5'(6)-FAM

Product Name: 5'(6)-FAM
CAS No.: 204697-37-0
Chemical Name: 5'(6)-FAM
Synonyms: 5'(6)-FAM;5'-(6-FAM) CEP;5'-FAM Amidite;5'-Fluorescein CEP;FAM-Phosphoramidite;6-FAM-phosphoramidite;6-Fluorescein amidite;5'-6-FAM-PhosphoraMidite;6-FAM CE Phosphoramidite;Fluorescein Phosphoramidite
CBNumber: CB1726477
Molecular Formula: C46H58N3O10P
Formula Weight: 843.94
MOL File: 204697-37-0.mol

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5'(6)-FAM Property

Boiling point:: 850.6±65.0 °C(Predicted)
storage temp.: -20°C
solubility: acetonitrile: soluble 0.1M, clear, colorless to faintly yellow (liquid)
pka: 14.56±0.20(Predicted)
color: solid white to off-white
Appearance: off-white solid
InChIKey: MGPYJVWEJNTXLC-UHFFFAOYSA-N
SMILES: C12(C3=C(C=C(OC(=O)C(C)(C)C)C=C3)OC3=C1C=CC(OC(=O)C(C)(C)C)=C3)C1=C(C=CC(C(=O)NCCCCCCOP(N(C(C)C)C(C)C)OCCC#N)=C1)C(=O)O2

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Safety

WGK Germany: 3

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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: M041130
Product name: 6-Fluorescein Phosphoramidite
Purity: configured for ABI
Packaging: 0.1g
Price: $135
Updated: 2025/07/31

Sigma-Aldrich

Product number: M041130
Product name: 6-Fluorescein Phosphoramidite
Purity: configured for ABI
Packaging: 0.25g
Price: $570
Updated: 2025/07/31

Sigma-Aldrich

Product number: M041180
Product name: 6-Fluorescein Phosphoramidite
Purity: configured for PerkinElmer, configured for Polygen
Packaging: 0.1g
Price: $142
Updated: 2025/07/31

American Custom Chemicals Corporation

Product number: FRL0002126
Product name: 6-FAM AMIDITE
Purity: 95.00%
Packaging: 5MG
Price: $499.08
Updated: 2021/12/16

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5'(6)-FAM Chemical Properties,Usage,Production

Description

FAM (fluorescein) phosphoramidite 6-isomer is specifically designed for incorporation at the 5′-end of oligonucleotides, which is essential for many biochemical assays and applications. It is primarily utilized for labeling oligonucleotides, enhancing their visibility in various assays such as PCR, sequencing and hybridization studies. It is commonly employed in real-time PCR assays where fluorescent signals are monitored during amplification. The incorporation of FAM at the 5′-end allows for efficient monitoring of the reaction progress through fluorescence.

FAM emits fluorescence with a maximal emission wavelength around 517 nm, which is ideal for various detection methods. It can be quenched by agents like DusQ 1 or DABCYL, allowing for applications in assays that require precise control over fluorescence signals.

Internal sequence additions can be achieved using modified nucleotides such as FAM-dT phosphoramidite, allowing for flexible design of probes with multiple fluorescent tags while maintaining effective spacing. However, it is essential to include spacers between the labels to prevent self-quenching of fluorescence signals, which can occur due to close proximity.

The dye soluble in common organic solvents such as acetonitrile, DCM, DMF, DMSO. Once conjugated to biomolecules, the resulting FAM conjugates can be used in aqueous applications.

Chemical Properties

5'-Fluorescein phosphoramidate is soluble in common organic solvents such as acetonitrile, DCM, DMF, and DMSO. After conjugation with biomolecules, the resulting FAM conjugates can be used in aqueous applications.

Uses

6-Fluorescein phosphoramidite is a potent fluorescent dye. 6-Fluorescein phosphoramidite can be used to label oligonucleotides[1].

References

[1] Lietard J, et al. Sequence-dependent quenching of fluorescein fluorescence on single-stranded and double-stranded DNA. RSC Adv. 2022 Feb 16;12(9):5629-5637. DOI:10.1039/d2ra00534d

Abs/Em Maxima

492/517 nm

Fluoresene quantum yield

0.93

Extinction Coefficient

74000

CF260

0.22

CF280

0.17

5'(6)-FAM Preparation Products And Raw materials

Raw materials

Preparation Products

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5'(6)-FAM Suppliers

China 107 India 1 Switzerland 1 United States 7 Global 116

Commlink Technology (Tianjin)Co.,Ltd

Tel: 022-13363078-196 13363078196
Email: Commlink2024@126.com
Country: China
ProdList: 17
Advantage: 58

Changsha Hanchen Biotechnology Co., LTD

Tel: 18175992585
Email: luohuichao@hanchbio.cn
Country: China
ProdList: 294
Advantage: 58

Shanghai Xuanheng Pharmaceutical Technology Co., LTD

Tel: 18202133986
Email: wangweijun21@sohu.com
Country: China
ProdList: 473
Advantage: 58

Guangdong Prochem Biotechnology Co. , Ltd.

