Tris(hydroxymethyl)aminomethane

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Tris(hydroxymethyl)aminomethane

description Chemical Properties Uses of Biology Uses

Product Name: Tris(hydroxymethyl)aminomethane
CAS No.: 77-86-1
Chemical Name: Tris(hydroxymethyl)aminomethane
Synonyms: TRIS;TROMETHAMINE;TROMETAMOL;TRIS(HYDROXYMETHYL)AMINOMETHANE;2-AMINO-2-(HYDROXYMETHYL)PROPANE-1,3-DIOL;TRIS BUFFER;TRIZMA BASE;Trizma;TRIS AMINO;THAM
CBNumber: CB8336488
Molecular Formula: C4H11NO3
Formula Weight: 121.14
MOL File: 77-86-1.mol

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Tris(hydroxymethyl)aminomethane Property

Melting point:: 167-172 °C(lit.)
Boiling point:: 219-220 °C10 mm Hg(lit.)
Density: 1,353 g/cm³
vapor pressure: 0.0267 hPa (20 °C)
refractive index: 1.4170 (estimate)
Flash point:: 219-220°C/10mm
storage temp.: 20-25°C
solubility: H₂O: 4 M at 20 °C, clear, colorless
form: crystalline
color: white
PH: 10.5-12.0(4 m in water, 25 °C)
pka: 8.1(at 25℃)
PH Range: 7 - 9
Water Solubility: 550 g/L (25 ºC)
Sensitive: Hygroscopic
λmax: λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.08
Merck: 14,9772
BRN: 741883
Stability:: Stable. Incompatible with bases, strong oxidizing agents. Protect from moisture.
InChIKey: LENZDBCJOHFCAS-UHFFFAOYSA-N
LogP: -2.515 (est)
CAS DataBase Reference: 77-86-1(CAS DataBase Reference)
NIST Chemistry Reference: 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-(77-86-1)
EPA Substance Registry System: Tris(hydroxymethyl)aminomethane (77-86-1)

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Safety

Hazard Codes: Xi
Risk Statements: 36/37/38
Safety Statements: 26-36-37/39-24/25-23
WGK Germany: 2
RTECS: TY2900000
F: 3
TSCA: Yes
HazardClass: IRRITANT
HS Code: 29221980
Hazardous Substances Data: 77-86-1(Hazardous Substances Data)
Toxicity: LD50 orally in Rabbit: > 5000 mg/kg LD50 dermal Rat > 5000 mg/kg

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: TRIS-RO
Product name: Tris base
Packaging: 1kg
Price: $234
Updated: 2024/03/01

Sigma-Aldrich

Product number: TRIS-RO
Product name: Tris base
Packaging: 5kg
Price: $527
Updated: 2024/03/01

Sigma-Aldrich

Product number: T6066
Product name: Trizma® base
Purity: BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
Packaging: 100g
Price: $57.6
Updated: 2024/03/01

Sigma-Aldrich

Product number: T6066
Product name: Trizma® base
Purity: BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
Packaging: 500g
Price: $125
Updated: 2024/03/01

Sigma-Aldrich

Product number: T6066
Product name: Trizma® base
Purity: BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
Packaging: 1kg
Price: $214
Updated: 2024/03/01

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Tris(hydroxymethyl)aminomethane Chemical Properties,Usage,Production

description

Tris, or tris(hydroxymethyl)aminomethane, or known during medical use as tromethamine or THAM, is an organic compound with the formula (HOCH2)3CNH2. It is extensively used in biochemistry and molecular biology as a component of buffer solutions such as in TAE and TBE buffers, especially for solutions of nucleic acids. It contains a primary amine and thus undergoes the reactions associated with typical amines, e.g. condensations with aldehydes. Tris also complexes with metal ions in solution. In medicine, tromethamine is occasionally used as a drug, given in intensive care for its properties as a buffer for the treatment of severe metabolic acidosis in specific circumstances. Some medications are formulated as the "tromethamine salt" including hemabate (carboprost as trometamol salt), and "ketorolac trometamol".

Chemical Properties

Tris(hydroxymethyl)aminomethane is a white crystal or powder. The melting point of Tris(hydroxymethyl)aminomethane is 171-172℃ and the boiling point of Tris is 219-220℃/1.3kPa. It is soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, and insoluble in ether and carbon tetrachloride. It has a corrosive effect on copper and aluminum and has strong irritation.

Uses of Biology

Tris base, also known as THAM, is widely used as a biological buffer or as a component of buffer formulations such as TAE and TBE buffers. Tris has a pK_a of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.

Uses

1, Tris buffer, only nucleic acids and proteins are widely used as solvents.
2, Tris is used for protein crystal growth under different pH conditions.
3, Tris buffer having a low ionic strength can be used nematode (C. elegans) is formed lamin (lamin) intermediate fibers.
4, Tris is a major component of protein electrophoresis buffer.
5, Tris for the preparation of a surfactant, a vulcanization accelerator, and some drug intermediates.
6, Tris titrated also used as standard.

