THROMBIN Property

storage temp. 

−20°C

solubility 

H₂O: 1 mL/vial clear to slightly hazy, colorless solution

Colour Index 

52000

form 

powder

color 

white

Merck 

9346

CAS DataBase Reference

581-64-6(CAS DataBase Reference)

EPA Substance Registry System

Thionin (581-64-6)

Safety

Hazard Codes 

Xn,B

Risk Statements 

36/37/38-42

Safety Statements 

22-24-26-36/37

WGK Germany 

2

RTECS 

XO8950000

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H312Harmful in contact with skin

H332Harmful if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P312Call a POISON CENTER or doctor/physician if you feel unwell.

P322Specific measures (see …on this label).

P330Rinse mouth.

P363Wash contaminated clothing before reuse.

P501Dispose of contents/container to..…

THROMBIN Chemical Properties,Usage,Production

Description

Thrombin is the last enzyme in the clotting cascade functioning to cleave fibrinogen to fibrin and potentiates the procoagulation process by activating factors V, VIII, XI, and XIII. Conversely, thrombin aids the anticoagulant process by activating protein C. Activated protein C inhibits activated factors V and VIII ultimately down-regulating further thrombin production. Thrombin is often used as a potent platelet activator in vitro. Platelet responses to thrombin are mediated through protease-activated receptors (PARs). Thrombin-mediated signaling regulates a diverse range of metabolic processes including activation of phospholipases in platelets, endothelial cells, and cardiac myocytes.

Chemical Properties

dark green to black powder or crystals

Uses

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess in vitro hemostatic properties of French lyophilized plasma.

Uses

Thionine Chloride is used in the preparation of carbon paste electrode with covalently immobilized thionine for electrochemical sensing of hydrogen peroxide

Preparation

The first thiazine dye, named after its dis coverer, was Lauth's Violet, which was obtained in 1876 by joint oxidation of 1,4-phenylenediamine and hydrogen sulfide with iron(III) chloride.

Definition

ChEBI: An organic chloride salt composed of 3,7-diaminophenothiazin-5-ium and chloride ions in a 1:1 ratio. A strongly metachromatic dye, useful for the staining of acid mucopolysaccharides. It is also a common nuclear stain and can be used for the demonstration f Nissl substance in nerve cells of the CNS.

General Description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. Thrombin is also known as coagulation factor IIa. It is a serine endopeptidase that hydrolyzes peptide and ester bonds specifically at the carboxylic side of arginine. This enzyme converts fibrinogen to fibrin.