ChemicalBook > CAS DataBase List > RED BLOOD CELL LYSING BUFFER HYBRI-MAX

RED BLOOD CELL LYSING BUFFER HYBRI-MAX

Product Name: RED BLOOD CELL LYSING BUFFER HYBRI-MAX
Chemical Name: RED BLOOD CELL LYSING BUFFER HYBRI-MAX
Synonyms: RBC Lysing Buffer;Red Cell Lysis Buffer;RED BLOOD CELL LYSING BUFFER HYBRI-MAX
CBNumber: CB0566979
Formula Weight: 0
MOL File: Mol file

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RED BLOOD CELL LYSING BUFFER HYBRI-MAX Property

form: liquid

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Safety

WGK Germany: 3

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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: R7757
Product name: Red Blood Cell Lysing Buffer Hybri-Max<SUP>™</SUP>
Purity: liquid, sterile-filtered, suitable for hybridoma
Packaging: 100 mL
Price: $28.5
Updated: 2025/07/31

Sigma-Aldrich

Product number: R7757
Product name: Red Blood Cell Lysing Buffer Hybri-Max™
Purity: liquid, sterile-filtered, suitable for hybridoma
Packaging: 100ML
Price: $28.1
Updated: 2023/06/20

American Custom Chemicals Corporation

Product number: BBF0000082
Product name: RBC LYSING BUFFER
Purity: 95.00%
Packaging: 5MG
Price: $497.08
Updated: 2021/12/16

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RED BLOOD CELL LYSING BUFFER HYBRI-MAX Chemical Properties,Usage,Production

Uses

Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 × g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated.

Biochem/physiol Actions

Red Blood Cell Lysing Buffer has been developed for use in hybridoma protocols to remove red blood cells from mouse splenocyte suspensions before fusion. It is also useful in systems where it may be desirable to remove red blood cells from cell suspensions, such as whole blood.

RED BLOOD CELL LYSING BUFFER HYBRI-MAX Preparation Products And Raw materials

Raw materials

Preparation Products

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RED BLOOD CELL LYSING BUFFER HYBRI-MAXRelated Search:

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RED BLOOD CELL LYSING BUFFER HYBRI-MAX

Red Cell Lysis Buffer

RBC Lysing Buffer