Tel: 4000707101
Email: research@prochembio.com
Country: China
ProdList: 133
Advantage: 58

Shanghai Annengjian Biomedical Technology Co., LTD

Tel: 021-68781987 13818305065
Email: sales@energybiopharma.com
Country: China
ProdList: 652
Advantage: 58

Hongene Biotech Corporation

Tel: 021-64757213
Fax: 86-21-64700613
Email: info@hongene.com
Country: China
ProdList: 672
Advantage: 61

Beijing Ouhe Technology Co., Ltd

Tel: 010-010-82967028 13522913783
Fax: +86-10-82967029
Email: 2355560935@qq.com
Country: China
ProdList: 12000
Advantage: 60

Wuhu Huaren Science and Technology Co., Ltd.

Tel: 0553-5842013-801 18155347026
Fax: 086-553-5843138
Email: jinhuaren@huarensh.com
Country: China
ProdList: 255
Advantage: 64

Shanghai Hanhong Scientific Co.,Ltd.

Tel: 021-54306202 13764082696
Email: info@hanhongsci.com
Country: China
ProdList: 42934
Advantage: 64

Nanjing Asian Chemical Co., Ltd.

Tel: +86 (25) 82263158
Fax: +86 (25) 82263168
Country: China
ProdList: 262
Advantage: 60

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View Lastest Price from 5'(6)-FAM manufacturers

Henan Aochuang Chemical Co.,Ltd.

Product: 6-Fluorescein phosphoramidite 204697-37-0
Price: US $0.00-0.00/kg
Min. Order: 1kg
Purity: 98%
Supply Ability: 1Ton
Release date: 2022-09-23

204697-37-0, 5'(6)-FAMRelated Search:

6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] Propanoic acid, 2,2-dimethyl-, 1,1'-[6-[10-[bis(1-methylethyl)amino]-13-cyano-1-oxo-9,11-dioxa-2-aza-10-phosphatridec-1-yl]-2',4,4',5',7,7'-hexachloro-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl] ester FLUORESCEIN DIPROPIONATE 5'(6)-FAM N-(6-HYDROXYHEXYL)-6-CARBOXAMIDOFLUORESCEIN DIPIVALATE

5'(6)-FAM

5'-(6-FAM) CEP

6-FAM-phosphoramidite

(3',6'-Dipivaloylfluoresceinyl)-6-carboxamidohexyl)-1-O-(2-cyanoethyl)-(N,N-diisopropyl)phorphoramidite

5'-Fluorescein phosphoramidite

5-Fluorescein Phosphoramidite(6-FAM)

5'-6-FAM-PhosphoraMidite

[(3′,6′-dipivaloylfluoresceinyl)- 6-carboxamido-hexyl]- 6-O-(2-cyanoethyl)- (N,N-diisopropyl)- phosphoramidite

6-CarboxyFluorescein-Aminohexyl Amidite

6-Fluorescein Phosphoramidite

Fluorescein Phosphoramidite

5'-Fluorescein CE Phosphoramidite (6-FAM)

FAM-Phosphoramidite

6-FAM phosphoramidite [5'-Fluorescein phosphoramidite] *CAS 204697-37-0*

Propanoic acid,2,2-dimethyl-,1,1'-[6-[10-[bis(1-methylethyl)amino]-13-cyano-1-oxo-9,11-dioxa-2-aza-10-phosphatridec-1-yl]-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl]ester

6-FAM phosphoramidite [5'-Fluorescein phosphoramidite

6-Fluorescein Phosphoramidite configured for ABI

FAM Phosphoramidite, 6-Isomer

6-CARBOXYFLUORESCEINAMIDE PHOSPHORAMIDITE

Propanoic acid,2,2-dimethyl-,1,1'-[6-[10-[bis(1-methylethyl)...

6-(3',6'-dipivaloylfluoresceinyl-6-carboxamido)-hexyl-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

6-Fluorescein amidite

6-((6-(((2-Cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)hexyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(2,2-dimethylpropanoate)

5'(6)-FAM (5'-Fluorescein Phosphoramidite)

6-((6-(((2-cyanoethoxy) (diisopropylamino)phosphanyl)oxy)hexyl)carbamoyl)-3-oxo-3H-spiro [isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(2,2-dimethyl propanoate)

5'-FAM Amidite

5'-Fluorescein CEP

6'-Fluorescein CE Phosphoramidite

6-FAM phosphoramidite [5'-Fluorescein phosphoramidite]

6-FAM CE Phosphoramidite

5'-Fluorescein Phosphoramidite

5'-Fluoresceinaminophosphate

5'-Fluorescein phosphoramid

6-Fluorescein Phosphoramidite (6-FAM)

204697-37-0

C46H58N3O10P

Other Fluorescent Conjugates

Fluorescent Conjugates

Fluorescent Probes, Labels, Particles and Stains

Modifiers

Sigma-Proligo

FLUOROPHORES

FAM