Tobramycin sulfate
- Product Name
- Tobramycin sulfate
- CAS No.
- 49842-07-1
- Chemical Name
- Tobramycin sulfate
- Synonyms
- obracine;tenemicin;Tobramycin sulfate;TOBRAMYCIN SULPHATE;TobraMycin Sulfate API;1-epitobramycinsulfate;TOBRAMYCIN SULFATE SALT;Tobramycin Monosulphate Salt;TobraMycin sulfate research grade;d-6-trideoxy-alpha-d-ribohexopyranosyl-(1-4))-2-deoxysulfate(salt)
- CBNumber
- CB0475411
- Molecular Formula
- C18H39N5O13S
- Formula Weight
- 565.59
- MOL File
- 49842-07-1.mol
Tobramycin sulfate Property
- storage temp.
- 2-8°C
- solubility
- Methanol (Slightly), Water (Sparingly)
- form
- Powder
- color
- White to off-white
- Water Solubility
- Soluble in water (50 mg/ml).
- Stability:
- Hygroscopic
- CAS DataBase Reference
- 49842-07-1
- EPA Substance Registry System
- Tobramycin sulfate (49842-07-1)
Safety
- Hazard Codes
- T
- Risk Statements
- 61-20/21/22
- Safety Statements
- 53-22-36/37/39-45
- WGK Germany
- 3
- HS Code
- 2941900000
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H302Harmful if swallowed
H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P270Do not eat, drink or smoke when using this product.
P271Use only outdoors or in a well-ventilated area.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352IF ON SKIN: wash with plenty of soap and water.
P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P312Call a POISON CENTER or doctor/physician if you feel unwell.
P330Rinse mouth.
P403+P233Store in a well-ventilated place. Keep container tightly closed.
N-Bromosuccinimide Price
- Product number
- J62995
- Product name
- Tobramycin sulfate
- Packaging
- 100mg
- Price
- $56
- Updated
- 2024/03/01
- Product number
- J62995
- Product name
- Tobramycin sulfate
- Packaging
- 1g
- Price
- $350
- Updated
- 2024/03/01
- Product number
- T524028
- Product name
- TobramycinMonosulphateSalt
- Packaging
- 100mg
- Price
- $45
- Updated
- 2021/12/16
- Product number
- 290614
- Product name
- Tobramycin sulfate
- Packaging
- 50g
- Price
- $672
- Updated
- 2021/12/16
- Product number
- BIT0153
- Product name
- Tobramycinsulphate
- Packaging
- 500mg
- Price
- $44
- Updated
- 2021/12/16
Tobramycin sulfate Chemical Properties,Usage,Production
Originator
Distobram,Lilly,Portugal
Uses
Nebcin (Lilly).
Uses
Tobramycin sulfate is used as an antibiotic which is used to treat various types of bacterial infections, particularly Gram-negative infections. It is especially effective against species of Pseudomonas. It is used to inhibit bacterial protein synthesis at the level of 30S (16S rRNA) and 70S ribosomal complex assembly. It is used to treat pseudomonas aeruginosa lung infections and is used in combination with other antibiotics to treat urinary tract infections, gynecologic infections, peritonitis, endocarditis, pneumonia, sepsis, respiratory infections, osteomyelitis and other soft-tissue infections. It is a potential therapy for sinus infections1. Product T1783 has been used to study antibiotic resistance2.
