ChemicalBook > CAS DataBase List > P-NITROPHENYL PHOSPHATE DI(TRIS) SALT

P-NITROPHENYL PHOSPHATE DI(TRIS) SALT

Product Name
P-NITROPHENYL PHOSPHATE DI(TRIS) SALT
CAS No.
68189-42-4
Chemical Name
P-NITROPHENYL PHOSPHATE DI(TRIS) SALT
Synonyms
PNPP-TRIS;PNPP, 2TRIS;PNPP TRIS SALT;PNP-DITRIS SALT;Einecs 269-198-6;P-NPP, DITRIS SALT;C6H6NO6P(C4H11NO3)2;l)-1,3-propanediol(1:2);P-NITROPHENYL PHOSPHATE DITRIS;4-NITROPHENYL PHOSPHATE DITRIS
CBNumber
CB1443187
Molecular Formula
C14H28N3O12P
Formula Weight
461.36
MOL File
68189-42-4.mol
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P-NITROPHENYL PHOSPHATE DI(TRIS) SALT Property

storage temp. 
2-8°C
solubility 
H2O: 0.1 g/mL, clear
form 
Off-white to pale yellow solid
color 
White to Orange to Green
Water Solubility 
water: soluble
BRN 
1578142
LogP
-2.16 at 22℃
CAS DataBase Reference
68189-42-4(CAS DataBase Reference)
EPA Substance Registry System
Mono(p-nitrophenyl) phosphate 2-amino-2-(hydroxymethyl)-1,3-propanediol salt (1:2) (68189-42-4)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36-24/25
WGK Germany 
3
F 
8-10-21
HS Code 
29199000
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
73737
Product name
4-Nitrophenyl phosphate bis(tris) salt
Purity
for the determination of phosphatase, ≥97.0% (enzymatic)
Packaging
5g
Price
$189
Updated
2025/07/31
Sigma-Aldrich
Product number
487655
Product name
p-Nitrophenyl Phosphate, DiTris Salt
Purity
Excellentsubstrateforalkalinephosphatase-basedELISAassays.
Packaging
5G
Price
$56.2
Updated
2025/07/31
TCI Chemical
Product number
N0422
Product name
4-Nitrophenyl Phosphate Di(tris) Salt Hydrate[Substrate for Phosphatase]
Purity
>90.0%(T)
Packaging
5g
Price
$82
Updated
2025/07/31
TCI Chemical
Product number
N0422
Product name
4-Nitrophenyl Phosphate Di(tris) Salt Hydrate[Substrate for Phosphatase]
Purity
>90.0%(T)
Packaging
25g
Price
$254
Updated
2022/04/27
Sigma-Aldrich
Product number
N3254
Product name
4-Nitrophenyl phosphate di(tris) salt
Purity
phosphatase substrate
Packaging
1g
Price
$58.2
Updated
2025/07/31
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P-NITROPHENYL PHOSPHATE DI(TRIS) SALT Chemical Properties,Usage,Production

Uses

4-Nitrophenyl phosphate di(tris) salt has been used:

  • as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates
  • to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)
  • for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)

Biological Activity

p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.

P-NITROPHENYL PHOSPHATE DI(TRIS) SALT Preparation Products And Raw materials

Raw materials

Preparation Products

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P-NITROPHENYL PHOSPHATE DI(TRIS) SALT Suppliers

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View Lastest Price from P-NITROPHENYL PHOSPHATE DI(TRIS) SALT manufacturers

Career Henan Chemical Co
Product
P-NITROPHENYL PHOSPHATE DI(TRIS) SALT 68189-42-4
Price
US $2.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
ask
Release date
2018-12-22

68189-42-4, P-NITROPHENYL PHOSPHATE DI(TRIS) SALTRelated Search:

Hydroxylamine hydrochloride Sodium isethionate Tris(hydroxymethyl)aminomethane 4-NITROPHENYL PHOSPHATE DI(TRIS) SALT,P-NITROPHENYL PHOSPHATE DI(TRIS) SALT Phenol diammonium 4-nitrophenyl phosphate P-NITROPHENYL PHOSPHATE TRISBUFFER SALT ANILINE PHOSPHATE, DIBASIC 2-Amino-2-methyl-1,3-propanediol TRIS(HYDROXYMETHYL)AMINOMETHANE PHOSPHATE P-NITROPHENYL PHOSPHATE DI(TRIS) SALT DI[TRIS(HYDROXYMETHYL)AMINOMETHANE] PHOSPHATE

