PROTEASE INHIBITOR COCKTAIL

Product Name
PROTEASE INHIBITOR COCKTAIL
Chemical Name
PROTEASE INHIBITOR COCKTAIL
Synonyms
COMPLETE;COMPLETE, MINI;COMPLETE, EDTA-FREE;PROTEASE INHIBITOR SET;PROTEASE INHIBITOR MIX G;PROTEASE INHIBITOR MIX B;PROTEASE INHIBITOR MIX M;PROTEASE INHIBITOR MIX HP;COMPLETE, MINI, EDTA-FREE;PROTEASE INHIBITOR MIX FY
CBNumber
CB2314734
Molecular Formula
Null
Formula Weight
0
MOL File
Mol file
More
Less

PROTEASE INHIBITOR COCKTAIL Property

Flash point:
85 °C
storage temp. 
−20°C
form 
lyophilized powder
Water Solubility 
Fully miscible in water.
More
Less

Safety

Hazard Codes 
C,Xi
Risk Statements 
36/37/38-34-36/38
Safety Statements 
26-36/37/39-45-36-23-24
RIDADR 
UN 3261 8/PG 2
WGK Germany 
-
More
Less

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P337+P313IF eye irritation persists: Get medical advice/attention.

More
Less

N-Bromosuccinimide Price

Sigma-Aldrich
Product number
S8830
Product name
SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free
Purity
for use in purification of Histidine-tagged proteins
Packaging
2tablets
Price
$64.5
Updated
2024/03/01
Sigma-Aldrich
Product number
S8830
Product name
SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free
Purity
for use in purification of Histidine-tagged proteins
Packaging
20tablets
Price
$494
Updated
2024/03/01
Sigma-Aldrich
Product number
P8849
Product name
Protease Inhibitor Cocktail
Purity
for use in purification of Histidine-tagged proteins, DMSO solution
Packaging
1mL
Price
$95.8
Updated
2024/03/01
Sigma-Aldrich
Product number
P8849
Product name
Protease Inhibitor Cocktail
Purity
for use in purification of Histidine-tagged proteins, DMSO solution
Packaging
5ml
Price
$337
Updated
2024/03/01
Sigma-Aldrich
Product number
P8340
Product name
Protease Inhibitor Cocktail
Purity
for use with mammalian cell and tissue extracts, DMSO solution
Packaging
1ml
Price
$95.9
Updated
2024/03/01
More
Less

PROTEASE INHIBITOR COCKTAIL Chemical Properties,Usage,Production

Uses

Phosphatase Inhibitor Cocktail is used to inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. It has been tested on a wide variety of cells and tissue extracts to isolate/maintain intact proteins without degradation.

