RED BLOOD CELL LYSING BUFFER HYBRI-MAX

Product Name
RED BLOOD CELL LYSING BUFFER HYBRI-MAX
Chemical Name
RED BLOOD CELL LYSING BUFFER HYBRI-MAX
Synonyms
RBC Lysing Buffer;Red Cell Lysis Buffer;RED BLOOD CELL LYSING BUFFER HYBRI-MAX
CBNumber
CB0566979
Formula Weight
0
MOL File
Mol file
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RED BLOOD CELL LYSING BUFFER HYBRI-MAX Property

form 
liquid
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Safety

WGK Germany 
3
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
R7757
Product name
Red Blood Cell Lysing Buffer Hybri-Max™
Purity
liquid, sterile-filtered, suitable for hybridoma
Packaging
100ML
Price
$28.1
Updated
2023/06/20
American Custom Chemicals Corporation
Product number
BBF0000082
Product name
RBC LYSING BUFFER
Purity
95.00%
Packaging
5MG
Price
$497.08
Updated
2021/12/16
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RED BLOOD CELL LYSING BUFFER HYBRI-MAX Chemical Properties,Usage,Production

Uses

Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 × g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated.

Biochem/physiol Actions

Red Blood Cell Lysing Buffer has been developed for use in hybridoma protocols to remove red blood cells from mouse splenocyte suspensions before fusion. It is also useful in systems where it may be desirable to remove red blood cells from cell suspensions, such as whole blood.

RED BLOOD CELL LYSING BUFFER HYBRI-MAX Preparation Products And Raw materials

Raw materials

Preparation Products

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  • RED BLOOD CELL LYSING BUFFER HYBRI-MAX
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