MSP1T2

MSP1T2 Property

storage temp. 

-20°C

form 

buffered aqueous solution

biological source

microbial

Water Solubility 

water: soluble

ε(extinction coefficient)

18200M-1cm-1 at 280nm (His-tag-cleaved dissolved in 20 mM Tris pH 7.40.1M NaCl0.5mM EDTA and 0.01%NaN3)(lit.)
21000M-1cm-1 at 280nm (uncleaved His-tagged dissolved in 20 mM Tris pH 7.40.1M NaCl0.5mM EDTA and 0.01%NaN3)(lit.)

Safety

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26

WGK Germany 

WGK 3

Storage Class

11 - Combustible Solids

Hazard Classifications

Eye Irrit. 2
Skin Irrit. 2
STOT SE 3

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

MSP1T2 Chemical Properties,Usage,Production

Uses

For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

Uses

The nanodisc system has been employed to incorporate a wide variety of proteins including GPCRs, P450s, bacteriorhodopsin, coagulation factors, cholera toxin, TAR receptor and aromatase.

General Description

Nanodiscs are non-covalent structures that contain a phospholipid bilayer and a membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1). The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The Membrane Scaffold Protein 1D1 (MSP1D1) variant of MSP1 deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence.

Biochem/physiol Actions

Membrane scaffold protein 1D1 (MSP1D1) is derived from apolipoprotein A-I. It is an amphipathic synthetic protein, which self assembles to form nanodiscs.