3,3μ-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride Property

storage temp. 

2-8°C

Safety

Hazard Codes 

F,T

Risk Statements 

23/24/25-36/38-39/23/24/25-11-45

Safety Statements 

7-16-24-33-36/37-45-53

RIDADR 

UN 3316 9/PG 2

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H225Highly Flammable liquid and vapour

H350May cause cancer

H370Causes damage to organs

Precautionary statements

P201Obtain special instructions before use.

P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P310IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.

P311Call a POISON CENTER or doctor/physician.

3,3μ-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride Chemical Properties,Usage,Production

Uses

3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride has been used as a substrate:

• in the immunoblot assay carried out to identify the changes in heat shock proteins (Hsps) expressed in the liver
• for visualization of sections after immunostaining
• in immunohistochemistry of mice knee joint sections
• in the immunolabeling of kisspeptin neurons in the preoptic area of camel

It produces a brown precipitate with peroxidase for use in immunohistological and immunoblotting staining procedures. Not recommended for ELISA (microwell) procedures.

General Description

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme and is useful as a staining agent in immunohistological and immunoblotting techniques. The end product is resistant to alcohol therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate System. The DAB Liquid Substrate System provides all the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution..