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MONOBROMOBIMANE

Product Name
MONOBROMOBIMANE
CAS No.
71418-44-5
Chemical Name
MONOBROMOBIMANE
Synonyms
MMB;MBBR;Nsc 608544;BROMOBIMANE;THIOLYTE(R) MB;MONOBROMOBIMANE;Bromobimane >THIOLYTE(R) MB REAGENT;MBBr [MonobroMobiMane];Special fluorescent reagent
CBNumber
CB0729365
Molecular Formula
C10H11BrN2O2
Formula Weight
271.11
MOL File
71418-44-5.mol
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MONOBROMOBIMANE Property

Melting point:
152-154 °C(lit.)
Boiling point:
327.8±44.0 °C(Predicted)
Density 
1.66±0.1 g/cm3(Predicted)
storage temp. 
2-8°C
solubility 
DMF: soluble
form 
powder
pka
-3.36±0.70(Predicted)
color 
Yellow
λmax
398 nm
BRN 
4430959
Stability:
Light Sensitive
Biological Applications
Glutathione S-transferase substrates; detecting glutathione S-transferase,thiols,sulfite,homocysteine,mycothiol,sulfur compounds; thiol-reactive probes
CAS DataBase Reference
71418-44-5
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Safety

Safety Statements 
22-24/25
WGK Germany 
3
8
HS Code 
2933.99.8290
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
596105
Product name
Bromobimane
Packaging
25mg
Price
$158
Updated
2024/03/01
Sigma-Aldrich
Product number
596105
Product name
Bromobimane
Packaging
100mg
Price
$491
Updated
2024/03/01
Sigma-Aldrich
Product number
69898
Product name
Bromobimane
Purity
BioReagent, suitable for fluorescence, ≥95% (HPCE)
Packaging
25MG
Price
$151
Updated
2023/06/20
Sigma-Aldrich
Product number
69898
Product name
Bromobimane
Purity
BioReagent, suitable for fluorescence, ≥95% (HPCE)
Packaging
100MG
Price
$484
Updated
2023/06/20
TCI Chemical
Product number
B4220
Product name
Bromobimane
Purity
>98.0%(HPLC)
Packaging
20mg
Price
$227
Updated
2024/03/01
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MONOBROMOBIMANE Chemical Properties,Usage,Production

Description

Monobromobimane is a thiol-reactive fluorogenic probe. It is cell-permeable, reacts rapidly at physiological pH with available thiol groups, and generates a stable fluorescent signal. Monobromobimane can be used to evaluate or quantify a variety of compounds containing reactive sulfur or thiol groups, including H2S, glutathione, proteins, and nucleotides. The absorption and emission maxima for monobromobimane are 398 and 490 nm, respectively.

Chemical Properties

Yellow Powder

Uses

Monobromobimane readily reacts with low molecular weight thiols. Bimanes are useful for detecting the distribution of protein thiols in cells before and after chemical reduction of disulfides. Fluorescence: max. Abs. 398nm; e x 10-3: 5.0

Uses

Monobromobimane is essentially nonfluorescent until conjugated to target molecule. It readily reacts with several low molecular weight thiols, including glutathione, mercaptopurine, peptides and plasma thiols, as well as with carboxylic acids.

Uses

double labeled product that can be fixed with aldehydes for cell-lineage tracing1, neuronal tracing2 and transplantation3.

Uses

Monobromobimane is a thiol-reactive fluorogenic probe. It is cell-permeable, reacts rapidly at physiological pH with available thiol groups, and generates a stable fluorescent signal. Monobromobimane can be used to evaluate or quantify a variety of compounds containing reactive sulfur or thiol groups, including H2S, glutathione, proteins, and nucleotides. The absorption and emission maxima for monobromobimane are 398 and 490 nm, respectively.

Definition

ChEBI: A pyrazolopyrazole that consists of 1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione bearing three methyl substituents at positions 2, 5 and 6 as well as a bromomethyl substituent at the 3-position.

MONOBROMOBIMANE Preparation Products And Raw materials

Raw materials

Preparation Products

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MONOBROMOBIMANE Suppliers

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025-58851786 17714337195
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71418-44-5, MONOBROMOBIMANERelated Search:


  • MBBR
  • MONOBROMOBIMANE
  • MMB
  • THIOLYTE MONOBROMOBIMANE REAGENT
  • THIOLYTE(R) MB
  • THIOLYTE(R) MB REAGENT
  • THIOLYTE(R) MONOBROMOBIMANE REAGENT
  • THIOLYTE(R) MONOBROMOBIMANE REAGENT, FLUORESCENT LABELING COMPOUND
  • MONOBROMOBIMANE, FOR FLUORESCENCE
  • BROMOBIMANE
  • 3-(broMoMethyl)-2,5,6-triMethylpyrazolo[1,2-a]pyrazole-1,7-dione
  • MBBr [MonobroMobiMane]
  • Nsc 608544
  • mBBr [Monobromobimane] *CAS 71418-44-5*
  • THIOLYTE Monobromobimane Reagent, Fluorescent Labeling Compound
  • Bromobimane &gt
  • 1H,7H-Pyrazolo[1,2-a]pyrazole-1,7-dione, 3-(bromomethyl)-2,5,6-trimethyl-
  • Special fluorescent reagent
  • 7-(bromomethyl)-1,2,6-trimethylpyrazolo[1,2-a]pyrazole-3,5-dione
  • 3-(bromomethyl)-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione
  • 71418-44-5
  • Detection
  • Fluorescent Probes, Labels, Particles and Stains
  • Biochemicals and Reagents
  • BioChemical
  • Pyrazole
  • Fluorescent Labels and Indicators
  • Fluorescent Labels & Indicators
  • MTS & Sulfhydryl Active Reagents