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β-Nicotinamide adenine dinucleotide

Product Name
β-Nicotinamide adenine dinucleotide
CAS No.
53-84-9
Chemical Name
β-Nicotinamide adenine dinucleotide
Synonyms
DPN;NAD;B-NAD;β-DPN;β-NAD;eotide;Nad.Dpn;beta-DPN;beta-NAD;enzopride
CBNumber
CB2286280
Molecular Formula
C21H27N7O14P2
Formula Weight
663.43
MOL File
53-84-9.mol
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β-Nicotinamide adenine dinucleotide Property

Melting point:
140-142 °C (decomp)
alpha 
D20 -31.5° (c = 1.2 in water)
storage temp. 
-20°C
solubility 
H2O: 50 mg/mL
form 
Powder
color 
White
Odor
Odorless
PH
~3.0 (50mg/mL in water)
Water Solubility 
Soluble in water at 50mg/ml
Merck 
14,6344
BRN 
3584133
Stability:
Stable. Hygroscopic. Incompatible with strong oxidizing agents.
InChIKey
BAWFJGJZGIEFAR-WWRWIPRPSA-N
EPA Substance Registry System
Adenosine 5'-(trihydrogen diphosphate), P'.fwdarw.5'-ester with 3-(aminocarbonyl)-1-.beta.-D-ribofuranosylpyridinium, inner salt (53-84-9)
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Safety

Hazard Codes 
Xn,F,Xi
Risk Statements 
36-68/20/21/22-20/21/22-40-22
Safety Statements 
36-26-36/37-24/25
WGK Germany 
3
RTECS 
UU3450000
TSCA 
Yes
HS Code 
29349990
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H371May cause damage to organs

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
NAD98-RO
Product name
NAD
Purity
Grade II, free acid
Packaging
1 g
Price
$67.6
Updated
2021/12/16
Sigma-Aldrich
Product number
43410
Product name
β-Nicotinamide adenine dinucleotide hydrate
Purity
≥95% (HPLC)
Packaging
1g
Price
$92.9
Updated
2021/12/16
Sigma-Aldrich
Product number
NAD100-RO
Product name
NAD
Purity
Grade I, free acid
Packaging
1 g
Price
$111
Updated
2021/12/16
Sigma-Aldrich
Product number
N8285
Product name
β-Nicotinamide adenine dinucleotide
Purity
pkg of 10?mg (per vial)
Packaging
15 vials
Price
$201
Updated
2021/12/16
Sigma-Aldrich
Product number
N8410
Product name
β-Nicotinamide adenine dinucleotide
Purity
pkg of 20?mg (per vial)
Packaging
15 vials
Price
$207
Updated
2021/12/16
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β-Nicotinamide adenine dinucleotide Chemical Properties,Usage,Production

Description

β-Nicotinamide adenine dinucleotide (NAD+) plays a major role in metabolism as a cofactor and mobile electron acceptor. NAD+ is a required oxidizing cosubstrate for many enzymes. It is reduced to NADH (Cat# N-035) which carries electrons to the electron transport chain for subsequent oxidative phorphorylation and ATP production. NAD+ is capable of donating ADP-ribose moieties to a protein, producing nicotinamide in the process. Sirtuin enzymes use NAD+ as a substrate to deacetylate proteins and direct activity between the nucleus and mitochondria. NAD+ is regenerated by fermentation and by oxidative phosphorylation.

Description

NAD+, known more formally as nicotinamide adenine dinucleotide, is a signaling molecule as well as a cofactor or substrate for many enzymes. It acts as an oxidizing agent, accepting electrons from other molecules while being converted to its reduced form, NADH (Item No. 16078). NAD+ is also essential for the activity of several enzymes, including poly(ADP)-ribose polymerases and cADP-ribose synthases. For example, it is used by some sirtuins to mediate protein deacetylation, producing O-acetyl-ADP-ribose and nicotinamide as well as the deacetylated protein.

Chemical Properties

Beta-NICOTINAMIDE ADENINE DINUCLEOTIDE is Hygroscopic white powder.

Uses

antidepressant, oxytocic

Uses

β-Nicotinamide Adenine Dinucleotide is a coeznyme consisting of an adenine base and a nicotinamide base connected by a pair of bridging phosphate group. β-Nicotinamide Adenine Dinucleotide acts as a c oenzyme in redox reactions, as a donor of ADP-ribose moieties in ADP-ribosylation reactions and also as a precursor of the second messenger molecule cyclic ADP-ribose. β-Nicotinamide Adenine Dinucleot ide also acts as a substrate for bacterial DNA ligases and a group of enzymes called sirtuins that use NAD+ to remove acetyl groups from proteins.

Uses

β-Nicotinamide Adenine Dinucleotide acts as a coenzyme in redox reactions, as a donor of ADP-ribose moieties in ADP-ribosylation reactions and also as a precursor of the second messenger molecule cyclic ADP-ribose. β-Nicotinamide Adenine Dinucleotide also acts as a substrate for bacterial DNA ligases and a group of enzymes called sirtuins that use NAD+ to remove acetyl groups from proteins.

Definition

The oxidized form of β-Nicotinamide Adenine Dinucleotide. It exists as an anion under normal physio-logic conditions.

