Discovery Properties Clinical implications Regulation of synthesis and release Uses

OPN

Discovery Properties Clinical implications Regulation of synthesis and release Uses
Product Name
OPN
Chemical Name
OPN
Synonyms
OPN;OSTP;SPP1;BOVINE OPN;OSTEOPONTIN;Nephropontin;BOVINE OSTEOPONTIN;BONE SIALOPROTEIN-1;OSTEOPONTIN, BOVINE;OSTEOPONTIN (HUMAN)
CBNumber
CB0332095
Formula Weight
0
MOL File
Mol file
More
Less

OPN Property

storage temp. 
-20°C
form 
lyophilized powder
More
Less

Safety

RIDADR 
UN 3264 8 / PGIII
WGK Germany 
3
More
Less

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H290May be corrosive to metals

Precautionary statements

P234Keep only in original container.

P390Absorb spillage to prevent material damage.

More
Less

N-Bromosuccinimide Price

Sigma-Aldrich
Product number
SRP3131
Product name
Osteopontin human
Purity
recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture
Packaging
50μg
Price
$358
Updated
2024/03/01
Sigma-Aldrich
Product number
SAB4502841
Product name
Anti-Osteopontin, C-Terminal antibody produced in rabbit
Purity
affinity isolated antibody
Packaging
100μg
Price
$506
Updated
2024/03/01
Sigma-Aldrich
Product number
SAB1306579
Product name
ANTI-OPN-A/B(N-TERMINAL) antibody produced in rabbit
Purity
affinity isolated antibody, buffered aqueous solution
Packaging
400μl
Price
$481
Updated
2024/03/01
Sigma-Aldrich
Product number
RAB0437
Product name
Mouse Osteopontin ELISA Kit
Purity
for serum, plasma and cell culture supernatant
Packaging
1KIT
Price
$552
Updated
2024/03/01
Sigma-Aldrich
Product number
RAB0436
Product name
Human Osteopontin ELISA Kit
Purity
for serum, plasma, cell culture supernatant and urine
Packaging
1kit
Price
$552
Updated
2024/03/01
More
Less

OPN Chemical Properties,Usage,Production

Discovery

Osteopontin (OPN) is a highly phosphorylated glycoprotein consisting of approximately 314 aa. It was first identified in 1985 by Franzen and Heinegård as sialoprotein isolated from bovine bone matrix.

Properties

A unit of mass of nascent OPN is 33kDa in humans and 32kDa in mice.It is expressed as a sequence of about 300 aa (305 in zebrafish, 264 in chickens, 294 in mice, 314 in humans). However, the apparent molecular weight of OPN according to SDS-PAGE can range from 45 to 75kDa because of posttranslational modifications such as O-linked glycosylation, tyrosine sulfation and sialylation, and multiple Ser/Thr phosphorylations. Those modifications vary according to the cellular origin of OPN and greatly affect the function of the protein.4 Basal pI 4.37. Soluble in water. Stable in 1xPBS at 4°C within 2–4weeks.

Clinical implications

Increased OPN secretion has been correlated with cell proliferation in various malignancies, including breast and prostate cancer, squamous cell carcinoma, melanoma, osteosarcoma, and glioblastoma.

Regulation of synthesis and release

OPN is constitutively expressed in many cells but is upregulated under various conditions, particularly in inflammation and cancer progression. The following variants of growth factors and cytokines (including LPS, NO, Ang II, IL-1b, IL-2, IL-3, IFN-γ, TNF-α, TGF-β) have been reported to upregulate OPN expression.

Uses

OPN produced by tumor cells is structurally different compared to that produced by normal cells. Therefore, it has been reported that blocking the production of OPN by using antibodies or synthetic peptides that recognize specific epitopes of tumor-derived OPN may block cell proliferation.

Uses

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

General Description

The Human OPN ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human OPN in serum, plasma, cell culture supernatants and urine.

Biochem/physiol Actions

Osteopontin (OPN) is a secreted glycoprotein and is assumed to be associated with various cellular processes such as adhesion, migration, and biomineralization in diverse tissues. Osteopontin acts as a ligand to αvβ3 integrin and possibly other receptors. In addition to its involvement in mineralized matrix formation, osteopontin can also function as a cytokine that stimulates the release of IFNγ (interferon γ) and IL-12 (interleukin 12), while inhibiting the production of IL-10. Osteopontin is involved in inflammation and also regulates immunity against infectious diseases. It also co-stimulates T cell proliferation. Osteopontin has proinflammatory functions and is associated with multiple sclerosis. It participates in inflammation in Crohn′s Disease. Osteopontin plays a crucial role in bone remodelling and retards ectopic calcification by inhibiting the formation of hydroxyapatite. OPN is thought to prevent renal stone formation by suppressing nucleation, growth and aggregation of calcium oxalate crystals.

OPN Preparation Products And Raw materials

Raw materials

Preparation Products

More
Less

OPN Suppliers

Sigma-Aldrich
Tel
021-61415566 800-8193336
Email
orderCN@merckgroup.com
Country
China
ProdList
51456
Advantage
80
Beijing Solarbio Science & Tecnology Co., Ltd.
Tel
010-50973130 4009686088
Email
3193328036@qq.com
Country
China
ProdList
29785
Advantage
68
Wuhan Chemstan Biotechnology Co., Ltd.
Tel
027-65317797 15926423062
Fax
QQ:422450190
Email
422450190@qq.com
Country
China
ProdList
10351
Advantage
58
Shanghai Universal Biotech Co.,Ltd
Tel
13774214275
Email
gaojun@univ-bio.com
Country
China
ProdList
24990
Advantage
58
Shanghai Yaji Biological Technology Co., Ltd.
Tel
021-34661275 15301693058
Email
yajikit@163.com
Country
China
ProdList
9632
Advantage
58
Shanghai Huzhen Industrial Co., LTD
Tel
021-60345367 13916550749
Email
sales@shzbio.com
Country
China
ProdList
9966
Advantage
58
Beijing Boorsen Biotechnology Co., Ltd
Tel
400-901-9800 18611424007
Email
cis@bioss.com.cn
Country
China
ProdList
9518
Advantage
58
Ningbo Mingzhou Biotechnology Co., Ltd
Tel
--
Fax
--
Email
mingzhoubio@163.com
Country
CHINA
ProdList
6974
Advantage
58
Shanghai Tongwei Biotechnology Co., Ltd
Tel
--
Fax
--
Email
253437001@qq.com
Country
CHINA
ProdList
6377
Advantage
58
Shanghai Xuanya Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
1517347860@qq.com
Country
CHINA
ProdList
6673
Advantage
58
Shanghai Yanmu Industrial Co., Ltd.
Tel
--
Fax
--
Email
2095001174@qq.com
Country
CHINA
ProdList
6722
Advantage
58
Shanghai Yansheng Industrial Co., Ltd.
Tel
--
Fax
--
Email
shyssw@126.com
Country
CHINA
ProdList
6729
Advantage
58
Shanghai Guyan Industrial Co., Ltd.
Tel
--
Fax
--
Email
3004901493@qq.com
Country
CHINA
ProdList
6817
Advantage
58
Shanghai Yuanmu Biological Technology Co., Ltd.
Tel
--
Fax
--
Country
CHINA
ProdList
6179
Advantage
58
Shenyang Wanji Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
sales@wanleibio.com
Country
CHINA
ProdList
389
Advantage
58
Shanghai Bangjing Industrial Co., Ltd.
Tel
--
Fax
--
Email
3004972055@qq.com
Country
CHINA
ProdList
6538
Advantage
58
Shanghai Guduo Biological Technology Co., Ltd.
Tel
--
Fax
--
Country
CHINA
ProdList
6266
Advantage
58
Wuhan Aibotek Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
tech@abclonal.com
Country
CHINA
ProdList
6796
Advantage
58
Beijing Boaolong Immunology Technology Co., Ltd.
Tel
--
Fax
--
Country
CHINA
ProdList
5676
Advantage
58
Shanghai Qunji Biological Technology Co., Ltd.
Tel
--
Fax
--
Country
CHINA
ProdList
6971
Advantage
58
Beijing Huaxia Ocean Technology Co., Ltd.
Tel
--
Fax
--
Email
huaxiabio@163.com
Country
CHINA
ProdList
6122
Advantage
58
Shanghai Qumai Biological Technology Co., Ltd.
Tel
--
Fax
--
Email
13770640776@189.cn
Country
CHINA
ProdList
6227
Advantage
58
Shanghai Boyi Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
15800446246@163.com
Country
CHINA
ProdList
5416
Advantage
58
Xi Shi Biotechnology (Shanghai) Co., Ltd.
Tel
--
Fax
--
Email
joanna.lu@csbiochina.com
Country
CHINA
ProdList
2590
Advantage
58
Nanjing Shanben Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
sales@sciben.com
Country
CHINA
ProdList
6053
Advantage
58
Beijing Taize Ruida Technology Co., Ltd.
Tel
--
Fax
--
Email
taizeorder@163.com
Country
CHINA
ProdList
6536
Advantage
58
Shanghai Jingke Chemical Technology Co., Ltd.
Tel
--
Fax
--
Email
jingkehuaxue@163.com
Country
CHINA
ProdList
6972
Advantage
58
Nanjing Sai Ruirui Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
sales@shrbio.com
Country
CHINA
ProdList
6871
Advantage
58

OPNRelated Search:


  • Anti-Osteopontin, C-Terminal antibody produced in rabbit
  • ANTI-OPN-A/B(N-TERMINAL) antibody produced in rabbit
  • OPN
  • BOVINE OSTEOPONTIN
  • BONE SIALOPROTEIN-1
  • EARLY T LYMPHOCYTE ACTIVATION PROTEIN-1 (ETA-1)
  • BOVINE OPN
  • Osteopontin from bovine milk
  • SECRETED PHOSPHOPROTEIN 1 (SPP1)
  • OSTEOPONTIN, BOVINE
  • OSTEOPONTIN
  • OSTEOPONTIN (HUMAN)
  • Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
  • Osteopontin from mouse
  • OSTEOPONTIN, HUMAN MILK
  • Nephropontin
  • OSTP
  • Mouse Osteopontin ELISA Kit
  • SPP1
  • Human Osteopontin ELISA Kit
  • Recombinant Human SPP1 Protein, His Tag
  • Rabbit Anti-Osteopontin antibody
  • 多肽抗原