ChemicalBook > CAS DataBase List > 4',6-Diamidino-2-phenylindole dihydrochloride

4',6-Diamidino-2-phenylindole dihydrochloride

Product Name
4',6-Diamidino-2-phenylindole dihydrochloride
CAS No.
28718-90-3
Chemical Name
4',6-Diamidino-2-phenylindole dihydrochloride
Synonyms
DAPI;DAPI 2HCL;DAPI Stain;-Cellstain-Dapi;DAPI(meilunbio);DAPI Stain Solution;4',6-Diamidino-2-phe;DAPI (hydrochloride);DAPI, DIHYDROCHLORIDE;DAPI Staining Solution
CBNumber
CB1453377
Molecular Formula
C16H16ClN5
Formula Weight
313.79
MOL File
28718-90-3.mol
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4',6-Diamidino-2-phenylindole dihydrochloride Property

Melting point:
>300°C (dec.)
storage temp. 
2-8°C
solubility 
H2O: 10 mg/mL heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.
form 
powder
color 
Clear
PH
4.0-5.0 (10g/l, H2O, 20℃)
λmax
358 nm, 342 nm
BRN 
4894417
Stability:
PROTECT FROM LIGHT! Stable for 2 years as supplied. Solutions in DMSO may be aliquoted and stored at -20°C for up to 3 months.
Biological Applications
Nuclear apoptosis assay; targeting drug to cerebral neuron; treating amyloidosis,neurodegenerative diseases,Alzheimer’s disease,Down’s syndrome,type II diabetes; monitoring bioaerosols
InChIKey
FPNZBYLXNYPRLR-UHFFFAOYSA-N
CAS DataBase Reference
28718-90-3(CAS DataBase Reference)
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Safety

Hazard Codes 
Xi,Xn
Risk Statements 
36/37/38-20/21/22
Safety Statements 
26-36-24/25-22-37/39
WGK Germany 
3
RTECS 
NL5995050
F 
3-8-10-21
HS Code 
29339900
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H317May cause an allergic skin reaction

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P271Use only outdoors or in a well-ventilated area.

P272Contaminated work clothing should not be allowed out of the workplace.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
MBD0020
Product name
DAPI ready made solution with Antifade
Purity
For Nuclear counterstain in Fluorescence Microscopy
Packaging
10ML
Price
$127
Updated
2024/03/01
Sigma-Aldrich
Product number
5.08741
Product name
DAPI Stain - CAS 28718-90-3 - Calbiochem
Purity
A 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl in H?O.
Packaging
5MG
Price
$132
Updated
2024/03/01
Sigma-Aldrich
Product number
32670
Product name
4′,6-Diamidino-2-phenylindole dihydrochloride
Purity
BioReagent, suitable for fluorescence, ≥95.0% (HPLC)
Packaging
5mg
Price
$122
Updated
2024/03/01
Sigma-Aldrich
Product number
32670
Product name
4′,6-Diamidino-2-phenylindole dihydrochloride
Purity
BioReagent, suitable for fluorescence, ≥95.0% (HPLC)
Packaging
25mg
Price
$501
Updated
2024/03/01
Sigma-Aldrich
Product number
268298
Product name
DAPI, Dihydrochloride
Purity
Cell-permeable DNA-binding dye.
Packaging
10mg
Price
$224
Updated
2024/03/01
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4',6-Diamidino-2-phenylindole dihydrochloride Chemical Properties,Usage,Production

Description

DAPI is a fluorescent probe which is commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. It forms a fluorescent complex by attaching in the minor groove of A-T rich sequences of DNA. DAPI is often used as a counterstain, as its ultraviolet (max 358 nm) excitation and blue (max 461 nm) emission wavelengths separate it nicely from many popular primary fluorophores. It can be used on either fixed or live cells, although its low permeability in live cells demands that higher concentrations be used.

Chemical Properties

Yellow Solid

Uses

DAPI is used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex and as a cell permeable fluorescent minor groove-binding probe for DNA. For staining DNA in agarose gels

Uses

Suitable for

  • DNA staining in agarose gels
  • analysis of changes in DNA during apoptosis
  • detection of mycoplasma
  • photofootprinting of DNA
  • immunofluorescent staining of cells

DAPI has been used:-
  • in rapid monitoring of microbial contamination
  • in chromosomal banding technique
  • in detection of apoptotic cells
  • in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA
  • to stain mature pollen grains(0.5 mg/ml)

Uses

4',6-Diamidino-2-phenylindole dihydrochloride is used as a cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine.It is used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes.

General Description

DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

storage

-20°C

References

[1] W. C. RUSSELL D. H W CAROL NEWMAN. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses[J]. Nature, 1975, 253 5491: 461-462. DOI:10.1038/253461a0.
[2] BETTY I. TARNOWSKI James H N F Spinale. DAPI as a useful stain for nuclear quantitation.[J]. Biotechnic & Histochemistry, 1991, 22 1: 297-302. DOI:10.3109/10520299109109990.
[3] ANA KATRINA ESTANDARTE. The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes.[J]. Scientific Reports, 2016: 31417. DOI:10.1038/srep31417.

4',6-Diamidino-2-phenylindole dihydrochloride Preparation Products And Raw materials

Raw materials

Preparation Products

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4',6-Diamidino-2-phenylindole dihydrochloride Suppliers

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View Lastest Price from 4',6-Diamidino-2-phenylindole dihydrochloride manufacturers

Hebei Mojin Biotechnology Co., Ltd
Product
4',6-Diamidino-2-phenylindole dihydrochloride 28718-90-3
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
50000KG/month
Release date
2023-09-28
Career Henan Chemical Co
Product
4',6-Diamidino-2-phenylindole dihydrochloride 28718-90-3
Price
US $1.00/KG
Min. Order
1KG
Purity
98%
Supply Ability
1
Release date
2018-08-06

28718-90-3, 4',6-Diamidino-2-phenylindole dihydrochlorideRelated Search:

Indole-3-butyric acid FORMAMIDINE HYDROCHLORIDE Indometacin Indole-3-acetic acid Dihydrochloride 1H-INDOLE-6-METHANAMINE Benzamidine hydrochloride 6-Methylindole 4-Methylbenzene-1-carboximidamide hydrochloride 4-Methylbenzylamine BENZAMIDINE 2-Phenylindole Acetamidine hydrochloride 4',6-DIAMIDINO-2-PHENYLINDOLE DIHYDROCHLORIDE N-HYDRATE,4',6-DIAMIDINO-2-PHENYLINDOLE DIHYDROCHLORIDE: HYDRATE,DAPI DIHYDROCHLORIDE HYDRATE,DAPI 1H-Indole, 2,6-dimethyl- 4-METHYL-BENZAMIDINE 2-PHENYLPYRROLE DAPI

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