Phenolphthalein diphosphate

Phenolphthalein diphosphate Property

Melting point:

198 °C

Boiling point:

762.1±70.0 °C(Predicted)

Density 

1.671±0.06 g/cm3(Predicted)

storage temp. 

−20°C

form 

solid

pka

0.88±0.30(Predicted)

Water Solubility 

NEGLIGIBLE

CAS DataBase Reference

2090-82-6

EPA Substance Registry System

1(3H)-Isobenzofuranone, 3,3-bis[4-(phosphonooxy)phenyl]- (2090-82-6)

Safety

Safety Statements 

24/25

WGK Germany 

3

HS Code 

29322985

Hazard and Precautionary Statements (GHS)

Phenolphthalein diphosphate Chemical Properties,Usage,Production

Uses

The most commonly used reagents for determining acid phosphatase are phenolphthalein diphosphate or its tetrasodium salt, thymolphthalein disodium monophosphate, and naphthylamine disodium monophosphate. These substances are classic phosphate derivatives used to determine pH values and are usually used in sodium salt form. Among these reagents, phenolphthalein diphosphate or its tetrasodium salt is more effective, easier to observe, less expensive than other reagents, and has low toxicity of the phenolphthalein released into the medium.

Chemical Properties

SLIGHTLY YELLOW FINE CRYSTALLINE POWDER

Enzyme inhibitor

This water-soluble reagent (FW = 491.26 g/mol), also known as phenolphthalein diphosphate and phenolphthalein phosphate, is an alternative substrate for phosphomonoesterases (e.g., alkaline phosphatase), as is the monophosphate. Hydrolysis of the phosphate group generates free phenolphthalein which is red in alkaline conditions. Target(s): inositol 1,4,5-trisphosphate receptors, weakly inhibited; phosphoprotein phosphatase.