Chemical Properties

White, crystalline solid. PH (0.1M aqueous solution) 10.6, corrosive to copper, brass, aluminum. Solubility in water 80 g/100 cc (20C). Combustible.

Originator

Trisaminol,Bellon,France,1964

Uses

tromethamine can help mask odor in a formulation. It is also used as a buffer, keeping product pH stable.

Uses

Tris base has been used:

In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
As a component of sample buffer for SDS-PAGE
In the preparation of IEC (ion-exchange chromatography) buffer

Uses

Tris(hydroxymethyl)aminomethane could used in the synthesis of surface-active agents, vulcanization accelerators, pharmaceuticals. As emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, leather dressings, textile specialties, polishes, cleaning Compounds, so-called soluble oils. Absorbent for acidic gases. Biological buffer.

Preparation

Tris(hydroxymethyl)aminomethane is prepared industrially by the exhaustive condensation of nitromethane with formaldehyde under basic conditions (i.e. repeated Henry reactions) to produce the intermediate (HOCH2)3CNO2, which is subsequently hydrogenated to give the final product.

Definition

ChEBI: Tris is a primary amino compound that is tert-butylamine in which one hydrogen attached to each methyl group is replaced by a hydroxy group. A compound widely used as a biological buffer substance in the pH range 7--9; pKa = 8.3 at 20 ℃; pKa = 7.82 at 37 ℃. It has a role as a buffer. It is a triol and a primary amino compound. It is a conjugate base of a member of Htris.

Application

Tris(hydroxymethyl)aminomethane is mainly required for preparation of buffers at physiological range of 7.3 to 7.5. The prepared buffers are compatible with biological fluids. It is of importance to laboratories as a standard pH solution.
Tris(hydroxymethyl)aminomethane has been used as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.
Tris(hydroxymethyl)aminomethane is used as a buffer in biochemistry and molecular biology laboratories. It is used as a primary standard to standardize acid solutions for chemical analysis. It finds application in cell membranes to increase permeability. As an alternative to sodium bicarbonate, it used in the treatment of metabolic acidosis. It acts as a precursor for the preparation of polymers, oxazolones and oxazolidines.

Manufacturing Process

Nitromethane is reacted with formaldehyde to give tris(hydroxymethyl)nitromethane in an initial step. This intermediate may be reduced by catalytic hydrogenation (US Patent 2,174,242) or by electrolytic reduction (US Patent 2,485,982).

brand name

Tham (Hospira).

Therapeutic Function

Antacid

General Description

Tris(hydroxymethyl)aminomethane is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Biochem/physiol Actions

Trizma base, commonly known as tris(hydroxymethyl)aminomethane is widely used as a biological buffer or as a component of buffer formulations like- Tris-EDTA (TE) buffer, Tris magnesium buffer or Tris-acetate-EDTA (TAE) buffer. It enhances the membrane permeability of cell membranes.

storage

Store at RT

Purification Methods

Tris(hydroxymethyl)aminomethane can ordinarily be obtained in highly pure form suitable for use as an acidimetric standard. If only impure material is available, it should be crystallised from 20% EtOH, aqueous MeOH (m 171.1o) or isopropanol (m 172-173o). Dry it in a vacuum desiccator over P2O5 or CaCl2. Alternatively, it is dissolved in twice its weight of water at 55-60o, filtered, concentrated to half its volume and poured slowly, with stirring, into about twice its volume of EtOH. The crystals which separate on cooling to 3-4o are filtered off, washed with a little MeOH, air dried by suction, then finally ground and dried in a vacuum desiccator over P2O5. It has also been recrystallised from water, MeOH or aqueous MeOH, and vacuum dried at 80o for 2 days. [Beilstein 4 H 303, 4 III 857, 4 IV 1903.]

Tris(hydroxymethyl)aminomethane Preparation Products And Raw materials

Raw materials

Nitromethane Potassium carbonate Paraformaldehyde Palladium Methanol Tris(hydroxymethyl)nitromethane

Preparation Products

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View Lastest Price from Tris(hydroxymethyl)aminomethane manufacturers

Hebei Longbang Technology Co., Ltd

Product: Tris Base 77-86-1
Price: US $6.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 20TONS
Release date: 2024-05-10

Jinan Finer Chemical Co., Ltd

Product: Trometamol 77-86-1
Price: US $0.00/Kg/Drum
Min. Order: 1KG
Purity: 99%
Supply Ability: 200mt
Release date: 2020-11-11

Sinoway Industrial co., ltd.

Product: Trometamol 77-86-1
Price: US $0.00-0.00/Kg/Bag
Min. Order: 1Kg/Bag
Purity: 0.99
Supply Ability: 20 tons
Release date: 2024-06-27

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