N-(1-ANILINONAPHTHYL-4)-MALEIMIDE

N-(1-ANILINONAPHTHYL-4)-MALEIMIDE Property

Melting point:

207 °C

Boiling point:

454.02°C (rough estimate)

Density 

1.2303 (rough estimate)

refractive index 

1.5700 (estimate)

pka

3.50±0.30(Predicted)

CAS DataBase Reference

50539-45-2

Safety

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36

WGK Germany 

3

F 

8-10-21

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P321Specific treatment (see … on this label).

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P362Take off contaminated clothing and wash before reuse.

N-(1-ANILINONAPHTHYL-4)-MALEIMIDE Chemical Properties,Usage,Production

Chemical Properties

red powder

Uses

N-(4-Anilino-1-naphthyl)maleimide is a fluorescent probe that can be used to detect thiol groups in proteins. N-(4-Anilino-1-naphthyl)maleimide reacts with thiol groups and immediately produces a stable fluorescent compound with the λex=355 nm, λem=448 nm[1].

References

[1] T Honda, et al. Characterization of the reactivity of sulphydryl groups in tryptophanase by a dual-monitoring high-performance liquid chromatographic system with a site-directed fluorescent reagent. J Chromatogr. 1986 Dec 26;371:353-60. DOI:10.1016/s0021-9673(01)94718-1
[2] S Chaen, et al. Fluorescence properties and contraction characteristics of ANM (N-(1-anilinonaphthyl-4)maleimide)-labeled rabbit psoas muscle fibers. J Biochem. 1985 Oct;98(4):939-47. DOI:10.1093/oxfordjournals.jbchem.a135374