description Chemical Properties Uses of Biology Uses
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Tris(hydroxymethyl)aminomethane

description Chemical Properties Uses of Biology Uses
Product Name
Tris(hydroxymethyl)aminomethane
CAS No.
77-86-1
Chemical Name
Tris(hydroxymethyl)aminomethane
Synonyms
TRIS;TROMETHAMINE;TROMETAMOL;TRIS(HYDROXYMETHYL)AMINOMETHANE;2-AMINO-2-(HYDROXYMETHYL)PROPANE-1,3-DIOL;TRIS BUFFER;TRIZMA BASE;Trizma;TRIS AMINO;THAM
CBNumber
CB8336488
Molecular Formula
C4H11NO3
Formula Weight
121.14
MOL File
77-86-1.mol
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Tris(hydroxymethyl)aminomethane Property

Melting point:
167-172 °C(lit.)
Boiling point:
219-220 °C10 mm Hg(lit.)
Density 
1,353 g/cm3
vapor pressure 
0.0267 hPa (20 °C)
refractive index 
1.4170 (estimate)
Flash point:
219-220°C/10mm
storage temp. 
20-25°C
solubility 
H2O: 4 M at 20 °C, clear, colorless
form 
crystalline
color 
white
PH
10.5-12.0(4 m in water, 25 °C)
pka
8.1(at 25℃)
PH Range
7 - 9
Water Solubility 
550 g/L (25 ºC)
Sensitive 
Hygroscopic
λmax
λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.08
Merck 
14,9772
BRN 
741883
Stability:
Stable. Incompatible with bases, strong oxidizing agents. Protect from moisture.
InChIKey
LENZDBCJOHFCAS-UHFFFAOYSA-N
CAS DataBase Reference
77-86-1(CAS DataBase Reference)
NIST Chemistry Reference
1,3-Propanediol, 2-amino-2-(hydroxymethyl)-(77-86-1)
EPA Substance Registry System
Tris(hydroxymethyl)aminomethane (77-86-1)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36-37/39-24/25-23
WGK Germany 
2
RTECS 
TY2900000
3
TSCA 
Yes
HazardClass 
IRRITANT
HS Code 
29221980
Hazardous Substances Data
77-86-1(Hazardous Substances Data)
Toxicity
LD50 orally in Rabbit: > 5000 mg/kg LD50 dermal Rat > 5000 mg/kg
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
TRIS-RO
Product name
Tris base
Packaging
1kg
Price
$221
Updated
2023/01/07
Sigma-Aldrich
Product number
TRIS-RO
Product name
Tris base
Packaging
5kg
Price
$497
Updated
2023/01/07
Sigma-Aldrich
Product number
T6066
Product name
Trizma® base
Purity
BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
Packaging
100g
Price
$55.4
Updated
2023/01/07
Sigma-Aldrich
Product number
T6066
Product name
Trizma® base
Purity
BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
Packaging
500g
Price
$120
Updated
2023/01/07
Sigma-Aldrich
Product number
T6066
Product name
Trizma® base
Purity
BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
Packaging
1KT
Price
$194
Updated
2023/01/07
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Tris(hydroxymethyl)aminomethane Chemical Properties,Usage,Production

description

Tris, or tris(hydroxymethyl)aminomethane, or known during medical use as tromethamine or THAM, is an organic compound with the formula (HOCH2)3CNH2. It is extensively used in biochemistry and molecular biology as a component of buffer solutions such as in TAE and TBE buffers, especially for solutions of nucleic acids. It contains a primary amine and thus undergoes the reactions associated with typical amines, e.g. condensations with aldehydes. Tris also complexes with metal ions in solution. In medicine, tromethamine is occasionally used as a drug, given in intensive care for its properties as a buffer for the treatment of severe metabolic acidosis in specific circumstances. Some medications are formulated as the "tromethamine salt" including hemabate (carboprost as trometamol salt), and "ketorolac trometamol".

Chemical Properties

White crystal or powder. Melting point 171-172℃, boiling point 219-220℃/1.3kPa, soluble in ethanol and water, slightly soluble in ethyl acetate, benzene, insoluble in ether, carbon tetrachloride,copper,aluminum corrosion effects,irritant.

Uses of Biology

Tris base, also known as THAM, is widely used as a biological buffer or as a component of buffer formulations such as TAE and TBE buffers. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.

Uses

1, Tris buffer, only nucleic acids and proteins are widely used as solvents.
2, Tris is used for protein crystal growth under different pH conditions.
3, Tris buffer having a low ionic strength can be used nematode (C. elegans) is formed lamin (lamin) intermediate fibers.
4, Tris is a major component of protein electrophoresis buffer.
5, Tris for the preparation of a surfactant, a vulcanization accelerator, and some drug intermediates.
6, Tris titrated also used as standard.

Chemical Properties

White, crystalline solid. PH (0.1M aqueous solution) 10.6, corrosive to copper, brass, aluminum. Solubility in water 80 g/100 cc (20C). Combustible.

Originator

Trisaminol,Bellon,France,1964

Uses

tromethamine can help mask odor in a formulation. It is also used as a buffer, keeping product pH stable.

Uses

In the synthesis of surface-active agents, vulcanization accelerators, pharmaceuticals. As emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, leather dressings, textile specialties, polishes, cleaning Compounds, so-called soluble oils. Absorbent for acidic gases. Biological buffer.

Uses

Tris base has been used:

  • In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
  • As a component of sample buffer for SDS-PAGE
  • In the preparation of IEC (ion-exchange chromatography) buffer

Application

Tris(hydroxymethyl)aminomethane is mainly required for preparation of buffers at physiological range of 7.3 to 7.5. The prepared buffers are compatible with biological fluids. It is of importance to laboratories as a standard pH solution.
Tris(hydroxymethyl)aminomethane has been used as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.
Tris(hydroxymethyl)aminomethane is used as a buffer in biochemistry and molecular biology laboratories. It is used as a primary standard to standardize acid solutions for chemical analysis. It finds application in cell membranes to increase permeability. As an alternative to sodium bicarbonate, it used in the treatment of metabolic acidosis. It acts as a precursor for the preparation of polymers, oxazolones and oxazolidines.

Preparation

Tris is prepared industrially by the exhaustive condensation of nitromethane with formaldehyde under basic conditions (i.e. repeated Henry reactions) to produce the intermediate (HOCH2)3CNO2, which is subsequently hydrogenated to give the final product.

Manufacturing Process

Nitromethane is reacted with formaldehyde to give tris(hydroxymethyl)nitromethane in an initial step. This intermediate may be reduced by catalytic hydrogenation (US Patent 2,174,242) or by electrolytic reduction (US Patent 2,485,982).

brand name

Tham (Hospira).

Therapeutic Function

Antacid

General Description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Biochem/physiol Actions

Trizma base, commonly known as tris is widely used as a biological buffer or as a component of buffer formulations like- Tris-EDTA (TE) buffer, Tris magnesium buffer or Tris-acetate-EDTA (TAE) buffer. It enhances the membrane permeability of cell membranes.

Purification Methods

TRIS can ordinarily be obtained in highly pure form suitable for use as an acidimetric standard. If only impure material is available, it should be crystallised from 20% EtOH, aqueous MeOH (m 171.1o) or isopropanol (m 172-173o). Dry it in a vacuum desiccator over P2O5 or CaCl2. Alternatively, it is dissolved in twice its weight of water at 55-60o, filtered, concentrated to half its volume and poured slowly, with stirring, into about twice its volume of EtOH. The crystals which separate on cooling to 3-4o are filtered off, washed with a little MeOH, air dried by suction, then finally ground and dried in a vacuum desiccator over P2O5. It has also been recrystallised from water, MeOH or aqueous MeOH, and vacuum dried at 80o for 2 days. [Beilstein 4 H 303, 4 III 857, 4 IV 1903.]

Tris(hydroxymethyl)aminomethane Preparation Products And Raw materials

Raw materials

Preparation Products

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Tris(hydroxymethyl)aminomethane Suppliers

Hubei New Desheng Materials Technology Co., Ltd.
Tel
18971041571
Email
vickyzhao@whdschem.com
Country
China
ProdList
57
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58
Shangqiu tengfei biotechnology co., ltd
Tel
0370-6331388 16637075318
Email
1303279042@qq.com
Country
China
ProdList
1
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58
Shanghai Jizhi Biochemical Technology Co. Ltd.
Tel
400-400-9004166 18017137653
Email
3007522970@qq.com
Country
China
ProdList
52647
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58
Spectrum Chemical Manufacturing Corp.
Tel
021-67601398-809 15221380277
Fax
021-57711696
Email
marketing_china@spectrumchemical.com
Country
China
ProdList
9666
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60
Shanghai Aladdin Bio-Chem Technology Co.,LTD
Tel
400-62063333-1 15601730970
Fax
021-50323701
Email
market@aladdin-e.com
Country
China
ProdList
24968
Advantage
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Nanjing Search Biotech Co., Ltd
Tel
+86-025-81682922 +86-13515111588
Fax
025-85860962
Email
sales@searchbio.com.cn
Country
China
ProdList
130
Advantage
68
Shanghai Uteam Biotechnology Co., Ltd.
Tel
021-65211385-102 13311776681
Email
3338195766@QQ.com
Country
China
ProdList
5169
Advantage
55
Dongyang Baihang Chemical Co., Ltd.
Tel
0579-86886233 18006543966
Fax
QQ:355093164
Email
355093164@qq.com
Country
China
ProdList
97
Advantage
58
Yantai Xianhua Chemical Technology Co., Ltd (HSBC Shandong Yantai Fine Chemical Co., Ltd.)
Tel
18754503080 18754503080
Email
sunzhulin@xianhualc.com
Country
China
ProdList
99
Advantage
58
Shandong baiman biology science and technology co., ltd
Tel
19953048686
Email
1580153941@qq.com
Country
China
ProdList
3
Advantage
58
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View Lastest Price from Tris(hydroxymethyl)aminomethane manufacturers

Hebei Crovell Biotech Co Ltd
Product
Tris Base 77-86-1
Price
US $6.00/Kg/Bag
Min. Order
1g
Purity
99%
Supply Ability
10000kg
Release date
2021-07-01
Hebei Guanlang Biotechnology Co., Ltd.
Product
Trometamol 77-86-1
Price
US $30.00-10.00/KG
Min. Order
50KG
Purity
99%
Supply Ability
500000kg
Release date
2022-09-22
Jinan Finer Chemical Co., Ltd
Product
Trometamol 77-86-1
Price
US $0.00/Kg/Drum
Min. Order
1KG
Purity
99%
Supply Ability
200mt
Release date
2020-11-11

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