Enzyme inhibitor

Enzymatic proteolysis can be arrested or minimized through the use of socalled cocktails containing combinations of serine protease, cysteine protease, aspartic protease, and metalloprotease inhibitors. The following combinations (or cocktails) have proved to be highly effective in the isolation of intact proteins, and some have are so popular that they are commercially available. General Protease Cocktails typically contain AEBSF, E-64, bestatin, leupeptin, aprotinin, and sodium EDTA. The latter is included only if the protein or enzyme being purified does not require a divalent metal cofactor for structural integrity. Fungal and Yeast Protease Cocktails typically contain AEBSF, pepstatin A, E-64, and 1,10- phenanthroline. Mammalian Cell Protease Inhibitor Cocktails typically contain AEBSF, pepstatin A, E-64, bestatin, leupeptin, and aprotinin. (Other metal ion chelators, such as 0.5 mM EGTA, may be added to suppress the activity of calcium ion-dependent proteases such as calpain. Again, one must determine whether the protein or enzyme being purified does not require a divalent metal cofactor for structural intgrity or biological activity. Bacterial Extract Protease Cocktails typically contain AEBSF), pepstatin A, E-64, bestatin, and sodium EDTA. For convenience, the inhibitory properties of these reagents are summarized as follows. AEBSF: This broadspectrum serine protease inhibitor, which is stable in water for one month when stored at –20 °C, has an inhibitory range 0.1-1.0 mM. AEBSF also inhibits cysteine proteases, such as papain. Antipain: This inhibitor is effective against serine proteases (e.g., plasmin, thrombin and trypsin, but not chymotrypsin, as well as some cysteine proteases, such as calpain and papain. Its inhibitory range is 1-100 μM, with IC50 = 0.15 mg/mL for papain, 0.25 mg/mL for trypsin, 1.2 mg/mL for cathepsin A). Prepare at 10 mM in water. Aprotinin: This water-soluble inhibitor, which is stable for months, when stored at 4 °C, blocks and many serine proteases at 0.3 μM (or equimolar with proteinase), but is without effect on thrombin or bloodclotting factor Xa. Bestatin: This aminopeptidase inhibitor, which has an inhibitory range of 1-10 μM, is prepared as a 1 mM stock solution in methanol and stable for 1 month, when stored at –20 °C. Chymostatin: This chymotrypsin-like serine protease inhibitor blocks chymase and cathepsins A,B,D and G, as well as some cysteine proteases (including papain). Its inhibitory range is 10–100 μM; IC50 = 0.1 mg/mL for chymotrypsin). Stock solutions are prepared at 10 mM in DMSO. DFP: This highly effective broad-spectrum serine-protease inhibitor is also a highly toxic nerve poison, and a well-ventilated fume hood and heavy rubber gloves should be used to avoid inhalation or contact with skin. For safety, DFP should be prepared as a 200 mM solution in anhydrous isopropanol or propylene glycol and then diluted 60-100x into cell extract. In aqueous solution, DFP rapidly decomposes (t1/2 = 20 min in water). E-64: This thiol protease inhibitor, which is effective in the 1-10 μM concentration range, is prepared as a 1 mM stock solution in water and is stable 1-2 weeks at –20 °C. EDTA: This broad-spectrum metalloproteinase inhibitor (effective range = 1-10 mM) nonspecifically inhibits many metalloenzymes, especially those with metal ion Kd values above 1 mM. N-Ethylmaleimide: This chemical modifying agent inhibits most thiol-proteases by reacting irreversibly with essential active-site thiol groups. NEM is water-soluble at >10mg/ml, and must be prepared freshly. Leupeptin: This inhibitor blocks trypsin-like serine proteases, such as trypsin, chymotrypsin, chymase, pepsin and thrombin. Leupeptin inhibits selected cysteine proteases (calpain, cathepsin B, H & L and papain). The inhibitory range is 10-100 μM, with the following IC50 values: 2 mg/mL for trypsin; 8 mg/mL for plasmin; and 0.5 mg/mL for cathepsin B. When prepared at 10 mM in water, leupeptin is stable 6 months when stored at –20 °C. Pepstatin A: This inhibitor blocks aspartic proteases, such as renin, chymosin and pepsin (inhibits at 1 μM). Prepare as 1mM stock solution in methanol or DMSO. 1,10-Phenanthroline: This broad-spectrum metallo-proteinase inhibitor (with an inhibitory range of 1– 10 mM) is prepared at 200mM in methanol or DMSO. This reagent nonspecifically inhibits many metalloenzymes, especially zinc- and manganese-dependent enzymes and those with metal ion Kd values above 1 mM. PMSF: This broad-spectrum serine protease inhibitor, which is soluble in DMSO and stable for 1 month at –20 °C, has an inhibitory range of 0.1– 1.0 mM. PMSF also inhibits cysteine proteases (including papain), which are reactivated by 1 mM 2-mercaptoethanol or dithiothreitol. Phosphoramidon: This agent potently inhibits metalloendoproteinases, including thermolysin and elastases at 1–10 μM, but only weakly inhibits collagenase. When prepared at 1 mM in water, phosphoramidon solutions are stable for 1 month at –20 °C. TLCK: This haloketone-containing reagent inhibits trypsin-like serine proteases at 10-100 μM. TLCK solutions must be freshly prepared at 10 mM in 1 mM HCl and then diluted 100x into cell extracts. TPCK: This haloketone-containing reagent inhibits chymotrypsinlike serine proteases at 10-100 μM. TPCK solutions must be freshly prepared at 10 mM in 1 mM HCl and then diluted 100x into cell extracts. See also AEBSF; Antipain; Aprotinin; Bestatin; Chymostatin; E-64; NEthylmaleimide; Leupeptin; Pepstatin A; 1,10-Phenanthroline; PMSF; Phosphoramidon; TPCK

PROTEASE INHIBITOR COCKTAIL Preparation Products And Raw materials

Raw materials

Preparation Products

More
Less

PROTEASE INHIBITOR COCKTAIL Suppliers

China 48 Germany 1 Japan 2 Switzerland 1 United States 10 Global 62
Dalian Meilun Biotech Co., Ltd.
Tel
0411-62910999 13889544652
Email
sales@meilune.com
Country
China
ProdList
4727
Advantage
58
Shandong Xiya Chemical Co., Ltd.
Tel
4009903999 13395398332
Fax
0539-6365991
Email
sales@xiyashiji.com
Country
China
ProdList
20806
Advantage
60
Tianjin heowns Biochemical Technology Co., Ltd.
Tel
400-6387771-6037 18920794737
Email
sales@heowns.com
Country
China
ProdList
23016
Advantage
60
Wuhan Yanzhe Technology Co., Ltd
Tel
15527250409
Fax
QQ：2692360204
Email
wenhaotian12@163.com
Country
China
ProdList
4169
Advantage
58
JiangSu Star Science Co.,Ltd
Tel
400-400-605-7200-7200 15335295587
Email
sales037@b2star.com
Country
China
ProdList
3761
Advantage
58
Shanghai Zheyan Biotech Co., Ltd.
Tel
021-18017610038 18017610038
Email
897493074@qq.com
Country
China
ProdList
4996
Advantage
58
Shanghai Yanjin Biological Technology Co., Ltd.
Tel
--
Fax
--
Email
yanjinbio@163.com
Country
CHINA
ProdList
6896
Advantage
58
Beijing Dongge Boye Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
1861586486@qq.com
Country
CHINA
ProdList
6648
Advantage
58
Shanghai Haoran Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
470003480@QQ.COM
Country
CHINA
ProdList
6955
Advantage
58
Abcam (Shanghai) Trading Co., Ltd. (Abcam Shanghai Office)
Tel
--
Fax
--
Email
guobin@rwdmall.com
Country
CHINA
ProdList
6114
Advantage
58
Shanghai Walan Biotechnology Co., Ltd.
Tel
--
Fax
--
Country
CHINA
ProdList
4234
Advantage
58
Shanghai Yiyan Biological Technology Co., Ltd.
Tel
--
Fax
--
Email
fankesw@163.com
Country
CHINA
ProdList
3762
Advantage
58
Dongguan Biaobiao Experimental Equipment Technology Co., Ltd.
Tel
--
Fax
--
Country
CHINA
ProdList
6672
Advantage
58
Jiangxi Jianglan Pure Biological Reagent Co., Ltd.
Tel
--
Fax
--
Email
3245176082@qq.com
Country
CHINA
ProdList
6134
Advantage
58
Beijing Taize Jiaye Technology Development Co., Ltd.
Tel
--
Fax
--
Country
CHINA
ProdList
6050
Advantage
58
Shanghai Mengjia Biological Technology Co., Ltd.
Tel
--
Fax
--
Country
CHINA
ProdList
2576
Advantage
58
Beijing Bomais Technology Development Co., Ltd.
Tel
--
Fax
--
Email
15210716102@139.com
Country
CHINA
ProdList
6573
Advantage
58
Shenzhen Branch Science and Technology Co., Ltd.
Tel
--
Fax
--
Email
zikerbio@163.com
Country
CHINA
ProdList
6478
Advantage
58
Boao Pike
Tel
--
Fax
--
Email
lyn@biopike.com.cn
Country
CHINA
ProdList
6629
Advantage
58
Shanghai Weiwei Biological Technology Co., Ltd.
Tel
--
Fax
--
Email
tech@chinabiovision.com
Country
CHINA
ProdList
2731
Advantage
58
Shanghai Haoyang Biological Technology Co., Ltd.
Tel
--
Fax
--
Email
596799915@QQ.COM
Country
CHINA
ProdList
2905
Advantage
58
Beijing Xiangsheng Xingye Technology Co., Ltd.
Tel
--
Fax
--
Email
xs_bio@163.com
Country
CHINA
ProdList
5130
Advantage
58
Beijing Fubo Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
info@f-biology.com
Country
CHINA
ProdList
6025
Advantage
58
Beijing Shengke Boyuan Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
978560024@qq.com
Country
CHINA
ProdList
6005
Advantage
58
Shanghai Jingke Chemical Technology Co., Ltd.
Tel
--
Fax
--
Email
jingkehuaxue@163.com
Country
CHINA
ProdList
6972
Advantage
58
Guangzhou Weijia Technology Co., Ltd.
Tel
--
Fax
--
Email
WHIGA22@126.COM
Country
CHINA
ProdList
6748
Advantage
58
Beijing Chief-East Tech Co.,Ltd (Qwbio)
Tel
--
Fax
--
Email
info@qwbio.com
Country
China
ProdList
6912
Advantage
50
Beijing Bai Ao Si Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
yangmei@bioneeds.net
Country
CHINA
ProdList
6999
Advantage
58
Resenbio
Tel
--
Fax
--
Email
sales@xarxbio.com
Country
China
ProdList
1774
Advantage
50
Beijing Mairuibo Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
2926922538@qq.com
Country
CHINA
ProdList
1791
Advantage
58
Shanghai Minmin Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
1914109725@qq.com
Country
CHINA
ProdList
6518
Advantage
58
Beijing Zhijie Fangyuan Technology Co., Ltd.
Tel
--
Fax
--
Email
15810200961@163.com
Country
CHINA
ProdList
6984
Advantage
58
Boppard-China
Tel
--
Fax
--
Email
info@boppard.cn
Country
China
ProdList
687
Advantage
58
Beijing Baiao Innovation Technology Co., Ltd.
Tel
--
Fax
--
Email
3007606172@qq.com
Country
CHINA
ProdList
6002
Advantage
58
Selleck Chemicals
Tel
--
Fax
--
Email
info@selleck.cn
Country
China
ProdList
2542
Advantage
65
Beijing Lebo Biotechnology Co., Ltd.
Tel
--
Fax
--
Country
CHINA
ProdList
6057
Advantage
58
Shanghai Ziqi Biological Technology Co., Ltd.
Tel
--
Fax
--
Email
2453006496@qq.com
Country
CHINA
ProdList
6184
Advantage
58
Shanghai yiji industries Co., Ltd.
Tel
--
Fax
--
Email
yiji01@foxmail.com
Country
China
ProdList
6289
Advantage
50
Miaotong (Shanghai) Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
hmzhang@mt-bio.com
Country
CHINA
ProdList
6909
Advantage
58
Shanghai Machine Pure Industrial Co., Ltd.
Tel
--
Fax
--
Email
3245176082@qq.com
Country
CHINA
ProdList
6800
Advantage
58
Beijing Bi Orange Blue Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
bo49855987@163.com
Country
CHINA
ProdList
6827
Advantage
58
Beijing Taize Ruida Technology Co., Ltd.
Tel
--
Fax
--
Email
taizeorder@163.com
Country
CHINA
ProdList
6536
Advantage
58
Shanghai Yijie Biological Technology Co., Ltd.
Tel
--
Fax
--
Email
SHYIJIEBIO@126.COM
Country
CHINA
ProdList
3510
Advantage
58
Wuhan Purity Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
1546342329@qq.com
Country
CHINA
ProdList
6114
Advantage
58
Shanghai Shanran Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
shanranbio@163.com
Country
CHINA
ProdList
1563
Advantage
58
Shanghai Zeye Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
shzeysw@163.com
Country
CHINA
ProdList
6549
Advantage
58
Shanghai Yubo Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
shybio@126.com
Country
CHINA
ProdList
6967
Advantage
58
Shanghai Huzhen Industrial Co., Ltd.
Tel
--
Fax
--
Email
shhzsw01@163.com
Country
CHINA
ProdList
6478
Advantage
58

