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ABTS

Product Name
ABTS
CAS No.
30931-67-0
Chemical Name
ABTS
Synonyms
ABTS;diammonium salt;ABTS DIAMMONIUM SALT;ABTS;ABTS(TM);ABTS ine-6-suL;ABTS-(NH4)2;AzBTS-(NH4)2;ABTS SUBSTRATE
CBNumber
CB0682264
Molecular Formula
C18H24N6O6S4
Formula Weight
548.68
MOL File
30931-67-0.mol
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ABTS Property

Melting point:
>181oC (dec.)
storage temp. 
2-8°C
solubility 
H2O: 50 mg/mL, very slightly hazy, green
form 
tablet
color 
Pale Green to Light Green
PH
pH(50g/l, 25℃) : 5.0~6.0
Water Solubility 
Dissolve in water at 50mg/ml. Solution may be slightly hazy.
Sensitive 
Light Sensitive
BRN 
7329461
Stability:
Hygroscopic, Light Sensitive
InChIKey
OHDRQQURAXLVGJ-AXMZSLBLSA-N
SMILES
C12C=CC(S(=O)(=O)O)=CC=1S/C(=N\N=C1/SC3C=C(S(=O)(=O)O)C=CC=3N/1CC)/N2CC.N.N
CAS DataBase Reference
30931-67-0(CAS DataBase Reference)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36
WGK Germany 
3
RTECS 
DL7002000
8-10-23
HS Code 
29342080
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P405Store locked up.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
A9941
Product name
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt
Purity
tablet, 10 mg substrate per tablet
Packaging
5 tabs
Price
$59.8
Updated
2025/07/31
Sigma-Aldrich
Product number
A9941
Product name
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt
Purity
tablet, 10 mg substrate per tablet
Packaging
50 tabs
Price
$230
Updated
2025/07/31
Sigma-Aldrich
Product number
A9941
Product name
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt
Purity
tablet, 10 mg substrate per tablet
Packaging
100 tabs
Price
$400
Updated
2025/07/31
Sigma-Aldrich
Product number
194430
Product name
ABTS™ Chromophore, Diammonium Salt
Packaging
1g
Price
$166
Updated
2025/07/31
Sigma-Aldrich
Product number
11557
Product name
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt
Purity
≥98.0% (HPLC)
Packaging
1g
Price
$87.8
Updated
2025/07/31
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ABTS Chemical Properties,Usage,Production

Chemical Properties

Light green powder

Uses

Chromogenic substrate for peroxidase assays.2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt is widely utilized as a peroxidase substrate suitable for use in enzyme-linked immunosorbent assay (ELISA) procedures. It plays an important role in the food industry to measure the antioxidant capacities of foods. It is used to observe the reaction kinetics of specific enzymes and involved in glucose estimating reagent in blood serum.

Uses

  • 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS, has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).?
  • ABTS is also used to measure glucose concentrations in different solutions including blood serum.
  • The food industry uses ABTS to measure the antioxidant capacities within different foods.
  • Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results
  • It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Uses

Diammonium-2,2’Azino-bis(3-ethylbenzothiazoline-6-sulfonate) has been shown to inhibit catalase, a mammalian antioxidant enzyme which degrades hydrogen peroxide into water and oxygen species.

General Description

ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is water soluble chemical compound used as chromogenic substrate:

  • ABTS together with hydrogen peroxide for reaction with peroxidase enzymes (like HRP for example).
  • ABTS together with laccase or bilirubin oxidase.

ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).??? ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive.

ABTS Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from ABTS manufacturers

Hebei Chuanghai Biotechnology Co., Ltd
Product
Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) 30931-67-0
Price
US $10.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
10 mt
Release date
2024-12-11
Qiuxian Baitai New Material Co., LTD
Product
Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) 30931-67-0
Price
US $14.00/g
Min. Order
1KG
Purity
99%
Supply Ability
5ton/Month
Release date
2021-11-24
Zhuozhou Wenxi import and Export Co., Ltd
Product
Diammonium 2,2"-azino-bis(3-ethylbenzothiazoline-6-sulfonate) 30931-67-0
Price
US $15.00-10.00/KG
Min. Order
1KG
Purity
99%+ HPLC
Supply Ability
Monthly supply of 1 ton
Release date
2021-08-11

30931-67-0, ABTSRelated Search:


