Discovery Properties Clinical implications Regulation of synthesis and release Uses

OPN

Discovery Properties Clinical implications Regulation of synthesis and release Uses
Product Name
OPN
Chemical Name
OPN
Synonyms
OPN;OSTP;SPP1;BOVINE OPN;OSTEOPONTIN;Nephropontin;BOVINE OSTEOPONTIN;BONE SIALOPROTEIN-1;OSTEOPONTIN, BOVINE;OSTEOPONTIN (HUMAN)
CBNumber
CB0332095
Formula Weight
0
MOL File
Mol file
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OPN Property

storage temp. 
-20°C
form 
lyophilized powder
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Safety

RIDADR 
UN 3264 8 / PGIII
WGK Germany 
3
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H290May be corrosive to metals

Precautionary statements

P234Keep only in original container.

P390Absorb spillage to prevent material damage.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
SRP3131
Product name
Osteopontin human
Purity
recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture
Packaging
50μg
Price
$358
Updated
2024/03/01
Sigma-Aldrich
Product number
SAB4502841
Product name
Anti-Osteopontin, C-Terminal antibody produced in rabbit
Purity
affinity isolated antibody
Packaging
100μg
Price
$506
Updated
2024/03/01
Sigma-Aldrich
Product number
SAB1306579
Product name
ANTI-OPN-A/B(N-TERMINAL) antibody produced in rabbit
Purity
affinity isolated antibody, buffered aqueous solution
Packaging
400μl
Price
$481
Updated
2024/03/01
Sigma-Aldrich
Product number
RAB0437
Product name
Mouse Osteopontin ELISA Kit
Purity
for serum, plasma and cell culture supernatant
Packaging
1KIT
Price
$552
Updated
2024/03/01
Sigma-Aldrich
Product number
RAB0436
Product name
Human Osteopontin ELISA Kit
Purity
for serum, plasma, cell culture supernatant and urine
Packaging
1kit
Price
$552
Updated
2024/03/01
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OPN Chemical Properties,Usage,Production

Discovery

Osteopontin (OPN) is a highly phosphorylated glycoprotein consisting of approximately 314 aa. It was first identified in 1985 by Franzen and Heinegård as sialoprotein isolated from bovine bone matrix.

Properties

A unit of mass of nascent OPN is 33kDa in humans and 32kDa in mice.It is expressed as a sequence of about 300 aa (305 in zebrafish, 264 in chickens, 294 in mice, 314 in humans). However, the apparent molecular weight of OPN according to SDS-PAGE can range from 45 to 75kDa because of posttranslational modifications such as O-linked glycosylation, tyrosine sulfation and sialylation, and multiple Ser/Thr phosphorylations. Those modifications vary according to the cellular origin of OPN and greatly affect the function of the protein.4 Basal pI 4.37. Soluble in water. Stable in 1xPBS at 4°C within 2–4weeks.

Clinical implications

Increased OPN secretion has been correlated with cell proliferation in various malignancies, including breast and prostate cancer, squamous cell carcinoma, melanoma, osteosarcoma, and glioblastoma.

Regulation of synthesis and release

OPN is constitutively expressed in many cells but is upregulated under various conditions, particularly in inflammation and cancer progression. The following variants of growth factors and cytokines (including LPS, NO, Ang II, IL-1b, IL-2, IL-3, IFN-γ, TNF-α, TGF-β) have been reported to upregulate OPN expression.

Uses

OPN produced by tumor cells is structurally different compared to that produced by normal cells. Therefore, it has been reported that blocking the production of OPN by using antibodies or synthetic peptides that recognize specific epitopes of tumor-derived OPN may block cell proliferation.

Uses

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

General Description

The Human OPN ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human OPN in serum, plasma, cell culture supernatants and urine.

Biochem/physiol Actions

Osteopontin (OPN) is a secreted glycoprotein and is assumed to be associated with various cellular processes such as adhesion, migration, and biomineralization in diverse tissues. Osteopontin acts as a ligand to αvβ3 integrin and possibly other receptors. In addition to its involvement in mineralized matrix formation, osteopontin can also function as a cytokine that stimulates the release of IFNγ (interferon γ) and IL-12 (interleukin 12), while inhibiting the production of IL-10. Osteopontin is involved in inflammation and also regulates immunity against infectious diseases. It also co-stimulates T cell proliferation. Osteopontin has proinflammatory functions and is associated with multiple sclerosis. It participates in inflammation in Crohn′s Disease. Osteopontin plays a crucial role in bone remodelling and retards ectopic calcification by inhibiting the formation of hydroxyapatite. OPN is thought to prevent renal stone formation by suppressing nucleation, growth and aggregation of calcium oxalate crystals.

OPN Preparation Products And Raw materials

Raw materials

Preparation Products

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OPN Suppliers

Sigma-Aldrich
Tel
021-61415566 800-8193336
Email
orderCN@merckgroup.com
Country
China
ProdList
51456
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Beijing Solarbio Science & Tecnology Co., Ltd.
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010-50973130 4009686088
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68
Wuhan Chemstan Biotechnology Co., Ltd.
Tel
027-65317797 15926423062
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QQ:422450190
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422450190@qq.com
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China
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Shanghai Universal Biotech Co.,Ltd
Tel
13774214275
Email
zhouzz@univ-bio.com
Country
China
ProdList
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Advantage
58
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Tel
021-34661275 15301693058
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yajikit@163.com
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China
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58
Shanghai Huzhen Industrial Co., LTD
Tel
021-60345367 13916550749
Email
sales@shzbio.com
Country
China
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Beijing Boorsen Biotechnology Co., Ltd
Tel
400-901-9800 18611424007
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cis@bioss.com.cn
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OPNRelated Search:


  • Anti-Osteopontin, C-Terminal antibody produced in rabbit
  • ANTI-OPN-A/B(N-TERMINAL) antibody produced in rabbit
  • OPN
  • BOVINE OSTEOPONTIN
  • BONE SIALOPROTEIN-1
  • EARLY T LYMPHOCYTE ACTIVATION PROTEIN-1 (ETA-1)
  • BOVINE OPN
  • Osteopontin from bovine milk
  • SECRETED PHOSPHOPROTEIN 1 (SPP1)
  • OSTEOPONTIN, BOVINE
  • OSTEOPONTIN
  • OSTEOPONTIN (HUMAN)
  • Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
  • Osteopontin from mouse
  • OSTEOPONTIN, HUMAN MILK
  • Nephropontin
  • OSTP
  • Mouse Osteopontin ELISA Kit
  • SPP1
  • Human Osteopontin ELISA Kit
  • Recombinant Human SPP1 Protein, His Tag
  • Rabbit Anti-Osteopontin antibody
  • 多肽抗原