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IPTG

Product Name
IPTG
CAS No.
367-93-1
Chemical Name
IPTG
Synonyms
IPTG;ISOPROPYL BETA-D-1-THIOGALACTOPYRANOSIDE;Isopropyl β-D-Thiogalactopyranoside (IPTG);1-methylethyl1-thio-beta-d-galactopyranosid;IPTG, NAO;YPD BROTH E;Isopropyl D;-beta-D-thiog;actopyranoside;IPTG ULTRA PURE
CBNumber
CB2121723
Molecular Formula
C9H18O5S
Formula Weight
238.3
MOL File
367-93-1.mol
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IPTG Property

Melting point:
105 °C
alpha 
-31 º (c=1, water)
Boiling point:
350.9°C (rough estimate)
Density 
1.3329 (rough estimate)
refractive index 
1.5060 (estimate)
storage temp. 
2-8°C
solubility 
Soluble in Water or Methanol.
form 
Crystalline Powder
pka
13.00±0.70(Predicted)
color 
White
Water Solubility 
soluble
Merck 
14,5082
BRN 
4631
Stability:
Stable. Incompatible with strong oxidizing agents.
InChIKey
BPHPUYQFMNQIOC-NXRLNHOXSA-N
CAS DataBase Reference
367-93-1(CAS DataBase Reference)
EPA Substance Registry System
Isopropyl .beta.- thiogalactoside (367-93-1)
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Safety

Hazard Codes 
Xn,Xi
Risk Statements 
19-40-66-36/37/38
Safety Statements 
23-24/25-36/37-22-36/37/39-27-26
WGK Germany 
3
10
TSCA 
Yes
HS Code 
29389090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H319Causes serious eye irritation

H351Suspected of causing cancer

Precautionary statements

P281Use personal protective equipment as required.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
IPTG-RO
Product name
Isopropyl-β-D-thiogalactoside
Packaging
1g
Price
$90.1
Updated
2024/03/01
Sigma-Aldrich
Product number
IPTG-RO
Product name
Isopropyl-β-D-thiogalactoside
Packaging
5G
Price
$330
Updated
2024/03/01
Sigma-Aldrich
Product number
5800-OP
Product name
OmniPur IPTG - CAS 367-93-1 - Calbiochem
Packaging
1g
Price
$91.3
Updated
2024/03/01
Sigma-Aldrich
Product number
I1284
Product name
Isopropyl β-D-thiogalactopyranoside solution
Purity
non-ionic
Packaging
5ml
Price
$88.3
Updated
2024/03/01
Sigma-Aldrich
Product number
5800-OP
Product name
OmniPur IPTG - CAS 367-93-1 - Calbiochem
Packaging
5G
Price
$302
Updated
2024/03/01
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IPTG Chemical Properties,Usage,Production

Description

IPTG (also known as Isopropyl-β-D-Thiogalactopyranoside) is a molecular biology reagent that functions as an inducer of galactosidase activity by binding to and inhibiting the repressor. It is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. IPTG cannot be hydrolyzed or broken down by the E. coli therefore the concentration remains constant during cell replication. IPTG is often used at a final concentration ranging from 0.5-1.0 mM. X-Gal is often used with IPTG since it provides an easy visual method to monitor whether protein expression has occurred. X-Gal as the name implies contains a galactose group that once metabolized causes a bright blue color change.

Chemical Properties

White crystalline powder

Uses

Isopropyl-β-D-thiogalactoside is used as a reagent in molecular biology. It is used as an effective β-galactosidase inducer of protein expression where the gene is under the control of the lac operator. It is used with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside to identify the non-recombinant plasmid in cloning experiments by blue-white screen methodology.

Definition

ChEBI: Isopropyl beta-D-thiogalactopyranoside is an S-glycosyl compound consisting of beta-D-1-thiogalactose having an isopropyl group attached to the anomeric sulfur.