PROTEASE INHIBITOR COCKTAILRelated Search:

Bortezomib 1 VIAL PROTEASE-INHIBITOR-MIX FY PROTEASE PROTEASE

  • COMPLETE
  • COMPLETE, EDTA-FREE
  • COMPLETE, MINI
  • COMPLETE, MINI, EDTA-FREE
  • SIGMAFAST? Protease Inhibitor Cocktail Tablets, EDTA-Free
  • PROTEASE INHIBITOR SET
  • PROTEASE INHIBITOR MIX B
  • PROTEASE INHIBITOR MIX FY
  • PROTEASE INHIBITOR MIX G
  • PROTEASE INHIBITOR MIX HP
  • PROTEASE INHIBITOR MIX M
  • PROTEASE INHIBITOR COCKTAIL
  • PROTEASE INHIBITOR COCKTAIL FOR GENERAL USE
  • PROTEASE INHIBITOR COCKTAIL FOR USE IN TISSUE CULTURE MEDIA
  • PROTEASE INHIBITOR COCKTAILS
  • PROTEASE INHIBITOR COCKTAIL SET I
  • PROTEASE INHIBITOR COCKTAIL SET II
  • PROTEASE INHIBITOR COCKTAIL SET III
  • PROTEASE INHIBITOR COCKTAIL SET IV
  • PROTEASE INHIBITOR COCKTAIL SET V
  • PROTEASE INHIBITOR COCKTAIL SET VI
  • PROTEASE INHIBITOR COCKTAIL SET VII
  • Cocktail of protease inhibitors
  • Biochemicals and Reagents
  • Protease Inhibitors
  • Enzyme Inhibitors
  • Enzymes, Inhibitors, and Substrates
  • BioChemical
  • Protease Inhibitor Cocktails
  • Protease Inhibitor CocktailsProteomics
  • Cell Lysis and Protein Extraction
  • Plant Proteomics
  • Products for UpStream Processes
  • Protease Inhibitor CocktailsHIS Select Supporting Products and Reagents
  • Protease Inhibitor CocktailsPlant Biotechnology
  • Protease Inhibitor CocktailsRecombinant Protein Expression and Analysis
  • Protease InhibitorsProteomics
  • Enzyme Inhibitors
  • Native Protein Sample Preparation
  • Protease Inhibitors