Manufacturing Process
Two thousand parts by volume of an aqueous culture medium (pH 7.2) comprising 0.5% of glycerol, 0.5% of polypeptone, 0.5% of yeast extract and 0.3% of meat extract is inoculated with Escherichia coli R11 (IFO-13560). The medium is incubated at 37°C under aeration for 18 h. The culture broth is subjected to centrifuge to recover 4.4 parts of wet cells. The cells are suspended into 17.6 parts by volume of 0.05 M phosphate buffer (pH 7.0). The suspension is subjected to ultrasonic oscillation (Kaijo Denki Co., Ltd.; TA-4201, 4280-type, 2A) to disintegrate the cells, followed by removing the debris (insoluble materials) by centrifugation, whereby 17 parts by volume of crude enzyme solution is obtained. To 17 parts by volume of the crude enzyme solution are added 5 parts of kanamycin B, 50 parts by volume of 0.5 M phosphate buffer (pH 7.0), 100 parts by volume of 1 M adenosine triphosphate solution, 50 parts by volume of 0.1 M magnesium acetate solution and 50 parts by volume of 0.1 M 2- mercaptoethanol, which is filled up to 500 parts by volume with distilled water. The mixture is subjected to enzymic reaction at 37°C for 20 h. The reaction mixture is heated at 80°C for 5 min to cease the reaction, followed by centrifugation. The supernatant is run onto a column of 100 parts by volume of cation-exchange resin [Amberlite IRC-50, NH4 +-form]. The column is washed with water, and then eluted with 1 N-aqueous ammonia to give fractions which contain kanamycin B-3'-phosphate. The fractions are collected and concentrated under reduced pressure, and then the concentrate is run onto a column of 100 parts by volume of cation-exchange resin [carboxy-methyl Sephadex C-25, NH4 +-form]. The column is washed with water, and eluted with 0.2 N-aqueous ammonia to give fractions which contain kanamycin B-3'-phosphate. The fractions are collected, concentrated and lyophilized, whereby 4.5 parts of kanamycin B-3'-phosphate. A solution of one part of kanamycin B-3'-phosphate, 10 parts by volume of bis(trimethylsilyl)acetamide, 2 parts by volume of trimethylchlorosilane and 0.4 part of triphenylphosphine is heated at 115°C for 30 h. After cooling, the reaction mixture is concentrated under reduced pressure, and to the concentrate is added 100 parts by volume of methanol and 50 parts by volume of water, and then the mixture is stirred for 1 h. Methanol is removed by distillation, and ethyl acetate-soluble portion is removed. The water layer is run onto a column of 60 parts by volume of cation-exchange resin [Amberlite CG-50, NH4 +-form]. The column is washed with 200 parts by volume of water, and fractionated by linear gradient method with 600 parts by volume of water and 600 parts by volume of 0.5 N-aqueous ammonia, each fraction being 10 parts by weight. Upon concentration of some fractions 0.61 part of 2',3'- epimino-2'-deamino-3'-deoxykanamycin B is obtained. In 40 parts by volume of water is dissolved 0.6 part of 2',3'-epimino-2'- deamino-3'-deoxykanamycin B, and in the presence of 9 parts by volume of Raney nickel the mixture is stirred while introducing hydrogen gas at a pressure of 100 kg/cm2 at 60°C for 6 h. After the reaction Raney nickel is separated by filtration. The Raney nickel is washed well with 300 parts by volume of 1 N-aqueous ammonia and the washing is added to the filtrate. The whole is concentrated to about 100 parts by volume. The precipitated insolubles are removed by filtration, and the pH of the supernatant is adjusted to about 5.0 with hydrochloric acid. The mixture is run onto a column of 50 ml of cation-exchange resin [Amberlite CG-50, NH4 +-form]. The column is washed with 150 parts by volume of water, and fractionated by linear gradient method with 1400 parts by volume of water and 1400 parts by volume of 0.3 N-aqueous ammonia, each fraction being 14 parts by weight. From No. 146 to 162 fractions 0.30 part of 3'-deoxykanamycin B (Tobramycin) is obtained. water. The mixture is subjected to enzymic reaction at 37°C for 20 h. The reaction mixture is heated at 80C for 5 min to cease the reaction, followed by centrifugation. The supernatant is run onto a column of 100 parts by volume of cation-exchange resin [Amberlite IRC-50, NH4 +-form]. The column is