1KG
- Purity
- 99%+ HPLC
- Supply Ability
- Monthly supply of 1 ton
- Release date
- 2021-07-09