  • ABTS DIAMMONIUM SALT
  • ABTS-(NH4)2
  • ABTS(R), DIAMMONIUM SALT
  • ABTS SUBSTRATE
  • ABTS(TM) CHROMOPHORE, DIAMMONIUM SALT
  • ABTS
  • 2,2'-AZINOBIS(3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID AMMONIUM SALT)
  • 2,2'-AZINOBIS-(3 ETHYLBENZOTHIZOLINE-6-SULFONIC ACID) DIAMMONIUM SALT
  • 2,2'-AZINO-BIS(3-ETHYLBENZTHIAZOLINE-6-SULFONIC ACID), 2NH4
  • 2,2'-AZINOBIS(3-ETHYLBENZTHIAZOLINE-6-SULFONIC ACID) 2NH4-SALT
  • 2,2'-AZINO-BIS(3-ETHYLBENZTHIAZOLINE-6-SULFONIC ACID) DIAMMONIUM
  • 2,2'-AZINO-BIS(3-ETHYLBENZTHIAZOLINE-6-SULFONIC ACID) DIAMMONIUM SALT
  • 2,2'-AZINO-BIS(3-ETHYLBENZTHIOZOLINE-6-SULFONIC ACID) DIAMMONIUM SALT
  • 2,2'-AZINO-DI-[3-ETHYLBENZTHIAZO-LINE SULFONATE (6)] DIAMMONIUM SALT
  • 2,2'-AZINO-DI-(3-ETHYLBENZTHIAZOLINE SULFONIC ACID), DIAMMONIUM SALT
  • 2,2'-AZINO-DI-(3-ETHYLBENZTHIAZOLINE SULPHONIC ACID), DIAMMONIUM SALT
  • ABTS [=2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt)] [Spectrophotometric reagent for free chlorine and use as chromogenic substrate in enzyme-immunoassay]
  • 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid).2NH3
  • AzBTS-(NH4)2, Diammonium 2,2μ-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
  • 2,2'-Azinobis(2,3-dihydro-3-ethylbenzothiazole-6-sulfonic acid ammonium) salt
  • 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium
  • 2,2'-Azinobis[(2,3-dihydro-3-ethylbenzothiazole)-6-sulfonic acid ammonium] salt
  • ABTS (2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)diamm
  • ABTS [=2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt)] [for Biochemical Research]
  • 2,2''-Azino-bis(3-ethylbenzthiozoline-6-sulfonic acid) diamonium salt
  • ABTS [=2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt)]
  • 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt,AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
  • 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)
  • ABTS (2,2zzhlxy-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)diammonium salt)
  • 2,2'-azinobis(3-ethyl-2,3-dihydro-,diaMMoniuM salt
  • 2,2'-Azinobis(3-ehtylbenzothiazolin-6-sulfnic Acid)Diammonium Salt
  • [=2,2 -Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid AMMoniuM Salt)] [for BiocheMical Research]
  • 2,2’-azinobis(3-ethyl-2,3-dihydro-6-benzothiazolesulfonicacidiammonium
  • 2,2’-azinobis(3-ethyl-6-benzothiazolinesulfonicaciammoniumsalt(1:2)
  • 2,2’-azinobis[3-ethyl-2,3-dihydro-6-benzothiazolesulfonicacidiammoniumsa
  • 2,2’-azino-di(3-ethyl-benzthiazolinesulphonicacid(6)),ammoniumsalt
  • AZINO-BIS-3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID
  • 6-Benzothiazolesulfonic acid, 2,2-azinobis3-ethyl-2,3-dihydro-, diammonium salt
  • AzBTS-(NH4)2
  • diammonium 2,2'-azinobis[3-ethyl-2,3-dihydrobenzothiazole-6-sulphonate]
  • 2,2'-AZINO-BIS(3-ETHYLBENZTHIAZOLINE-6-S ULFONIC AC
  • ABTS-(NH4)2, PACKAGE WITH 50 TABLETS
  • 2,2'-AZINO-BIS(3-ETHYLBENZOTHIAZOLINE-6- SULFONIC ACID) DIAM
  • 2,2'-AZINODI(3-ETHYL BENZ-THIAZOLINE SULFONIC ACID) DIAMMONIUM SALT LIQUID SUBSTRATE IN ENZYME-IMMUNOASSAY
  • 2,2'-AZINODI(3-ETHYL BENZ-THIAZOLINE SULFONIC ACID) DIAMMONIUM SALT ULTRA PURE GRADE
  • DIAMMONIUM 2,2'-AZINO-BIS(3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID)
  • ABTS (2,2''-AZINO-BIS(3-ETHYLBENZTHIAZOLINE-6-SULFONIC ACID)DIAMMONIUM SALT)
  • ABTS&nbsp
  • ABTS substrate tablets
  • AzBTS-(NH4)2/ABTS diaMMoniuM salt
  • DiaMMoniuM 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS)
  • 6-Benzothiazolesulfonicacid, 2,2'-(1,2-hydrazinediylidene)bis[3-ethyl-2,3-dihydro-, aMMoniuM salt(1:2)
  • TRYPSIN 1:250 TISSUE CULTURE GRADE
  • Ammonium 2,2'-(hydrazine-1,2-diylidene)
  • bis(3-ethyl-2,3-dihydrobenzo[d]thiazole-6-sulfonate)
  • 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt≥ 98% (HPLC)
  • 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfoni
  • Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfote)