TRIS hydrochloride
- Product Name
- TRIS hydrochloride
- CAS No.
- 1185-53-1
- Chemical Name
- TRIS hydrochloride
- Synonyms
- TRIS-HCL;Tris-HCI;TRIZMA HYDROCHLORIDE;TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE;TRIS-HCL BUFFER;TROMETHAMINE HYDROCHLORIDE;Tris hydrochloric acid;TRIZMA HYDROCHLORIDE REAGENT GRADE;TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE;2-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride
- CBNumber
- CB2677770
- Molecular Formula
- C4H12ClNO3
- Formula Weight
- 157.6
- MOL File
- 1185-53-1.mol
TRIS hydrochloride Property
- Melting point:
- 150-152 °C
- Boiling point:
- 225℃[at 101 325 Pa]
- Density
- 1.05 g/mL at 20 °C
- vapor pressure
- 0Pa at 20℃
- storage temp.
- room temp
- solubility
- H2O: 4 M at 20 °C, clear, colorless
- form
- crystalline
- color
- clear colorless (40 % (w/w) in H2O) solution
- PH Range
- 7.0 - 9.0
- PH
- 2.5-4.0 (25℃, 4M in H2O)
- pka
- 8.1(at 25℃)
- Water Solubility
- SOLUBLE
- Sensitive
- Hygroscopic
- λmax
- λ: 260 nm Amax: 0.12
λ: 280 nm Amax: 0.08 - BRN
- 3675235
- Stability:
- Stable. Incompatible with strong oxidizing agents, bases, moisture.
- InChIKey
- QKNYBSVHEMOAJP-UHFFFAOYSA-N
- LogP
- -3.6 at 20℃
- CAS DataBase Reference
- 1185-53-1(CAS DataBase Reference)
- EPA Substance Registry System
- 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1185-53-1)
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
- Precautionary statements
-
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- T3253
- Product name
- Trizma® hydrochloride
- Purity
- reagent grade, ≥99.0% (titration), crystalline
- Packaging
- 50KG
- Price
- $7730
- Updated
- 2024/03/01
- Product number
- T15760
- Product name
- Trizma® hydrochloride
- Purity
- reagent grade, ≥99%, crystalline
- Packaging
- 500G
- Price
- $113
- Updated
- 2024/03/01
- Product number
- T15760
- Product name
- Trizma® hydrochloride
- Purity
- reagent grade, ≥99%, crystalline
- Packaging
- 1KG
- Price
- $210
- Updated
- 2024/03/01
- Product number
- T15760
- Product name
- Trizma® hydrochloride
- Purity
- reagent grade, ≥99%, crystalline
- Packaging
- 5KG
- Price
- $895
- Updated
- 2024/03/01
- Product number
- T15760
- Product name
- Trizma® hydrochloride
- Purity
- reagent grade, ≥99%, crystalline
- Packaging
- 10KG
- Price
- $1520
- Updated
- 2024/03/01
TRIS hydrochloride Chemical Properties,Usage,Production
Description
Abbreviated as Tris HCl (TRIS hydrochloride), Tris (hydroxymethyl) aminomethane hydrochloride is an organic compound commonly utilized in buffer solutions such as TAE or TBE for electrophoresis gels. It boasts high solubility in water and offers utility within the pH range of 7.0-9.0. The compound is instrumental in preparing Laemmli buffer, one of the most widely used SDS-PAGE buffers, and also finds application in various custom running and loading buffers, frequently in combination with glycine and SDS. Lysis, the initial step of DNA extraction, involves a buffer containing Tris and EDTA (ethylenediaminetetraacetic acid). In this process, EDTA binds to divalent cations such as calcium and magnesium. Due to their role in maintaining cell membrane integrity, their removal by EDTA leads to membrane destabilization. Tris plays a key role as the primary buffering component, maintaining the pH of the buffer at a stable point, typically around 8.0. Additionally, Tris may interact with the LPS (lipopolysaccharide) in the membrane, contributing to further membrane destabilization. This underscores Tris HCl's significance in various laboratory procedures.
Chemical Properties
TRIS hydrochloride is a white crystalline powder that has a pKa of 8.08 at 25°C and pH range of 7.0 to 9.0 that coincides with the physiological pH of most living organisms because of which it is commonly used as a component of buffer solutions in biology, biochemistry and molecular biology applications. Tris salts are also used for crystallization of proteins at various pH values. Neither Tris base nor Tris hydrochloride by itself provide adequate buffering capacity. Generally the two need to be mixed together to provide a buffer with pH between 7 and 9 to provide adequate buffering.
Uses
Tris Hydrochloride is a buffering medium for electrophoresis, molecular biology, and cell culture applications. It may be combined with Tris Base to simplify Tris buffer preparation. Tris Hydrochloride has been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs. In immunohistochemistry, Tris Hydrochloride has also been used with urea as a method for antigen retrieval.
Uses
TRIS hydrochloride is a high quality buffer component used in SDS-PAGE. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. It is also used in the robust generation of transgenic mice.
Application
TRIS hydrochloride is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.
Preparation
The preparation of TRIS hydrochloride is as follows:After reacting 2-amino-1,3-propanediol (2.50 g, 27.44 mmol) and formic acid (1.26 g, 27.44 mmol) in 50 ml of water at room temperature for 3 hours, water was distilled off under reduced pressure, A liquid was obtained. By washing the obtained liquid,An ammonium formate salt (3.76 g, 27.44 mmol) was obtained as a colorless transparent liquid.
General Description
TRIS hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.
Biological Activity
Commonly used laboratory reagent
Purification Methods
Crystallise the salt from 50% EtOH, then from 70% EtOH. TRIS-hydrochloride is also available commercially in a highly pure state. Otherwise, recrystallise it from 50% EtOH, then 70% EtOH, and dry it below 40o to avoid risk of decomposition. [Beilstein 4 H 304.]
TRIS hydrochloride Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from TRIS hydrochloride manufacturers
- Product
- TRIS hydrochloride 1185-53-1
- Price
- US $25.00-20.00/kg
- Min. Order
- 1kg
- Purity
- 99%
- Supply Ability
- 8000kg
- Release date
- 2023-09-15
- Product
- Trometamol hydrochloride 1185-53-1
- Price
- US $0.98/g
- Min. Order
- 1g
- Purity
- 99%
- Supply Ability
- 2000
- Release date
- 2024-06-05
- Product
- TRIS hydrochloride 1185-53-1
- Price
- US $10.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 10 mt
- Release date
- 2021-05-13