Application

β-Nicotinamide adenine dinucleotide (β-NAD) has been used for the preparation of deacetylated tubulin. It has also been used for UDP-glucose-6-hydrogenase (UGDH) enzyme activity assay of orital fibroblast cell lysates.
β-Nicotinamide adenine dinucleotide (β-NAD) is a neuromodulator and an inhibitory neurotransmitter. It is an agonist for purinergic (P2Y1) receptors.
β-Nicotinamide adenine dinucleotide (NAD+) and β-Nicotinamide adenine dinucleotide, reduced (NADH) comprise a coenzyme redox pair (NAD+:NADH) involved in a wide range of enzyme catalyzed oxidation reduction reactions. In addition to its redox function, NAD+/NADH is a donor of ADP-ribose units in ADP-ribosylaton (ADP-ribosyltransferases; poly(ADP-ribose) polymerases ) reactions and a precursor of cyclic ADP-ribose (ADP-ribosyl cyclases).

General Description

β-Nicotinamide adenine dinucleotide (NAD) is a ubiquitously found electron carrier and a cofactor. NAD+ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD+ complexes, the enzyme-cofactor interactions are highly conserved.

Biological Activity

NAD+, known more formally as nicotinamide adenine dinucleotide, is a signaling molecule as well as a cofactor or substrate for many enzymes. It acts as an oxidizing agent, accepting electrons from other molecules while being converted to its reduced form, NADH. NAD+ is also essential for the activity of several enzymes, including poly(ADP)-ribose polymerases and cADP-ribose synthases. For example, it is used by some sirtuins to mediate protein deacetylation, producing O-acetyl-ADP-ribose and nicotinamide as well as the deacetylated protein.

Biochem/physiol Actions

β-Nicotinamide adenine dinucleotide (β-NAD) is an electron carrier and a cofactor, significantly involved in enzyme-catalyzed oxido-reduction processes and many genetic processes. NAD cycles between the oxidized (NAD+) and reduced (NADH) forms to maintain a redox balance necessary for continued cell growth. NAD is also involved in microbial catabolism. β-NAD acts as a substrate for various enzymes in several cellular processes.

Purification Methods

NAD is purified by paper chromatography or better on a Dowex-1 ion-exchange resin. The column is prepared by washing with 3M HCl until free of material absorbing at 260nm, then with water, 2M sodium formate until free of chloride ions and, finally, with water. NAD, as a 0.2% solution in water, is adjusted with NaOH to pH 8, and adsorbed onto the column, washed with water, and eluted with 0.1M formic acid. Fractions with strong absorption at 360nm are combined, acidified to pH 2.0 with 2M HCl, and cold acetone (ca 5L/g of NAD) is added slowly and with constant agitation. It is left overnight in the cold, then the precipitate is collected in a centrifuge, washed with pure acetone and dried under vacuum over CaCl2 and paraffin wax shavings [Kornberg Methods Enzymol 3 876 1957]. It has been purified by anion-exchange chromatography [Dalziel & Dickinson Biochemical Preparations 11 84 1966.] The purity is checked by reduction to NADH (with EtOH and yeast alcohol dehydrogenase) which has 340mn 6220 M-1cm-1. [Todd et al. J Chem Soc 3727, 3733 1957.] [pKa, Lamborg et al. J Biol Chem 231 685 1958.] The free acid crystallises from aqueous Me2CO with 3H2O and has m 140-142o. It is stable in cold neutral aqueous solutions in a desiccator (CaCl2) at 25o, but decomposes at strong acid and alkaline pH. Its purity is checked by reduction with yeast alcohol dehydrogenase and EtOH to NADH and noting the OD at 340nm. Pure NADH (see below) has 340 6.2 x 104 M-1cm-1, i.e. 0.1mole of NADH in 3mL and in a 1cm path length cell has an OD at 340nm of 0.207. [Beilstein 26 IV 3644.]

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β-Nicotinamide adenine dinucleotide Suppliers

Hubei Yuanmeng Biotechnology Co., Ltd
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027-68897569
Fax
027-68897569
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fiona@yuanmengbio.com
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China
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Hunan Hui Bai Shi Biotechnology Co., Ltd.
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0731-85526065
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Shanghai Biological Technology Development Co., Ltd.
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021-65211385-102
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021-65211385
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Shanghai Uteam Biotechnology Co., Ltd.
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Wuxi Jingyao Bio-Technology Co., Ltd.
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Nanjing UltraPure Biotech Co., Ltd.
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Syncozymes (Shanghai) Co.,Ltd.
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Hubei Wande Chemical Co., Ltd
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Hunan Yongqi Chemical Co., LTD
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Anhui Chnbio Technology Co., Ltd.
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2424422732@qq.com
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View Lastest Price from β-Nicotinamide adenine dinucleotide manufacturers

Shanxi Lianxu New Material Co., LTD
Product
beta-Diphosphopyridine nucleotide 53-84-9
Price
US $10.00/KG
Min. Order
1KG
Purity
99.9%
Supply Ability
20 tons/month
Release date
2021-10-12
Hong Kong Tiansheng New Material Trading Co., Ltd
Product
b-Diphosphopyridine Nucleotide 53-84-9
Price
US $11.90/KG
Min. Order
1KG
Purity
99%
Supply Ability
1000kg
Release date
2021-12-09
Hong Kong Tiansheng New Material Trading Co., Ltd
Product
Coenzyme I Hydrate,oxidized form 53-84-9
Price
US $58.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
1000kg
Release date
2021-12-08

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