washed with water, and then eluted with 1 N-aqueous ammonia to give fractions which contain kanamycin B-3'-phosphate. The fractions are collected and concentrated under reduced pressure, and then the concentrate is run onto a column of 100 parts by volume of cation-exchange resin [carboxy-methyl Sephadex C-25, NH4 +-form]. The column is washed with water, and eluted with 0.2 N-aqueous ammonia to give fractions which contain kanamycin B-3'-phosphate. The fractions are collected, concentrated and lyophilized, whereby 4.5 parts of kanamycin B-3'-phosphate. A solution of one part of kanamycin B-3'-phosphate, 10 parts by volume of bis(trimethylsilyl)acetamide, 2 parts by volume of trimethylchlorosilane and 0.4 part of triphenylphosphine is heated at 115°C for 30 h. After cooling, the reaction mixture is concentrated under reduced pressure, and to the concentrate is added 100 parts by volume of methanol and 50 parts by volume of water, and then the mixture is stirred for 1 h. Methanol is removed by distillation, and ethyl acetate-soluble portion is removed. The water layer is run onto a column of 60 parts by volume of cation-exchange resin [Amberlite CG-50, NH4 +-form]. The column is washed with 200 parts by volume of water, and fractionated by linear gradient method with 600 parts by volume of water and 600 parts by volume of 0.5 N-aqueous ammonia, each fraction being 10 parts by weight. Upon concentration of some fractions 0.61 part of 2',3'- epimino-2'-deamino-3'-deoxykanamycin B is obtained. In 40 parts by volume of water is dissolved 0.6 part of 2',3'-epimino-2'- deamino-3'-deoxykanamycin B, and in the presence of 9 parts by volume of Raney nickel the mixture is stirred while introducing hydrogen gas at a pressure of 100 kg/cm2 at 60°C for 6 h. After the reaction Raney nickel is separated by filtration. The Raney nickel is washed well with 300 parts by volume of 1 N-aqueous ammonia and the washing is added to the filtrate. The whole is concentrated to about 100 parts by volume. The precipitated insolubles are removed by filtration, and the pH of the supernatant is adjusted to about 5.0 with hydrochloric acid. The mixture is run onto a column of 50 ml of cation-exchange resin [Amberlite CG-50, NH4 +-form]. The column is washed with 150 parts by volume of water, and fractionated by linear gradient method with 1400 parts by volume of water and 1400 parts by volume of 0.3 N-aqueous ammonia, each fraction being 14 parts by weight. From No. 146 to 162 fractions 0.30 part of 3'-deoxykanamycin B is obtained. The aqueous solution containing 3'-deoxykanamycin B in free base form by addition of concentrated sulfuric acid give the 3'-deoxykanamycin B sulfate (tobramycin sulfate). The solution of this compound is decolorized by stirring of Darco G-60, filtered and purified by column chromatography.
Therapeutic Function
Antibiotic
Clinical Use
Introduced in 1976, tobramycin sulfate (Nebcin) is the mostactive of the chemically related aminoglycosides called nebramycinsobtained from a strain of Streptomyces tenebrarius Five members of the nebramycin complex have beenidentified chemically.
Factors 4 and 4＇ are 6＂-O-carbamoylkanamycin B andkanamycin B, respectively; factors 5＇ and 6 are 6＂-O-carbamoyltobramycinand tobramycin; and factor 2 isapramycin, a tetracyclic aminoglycoside with an unusual bicycliccentral ring structure. Kanamycin B and tobramycinprobably do not occur in fermentation broths per se but areformed by hydrolysis of the 6-O＂-carbamoyl derivatives inthe isolation procedure.
The most important property of tobramycin is its activityagainst most strains of P. aeruginosa, exceeding that of gentamicinby twofold to fourfold. Some gentamicin-resistantstrains of this troublesome organism are sensitive to tobramycin,but others are resistant to both antibiotics. OtherGram-negative bacilli and staphylococci are generally moresensitive to gentamicin. Tobramycin more closely resembleskanamycin B in structure (it is 3＇-deoxykanamycin B).
Tobramycin sulfate Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from Tobramycin sulfate manufacturers
- Product
- Tobramycin sulfate 49842-07-1
- Price
- US $1.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 10000KGS
- Release date
- 2020-01-02