Application

IPTG (Isopropyl-β-D-thiogalactopyranoside) is a galactose analogue not recognized by β-galactosidase. IPTG is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli. It is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal. It is commonly used in cloning procedures that require induction of β-galactosidase activity. It is also used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Preparation

a synthetic method of isopropyl-β-D-thiogalactoside. The steps are as follows:
Step 1: Dissolve β-D-galactose pentaacetate in any organic solvent of methylene chloride, chloroform or 1,2-dichloroethane, and add Lewis acid (boron trifluoride ether, (Aluminum trichloride, zinc chloride) catalyst and potassium ethylxanthate or sodium ethylxanthate are reacted to obtain tetraacetylgalactose ethylxanthate after treatment.
Step 2: Dissolve tetraacetyl galactose ethyl xanthate, 2-bromopropane and sodium carbonate in methanol or ethanol at a temperature of 20°C to 80°C and react for 4-6 hours to obtain isopropyl group after treatment Isopropyl-β-D-thiogalactoside.

General Description

Inducer for β-galactosidase, an enzyme that promotes lactose utilization. Use in conjunction with X-Gal. Molecular Biology grade.
Stock solutions:
X-Gal: 20 mg/ml in DMF
IPTG: 20 mg/ml in water
Use 4:1 (X-Gal:IPTG)
Assay: ≥99%
Dioxane: None detected
Melting point: 110-114°C
Specific rotation [a]25/D (1%, water): -34.0 to -29.0°
Intended for laboratory and manufacturing use only. Not for drug, food, or household use.

Solubility in organics

IPTG is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of IPTG in ethanol and DMSO is approximately 20 mg/ml and approximately 30 mg/ml in DMF.

storage

Store powder at -20°C away from direct sunlight. Once opened and recapped, place container in a low humidity environment at the same storage temperature. Protect from moisture and light by keeping container tightly closed.

IPTG Preparation Products And Raw materials

Raw materials

Preparation Products

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IPTG Suppliers

Nantong puyue biomedical Co., Ltd
Tel
0513-0513-0513-89158300 18014392889
Fax
0513-89158198
Email
sales@puyerbiopharma.com
Country
China
ProdList
4677
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58
Nanjing Search Biotech Co., Ltd
Tel
+86-025-81682922 +86-13515111588
Fax
025-85860962
Email
sales@searchbio.com.cn
Country
China
ProdList
135
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68
Hunan Hui Bai Shi Biotechnology Co., Ltd.
Tel
0731-85526065 13308475853
Email
ivy@hnhbsj.com
Country
China
ProdList
4554
Advantage
62
Hunan Waybel Advanced Materials Co.,Ltd
Tel
133-0845-3923 13319523173
Email
ivy@hnhbsj.com
Country
China
ProdList
457
Advantage
60
Shandong Carbon Spring New Materials Co., Ltd.
Tel
13658618215
Email
308410472@qq.com
Country
China
ProdList
55
Advantage
58
Hubei deante Chemical Technology Co., Ltd
Tel
15927262683
Email
3244356795@qq.com
Country
China
ProdList
814
Advantage
58
Hubei Zhengxingyuan Fine Chemical Co., Ltd.
Tel
027-87879189 15207106154
Fax
027-87879189
Email
3386829764@qq.com
Country
China
ProdList
309
Advantage
58
Jiangsu Boquan Biotechnology Co., Ltd.
Tel
15252131412; 15252131412
Email
5954981@qq.com
Country
China
ProdList
2944
Advantage
58
Xinxiang Yicheng Biotechnology Co.,LTD
Tel
0373-6014999 18937330588
Email
xxycswsale@163.com
Country
China
ProdList
69
Advantage
58
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
Advantage
76
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View Lastest Price from IPTG manufacturers

HEBEI SHENGSUAN CHEMICAL INDUSTRY CO.,LTD
Product
IPTG 367-93-1
Price
US $100.00-75.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
5000
Release date
2024-08-20
Hebei Zhuanglai Chemical Trading Co.,Ltd
Product
Isopropyl-Beta-D-Thiogalactopyranoside IPTG 367-93-1
Price
US $200.00-95.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
20ton
Release date
2024-06-04
WUHAN FORTUNA CHEMICAL CO., LTD
Product
Isopropyl-beta-D-thiogalactopyranoside 367-93-1
Price
US $0.00/Kg/Bag
Min. Order
1KG
Purity
98%min
Supply Ability
500KG
Release date
2021-06-07

367-93-1, IPTGRelated Search:


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