ubiquitin cross-reactive protein Property

storage temp. 

-20°C

solubility 

Tris-HCl, pH 7.5: 1.0—1.10mg/mL, clear to slightly hazy, colorless

form 

saline suspension

biological source

human

Hazard and Precautionary Statements (GHS)

ubiquitin cross-reactive protein Chemical Properties,Usage,Production

Uses

Ubiquitin, N-terminal FLAG-tagged can replace the ubiquitin in formation of poly-ubiquitin—protein conjugates. The FLAG tag enables separation and enrichment of the protein conjugates on anti-FLAG affinity columns and detection of conjugates in western blot by anti-FLAG antibodies.

General Description

Ubiquitin, a globular protein is made of 76 amino acids and has lysine residues on its surface. It has a molecular weight of 8565 Da.

Biochem/physiol Actions

Ubiquitin interacts with the lysine residue of proteins through its ε-amino group of the C-terminal glycine residue. Proteins interacting with ubiquitin either undergo mono-ubiquitination or multi-mono-ubiquitination via a three-step process. Ubiquitination regulates intracellular trafficking and protein degradation and an imbalance in the pathway is implicated in disorders.