ACID RED 66 Property

Melting point:

181-188 °C

Flash point:

300°(572°F)

storage temp. 

room temp

solubility 

ethanol: 1 mg/mL

form 

Powder

Colour Index 

26905

color 

Dark brown powder

Water Solubility 

Appreciable (> 10%)

BRN 

3883900

Stability:

Stable. Incompatible with strong oxidizing agents.

CAS DataBase Reference

4196-99-0

EPA Substance Registry System

C.I. Acid Red 66, disodium salt (4196-99-0)

Safety

Hazard Codes 

Xi,Xn

Risk Statements 

36/37/38-20/21/22

Safety Statements 

37/39-26-36

WGK Germany 

3

TSCA 

Yes

HS Code 

32041200

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P321Specific treatment (see … on this label).

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P362Take off contaminated clothing and wash before reuse.

ACID RED 66 Chemical Properties,Usage,Production

Chemical Properties

dark red powder

Uses

Ponceau BS has been used in Masson′s trichrome stain for the staining of collagen. It has also been used for eosinophil staining.

Definition

ChEBI: An organic sodium salt having 2-[(2-hydroxynaphthalen-1-yl)diazenyl]-5-[(4-sulfonatophenyl)diazenyl]benzene-1-sulfonate as the counterion. Used as a plasma stain in Masson's trichrome.

Preparation

3,4′-Disulfo-4-aininoazobenzene?diazo, and Naphthalen-2-ol coupling.

Biochem/physiol Actions

Ponceau BS is used in Masson′s trichrome staining procedure, along with acid fuchsin, for the labeling of collagen and muscle red.

Properties and Applications

yellow light red. Soluble in water for orange red, slightly soluble in ethanol and soluble fiber element, insoluble in other organic solvents. In the dark for the strong sulfuric acid, diluted for orange red, then have a brown precipitation. In nitric acid solution for blue, then into amber color. In water solution to join strong hydrochloric acid have red precipitate; Add thick sodium hydroxide solution have yellow brown precipitation.