TOTO 3

TOTO 3 Property

solubility 

Soluble in dimethyl sulfoxide

form 

powder

color 

Red

Appearance

blue solution

ex/em

642/660 nm

ε(extinction coefficient)

154100 cm−1M−1

Φ(quantum yield)

0.06

λmax

642nm (H₂O/DNA)

Major Application

Semiconductor devices

Biological Applications

Nucleic acid hybridization; detecting nucleic acids,cells,human papilloma virus (HPV),pathogens; counting embryoblasts; determining nuclease activity; retinal toxicity screening methods; studying cellular uptake of gene transfer complexes

Hazard and Precautionary Statements (GHS)

TOTO 3 Chemical Properties,Usage,Production

Description

TODi-3 (Thiazole Red Homodimer, also known as TOTO^®-3) is a far-red fluorescent carbocyanine dimeric dye. TODi-3 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a significant fluorescence enhancement upon binding to dsDNA.

The bright fluorescence signal and low background make TODi-3 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments. TODi-3 is non-cytotoxic and may be used for long-term monitoring of cell viability and dead cell detection in culture. The long-wavelength fluorescence of TODi-3 is well separated from green and red fluorophores, which makes it ideal for multicolor fluorescence labeling experiments.

Chemical Properties

ex/em : 642/660 nm
Extinction coefficient (ε) : 154100 cm??1M??1
Quantum yield (Φ) : 0.06

Uses

TOTO-3 (TOTO 3) is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.

References

[1] Sultana M, et al. A review on experimental chemically modified activated carbon to enhance dye and heavy metals adsorption[J]. Cleaner engineering and technology, 2022, 6: 100382.

Abs/Em Maxima

644/662 nm