5'(6)-FAM
- Product Name
- 5'(6)-FAM
- CAS No.
- 204697-37-0
- Chemical Name
- 5'(6)-FAM
- Synonyms
- 5'(6)-FAM;5'-(6-FAM) CEP;5'-FAM Amidite;5'-Fluorescein CEP;FAM-Phosphoramidite;6-FAM-phosphoramidite;6-Fluorescein amidite;5'-6-FAM-PhosphoraMidite;6-FAM CE Phosphoramidite;Fluorescein Phosphoramidite
- CBNumber
- CB1726477
- Molecular Formula
- C46H58N3O10P
- Formula Weight
- 843.94
- MOL File
- 204697-37-0.mol
5'(6)-FAM Property
- Boiling point:
- 850.6±65.0 °C(Predicted)
- storage temp.
- -20°C
- solubility
- acetonitrile: soluble 0.1M, clear, colorless to faintly yellow (liquid)
- pka
- 14.56±0.20(Predicted)
- color
- solid white to off-white
- Appearance
- off-white solid
- InChIKey
- MGPYJVWEJNTXLC-UHFFFAOYSA-N
- SMILES
- C12(C3=C(C=C(OC(=O)C(C)(C)C)C=C3)OC3=C1C=CC(OC(=O)C(C)(C)C)=C3)C1=C(C=CC(C(=O)NCCCCCCOP(N(C(C)C)C(C)C)OCCC#N)=C1)C(=O)O2
Safety
- WGK Germany
- 3
N-Bromosuccinimide Price
- Product number
- M041130
- Product name
- 6-Fluorescein Phosphoramidite
- Purity
- configured for ABI
- Packaging
- 0.1g
- Price
- $135
- Updated
- 2025/07/31
- Product number
- M041130
- Product name
- 6-Fluorescein Phosphoramidite
- Purity
- configured for ABI
- Packaging
- 0.25g
- Price
- $570
- Updated
- 2025/07/31
- Product number
- M041180
- Product name
- 6-Fluorescein Phosphoramidite
- Purity
- configured for PerkinElmer, configured for Polygen
- Packaging
- 0.1g
- Price
- $142
- Updated
- 2025/07/31
- Product number
- FRL0002126
- Product name
- 6-FAM AMIDITE
- Purity
- 95.00%
- Packaging
- 5MG
- Price
- $499.08
- Updated
- 2021/12/16
5'(6)-FAM Chemical Properties,Usage,Production
Description
FAM (fluorescein) phosphoramidite 6-isomer is specifically designed for incorporation at the 5′-end of oligonucleotides, which is essential for many biochemical assays and applications. It is primarily utilized for labeling oligonucleotides, enhancing their visibility in various assays such as PCR, sequencing and hybridization studies. It is commonly employed in real-time PCR assays where fluorescent signals are monitored during amplification. The incorporation of FAM at the 5′-end allows for efficient monitoring of the reaction progress through fluorescence.
FAM emits fluorescence with a maximal emission wavelength around 517 nm, which is ideal for various detection methods. It can be quenched by agents like DusQ 1 or DABCYL, allowing for applications in assays that require precise control over fluorescence signals.
Internal sequence additions can be achieved using modified nucleotides such as FAM-dT phosphoramidite, allowing for flexible design of probes with multiple fluorescent tags while maintaining effective spacing. However, it is essential to include spacers between the labels to prevent self-quenching of fluorescence signals, which can occur due to close proximity.
The dye soluble in common organic solvents such as acetonitrile, DCM, DMF, DMSO. Once conjugated to biomolecules, the resulting FAM conjugates can be used in aqueous applications.
Chemical Properties
5'-Fluorescein phosphoramidate is soluble in common organic solvents such as acetonitrile, DCM, DMF, and DMSO. After conjugation with biomolecules, the resulting FAM conjugates can be used in aqueous applications.
Uses
6-Fluorescein phosphoramidite is a potent fluorescent dye. 6-Fluorescein phosphoramidite can be used to label oligonucleotides[1].
References
[1] Lietard J, et al. Sequence-dependent quenching of fluorescein fluorescence on single-stranded and double-stranded DNA. RSC Adv. 2022 Feb 16;12(9):5629-5637. DOI:10.1039/d2ra00534d
Abs/Em Maxima
492/517 nm
Fluoresene quantum yield
0.93
Extinction Coefficient
74000
CF260
0.22
CF280
0.17
5'(6)-FAM Preparation Products And Raw materials
Raw materials
Preparation Products
5'(6)-FAM Suppliers
- Tel
- --
- Fax
- --
- info@sapalaorganics.com
- Country
- India
- ProdList
- 1321
- Advantage
- 58
View Lastest Price from 5'(6)-FAM manufacturers
- Product
- 6-Fluorescein phosphoramidite 204697-37-0
- Price
- US $0.00-0.00/kg
- Min. Order
- 1kg
- Purity
- 98%
- Supply Ability
- 1Ton
- Release date
- 2022-09-23