Evans Blue
- Product Name
- Evans Blue
- CAS No.
- 314-13-6
- Chemical Name
- Evans Blue
- Synonyms
- t1824;evablin;CI 23860;C.I.24075;DiazoBlue;blue Evan;Evans Blu;diazobleu;Evens Blue;EVANS BLUE
- CBNumber
- CB1161546
- Molecular Formula
- C34H29N6NaO14S4
- Formula Weight
- 896.86
- MOL File
- 314-13-6.mol
Evans Blue Property
- storage temp.
- Store at RT.
- solubility
- DMSO (Slightly), Water (Slightly)
- Colour Index
- 23860
- form
- Powder/Solid
- color
- Dark Brown
- Water Solubility
- 280 g/L (20 ºC)
- Merck
- 14,3905
- Stability:
- Stable. Incompatible with strong reducing agents, strong oxidizing agents.
- CAS DataBase Reference
- 314-13-6
- IARC
- 3 (Vol. 8, Sup 7) 1987
- EPA Substance Registry System
- C.I. Direct Blue 53 (314-13-6)
Safety
- Hazard Codes
- T,F
- Risk Statements
- 45-63-11-61-20/21/22
- Safety Statements
- 53-45-36/37-16-36/37/39
- WGK Germany
- 3
- RTECS
- QJ6440000
- TSCA
- Yes
- HS Code
- 32129000
- Toxicity
- LD50 intraperitoneal in mouse: 340mg/kg
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Danger
- Hazard statements
-
H350May cause cancer
- Precautionary statements
-
P201Obtain special instructions before use.
P202Do not handle until all safety precautions have been read and understood.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P308+P313IF exposed or concerned: Get medical advice/attention.
P405Store locked up.
P501Dispose of contents/container to..…
N-Bromosuccinimide Price
- Product number
- E2129
- Product name
- Evans Blue
- Purity
- Dye content ≥75%
- Packaging
- 10g
- Price
- $68
- Updated
- 2024/03/01
- Product number
- E2129
- Product name
- Evans Blue
- Purity
- Dye content ≥75%
- Packaging
- 50g
- Price
- $270
- Updated
- 2024/03/01
- Product number
- E0197
- Product name
- Evans Blue
- Purity
- >80.0%(HPLC)
- Packaging
- 25g
- Price
- $55
- Updated
- 2024/03/01
- Product number
- A16774
- Product name
- Evans Blue
- Packaging
- 10g
- Price
- $53.2
- Updated
- 2024/03/01
- Product number
- A16774
- Product name
- Evans Blue
- Packaging
- 50g
- Price
- $174
- Updated
- 2024/03/01
Evans Blue Chemical Properties,Usage,Production
Chemical Properties
Evans Blue is a blue crystal with a green metallic luster. It is very soluble in water, slightly soluble in ethanol, and almost insoluble in benzene, chloroform, and ether. It is soluble in acids and alkalis. It is hygroscopic, inducing moisture. It changes color when the pH is close to 10.
Uses
Evans Blue is used for estimation of blood volume and in double-labeling procedure for studying axonal branching. It acts as an inhibitor of L-glutamate and kainate receptor-mediated currents. It is also a P2X-selective purinoceptor antagonist. It is also useful in molecular biology.
Preparation
3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-1,3-disulfonic acid (2 Moore) coupled.
Definition
ChEBI: Evans blue is an organic sodium salt that is the tetrasodium salt of 6,6'-{(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis[diazene-2,1-diyl]}bis(4-amino-5-hydroxynaphthalene-1,3-disulfonate). It is sometimes used as a counterstain, especially in fluorescent methods to suppress background autofluorescence. It has a role as a histological dye, a fluorochrome, a teratogenic agent and a sodium channel blocker. It contains an Evans blue(4-).
General Description
Blue crystals with a greenish-bronze luster or a black powder.
Air & Water Reactions
Azo dyes can be explosive when suspended in air at specific concentrations. Soluble in water.
Reactivity Profile
Direct Blue 53 is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Direct Blue 53 can react with strong reducing and oxidizing agents.
Fire Hazard
Flash point data for Direct Blue 53 are unavailable; however, Direct Blue 53 is probably combustible.
Biological Activity
Potent inhibitor of L-glutamate uptake into synaptic vesicles. Also inhibits AMPA and kainate receptor-mediated currents (IC 50 values are 220 and 150 nM respectively). P2X-selective purinoceptor antagonist.
Safety Profile
Poison by intraperitoneal route.Moderately toxic by intravenous route. An experimentalteratogen. Other experimental reproductive effects.Questionable carcinogen with experimental tumorigenicdata. Mutation data reported. When heated todecomposition it
in vitro
evans blue inhibits the kainate-mediated responses of the non-nmda receptor subunits (giurl, g1ur1,2, g1url,3, and g1ur2,3) expressed in xenopus oocytes without the response of glur3 and g1ur6 at low concentrations (ic50= 355 nm for the subunit combination glur1,2). this pocess was partially reversible without competing with kainate for the agonist binding site [1]. in whole-cell patch clamp recordings of transfected human embryonic kidney 293 cells, evans blue is a potent inhibitor of glutamate-evoked currents mediated by the kainate-type receptor giur6 as long as the ampa-type receptor glurl. interestingly, pretreating with the lectin concanavalin cells recorded relatively little eb inhibition of giur6 currents, eliminating kainate receptor desensitization. in addition to decreasing glur6-mediated peak current amplitude, eb significantly changed receptor desensitization by slowing the rate of onset by ~2-fold (1 m eb) and the rate of recovery by ~2-fold (0.1 p.m eb), and enhancing the steady state to peak current amplitude ratio by ~50-fold (1 m eb) [2].
IC 50
220 and 150 nm for glurl and giur6, respectively.
storage
Room temperature
Staining Procedures
Evans Blue (3,7-Diamino-5-phenylphenazinium chloride) is used as a vital stain. It is used in a 0.05-0.25% aqueous solution for 5 minutes. Living cells remain unstained, while dead cells become blue.?
Properties and Applications
dark green light blue. Soluble in water for bright blue. The strong sulfuric acid for blue-ray green, diluted for light yellow. The dye solution to join hydrochloric acid as bright blue; Add sodium hydroxide solution for the purple and red.
| Standard | Acid Resistance | Alkali Resistance | Light Fastness | Soaping | Water | ||
| Fading | Stain | Fading | Stain | ||||
| ISO | 5 | 4-5 | 1-2 | 2 | 2 | ||
References
[1] roseth s, fykse em, fonnum f. uptake of l-glutamate into rat brain synaptic vesicles: effect of inhibitors that bind specifically to the glutamate transporter. j neurochem. 1995 jul;65(1):96-103.
[2] price cj, raymond la. evans blue antagonizes both alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate and kainate receptors and modulates receptor desensitization. mol pharmacol. 1996 dec; 50 (6):1665-71.
Evans Blue Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from Evans Blue manufacturers
- Product
- Direct Blue 53 314-13-6
- Price
- US $15.00-10.00/KG
- Min. Order
- 1KG
- Purity
- 99%+ HPLC
- Supply Ability
- Monthly supply of 1 ton
- Release date
- 2021-08-11
- Product
- Direct Blue 53 314-13-6
- Price
- US $1.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 100kg
- Release date
- 2019-07-09