  • p-nitrophenyl dihydrogen phosphate, compound with 2-amino-2-(hydroxymethyl)propane-1,3-diol (1:2)
  • p-Nitrophenylphosphate bis[tris-(hydroxymethylamino)-methane] salt
  • P-NITROPHENYL PHOSPHATE DITRIS
  • 4-NitrophenylphosphateBis(Tris)Salt
  • P-NITROPHENYL PHOSPHATE DI(TRIS) SALT
  • PNPP-TRIS
  • PNPP, 2TRIS
  • P-NPP, DITRIS SALT
  • l)-1,3-propanediol(1:2)
  • 4-NITROPHENYLPHOSPHORIC ACID BIS[TRIS(HYDROXYMETHYL)METHYLAMINE] SALT
  • 4-NITROPHENYLPHOSPHORIC ACID DI[TRIS(HYDROXYMETHYL)AMINOMETHANE] SALT
  • 4-NITROPHENYL PHOSPHATE BIS[TRIS(HYDROXYMETHYL)AMINOMETHANE] SALT
  • 4-NITROPHENYL PHOSPHATE DITRIS
  • 4-NITROPHENYL PHOSPHATE DI(TRIS) SALT
  • 4-nitrophenylphosphoric acid bis[tris(hydroxymethyl)aminomethane] salt
  • PNP-DITRIS SALT
  • 4-Nitrophenylphosphoric Acid Bis[tris(hydroxymethyl)aminomethane] Salt Hydrate [Substrate for Phosphatase]
  • 4-Nitrophenyl phosphate bistris(hydroxymethyl)aminomethaneü salt, 98%
  • p-NITROPHENYLPHOSPHATE DITRIS SALT crystalline extrapure
  • 4-nitrophenyl phosphate (hydroxymethyl)aminomethane salt
  • 4-Nitrophenylphosphoric Acid Bis[tris(hydroxymethyl)aminomethane] Salt Hydrate
  • C6H6NO6P(C4H11NO3)2
  • Einecs 269-198-6
  • Mono(p-nitrophenyl) phosphate 2-amino-2-(hydroxymethyl)-1,3-propanediol salt (1:2)
  • Phosphoric acid, mono(4-nitrophenyl) ester, compd. with 2-amino-2-(hydroxymethyl)-1,3-propanediol (1:2)
  • p-Nitrophenyl phosphate di(tris(hydroxymethyl)methylamine) salt
  • p-Nitrophenyl phosphate bis[tri(hydroxymethyl)aminomethane) salt
  • 4-Nitrophenyl phosphate bis(tris(hydroxymethyl)amino methane)
  • 4-Nitrophenyl Phosphate Di(tris) Salt Hydrate [Substrate for Phosphatase]
  • Phosphoric Acid 4-Nitrophenyl Ester Bis[tris(hydroxymethyl)aminomethane] Salt 4-Nitrophenylphosphoric Acid Bis[tris(hydroxymethyl)aminomethane] Salt 4-Nitrophenyl Phosphate Bis(tris) Salt
  • 4-Nitrophenyl Phosphate Di(tris) Salt Hydrate [Substrate for Phosphatase], >90.0%(T)
  • 4-NitrophenylPhosphateDi(tris)SaltHydrate[SubstrateforPhosphatase]&gt
  • Phosphoric Acid 4-Nitrophenyl Ester Bis[tris(hydroxymethyl)aminomethane] Salt
  • 4-Nitrophenyl Phosphate Di(tris) Salt Hydrate
  • 4-Nitrophenyl Phosphate Di(tris) Salt [Substrate for Phosphatase]
  • p-Nitrophenylphosphate Ditris Salt crystalline (PNPP-d) extrapure, 99%
  • 4-Nitrophenyl phosphate di(tris(hydroxymethyl)methylamine) salt
  • 4-Nitrophenyl phosphate (ditromethamine)
  • PNPP TRIS SALT
  • 4-Nitrophenyl phosphate di(tris) salt phosphatase substrate
  • <i>p</i>-Nitrophenyl Phosphate, DiTris Salt
  • 68189-42-4
  • C6H6NO6P2C4H11NO3
  • C6H6NO6PC4H11NO32xH2O
  • C14H28N3O12P
  • Substrates by Enzyme
  • Enzyme Substrates
  • Enzymes, Inhibitors, and Substrates
  • Biochemicals and Reagents
  • BioChemical
  • substrate
  • Alkaline Phosphatase
  • Alkaline PhosphataseEnzyme Substrates
  • Alphabetical ListingELISA Substrates
  • Detection Substrates
  • Enzyme Substrates
  • Powders
  • Substrates by Enzyme