ChemicalBook > CAS DataBase List > FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5

FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5

Product Name
FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5
CAS No.
273221-67-3
Chemical Name
FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5
Synonyms
Fluo 4-AM;HY-101896;Fluo4amester;Fluo-4-(acetoxymethyl) ester;Fluo-4 AM, Fluorescent labeling reagent;FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5;Fluo-4 AM, Calcium Ion Fluorescent Probe;Fluo-4 AM Calcium Fluorescent Probes (Green);Fluo-4 AM *Ultrapure Grade* *CAS 273221-67-3*;4-(6-acetoxymethoxy-2,7-difluoro-3-oxo-9-xanthenyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-n,n,n',n'-tetraacetic acid tetrakis(acetoxymethyl) ester
CBNumber
CB2497293
Molecular Formula
C51H50F2N2O23
Formula Weight
1096.94
MOL File
273221-67-3.mol
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FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 Property

Melting point:
>200℃
Boiling point:
1063.2±65.0 °C(Predicted)
Density 
1.47
storage temp. 
−20°C
solubility 
DMSO: soluble
pka
1.92±0.50(Predicted)
form 
Solid
color 
Brown to reddish brown
Appearance
orange-red powder
λmax
456nm
Biological Applications
Calcium indicator
InChIKey
QOMNQGZXFYNBNG-UHFFFAOYSA-N
SMILES
C(OCOC(C)=O)(=O)CN(C1=CC=C(C2C3=C(C=C(OCOC(C)=O)C(F)=C3)OC3C=2C=C(F)C(=O)C=3)C=C1OCCOC1=CC(C)=CC=C1N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O
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Safety

Risk Statements 
36/37/38
Safety Statements 
26-36
WGK Germany 
3
F 
10-21
HS Code 
32049010
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Tocris
Product number
6255
Product name
Fluo-4AM
Purity
≥95%(HPLC)
Packaging
1
Price
$381
Updated
2021/12/16
TRC
Product number
F647558
Product name
Fluo-4AM
Packaging
5mg
Price
$1765
Updated
2021/12/16
ChemScene
Product number
CS-7990
Product name
Fluo-4AM
Purity
≥99.0%
Packaging
100ug
Price
$150
Updated
2021/12/16
American Custom Chemicals Corporation
Product number
CHM0152903
Product name
FLUO-4-(ACETOXYMETHYL) ESTER
Purity
95.00%
Packaging
5MG
Price
$497.92
Updated
2021/12/16
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FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 Chemical Properties,Usage,Production

Description

Fluo-4 AM is a cell-permeable Ca2+-indicator that is metabolized by intracellular esterase, leading to a bright green fluorescent signal upon Ca2+-binding (excitation/emission λ at 494/506 nm). Fluo-4 AM is used for visualization and measurement of intracellular Ca2+. It is well suited for fluorometric and imaging applications such as microscopy, flow cytometry, spectrofluorometry, and fluorometric high-throughput microplate screening assays [1].


Fluo-4 AM is similar in structure and spectral properties to the widely used Ca2+-indicator, Fluo-3, but it has certain advantages over Fluo-3, such as brighter fluorescence emission, high rate of cell permeation, and a Kd for Ca2+ in buffer of 345 nM. Because of its higher fluorescence emission intensity, Fluo-4 AM can be used at lower intracellular concentrations, making its use less toxic for live cells.


As Fluo-4 AM does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. In vivo cell imaging with Fluo-4 AM is usually performed within one or two hours after loading, but the dye can be re-loaded to cells if it is needed. Fluo-4 AM can also be fixed in situ by EDC/EDAC for downstream immunofluorescence studies.


Fluo-4 AM has low solubility in the water. It is recommended to prepare 1 mM stock solution in labeling grade DMSO prior to cell loading. Use the final concentration of 1-5 μM and incubation at 37 °C for 15-60 min as a start point of your protocol.

Uses

Fluorescent Ca2+ indicator dye; cell-permeable AM ester, insensitive to magnesium.

Uses

Fluo-4 AM is a cell-permeable, fluorescent Ca2+ indicator. Displays high fluorescence emission. Excitation/emission λ 494/516 nm.

storage

Store at -20°C

Abs/Em Maxima

494/518 nm

Excitation / Emission Maximum

494 nm/506 nm

FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 Preparation Products And Raw materials

Raw materials

Preparation Products

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FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 Suppliers

CHINA 104 Switzerland 2 United Kingdom 1 United States 6 Global 113
Lingyuan Bebiansen Biological Technology Co., Ltd
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18642120371
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2837458851@qq.com
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China
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Xi'an Biolite Biotech, Inc.
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029-029-68064558 18502920169
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xiaodong@tjbiolite.com
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China
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Shanghai Annengjian Biomedical Technology Co., LTD
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021-68781987 13818305065
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sales@energybiopharma.com
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China
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Beijing Ouhe Technology Co., Ltd
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010-010-82967028 13522913783
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+86-10-82967029
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Dalian Meilun Biotech Co., Ltd.
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0411-62910999 13889544652
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sales@meilune.com
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China
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Shanghai Macklin Biochemical Co.,Ltd.
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15221275939
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021-50706099
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shenlinxing@macklin.cn
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Shanghai EFE Biological Technology Co., Ltd.
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021-65675885 18964387627
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021-65675885
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info@efebio.com
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China
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Shanghai YuanYe Biotechnology Co., Ltd.
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021-61312847; 18021002903
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QQ:3008007432
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3008007409@qq.com
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China
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71826
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Beijing Solarbio Science & Tecnology Co., Ltd.
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010-50973130 18101056239
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3193328036@qq.com
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China
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29760
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Zhengzhou Huiju Chemical Co., Ltd.
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0371-55900031 18137872243
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QQ：2853979815
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2853979815@qq.com
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China
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9975
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273221-67-3, FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5Related Search:

FLUOZIN 2 FLUOZIN 3 FLUOZIN 1 Fluo 4 Fluo 3-AM FLUO 3

  • FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5
  • Fluo 4-AM
  • 4-(6-acetoxymethoxy-2,7-difluoro-3-oxo-9-xanthenyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-n,n,n',n'-tetraacetic acid tetrakis(acetoxymethyl) ester
  • N-[4-[6-[(Acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (acetyloxy)methyl ester
  • Fluo4amester
  • Fluo-4 AM *Ultrapure Grade* *CAS 273221-67-3*
  • Fluo-4-(acetoxymethyl) ester
  • Glycine, N-[4-[6-[(acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-, (acetyloxy)methyl ester
  • 1-[2-Amino-5-(2,7-difluoro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester
  • Fluo-4 AM, Fluorescent labeling reagent
  • 1-[2-Amino-5-(2,7-difluoro-6-acetoxymethoxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, tetra(acetoxymethyl)ester
  • HY-101896
  • Fluo-4 AM Calcium Fluorescent Probes (Green)
  • Fluo-4 AM, Calcium Ion Fluorescent Probe
  • 273221-67-3
  • C51H50F2N2O23
  • Xanthene