N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[2-[2-[5-[6-[(acetyloxy)methoxy]-3-oxo-3H-xanthen-9-yl]-2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]glycine (acetyloxy)methyl ester Property

Boiling point:

1051.0±65.0 °C(Predicted)

Density 

1.45±0.1 g/cm3(Predicted)

storage temp. 

-20°C

solubility 

Soluble in DMSO (up to 25 mg/ml).

form 

waxy solid

pka

1.56±0.50(Predicted)

color 

Dark

Stability:

Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 2 months.

Safety

Hazard Codes 

Xi

Risk Statements 

36/38

Safety Statements 

26

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P321Specific treatment (see … on this label).

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P362Take off contaminated clothing and wash before reuse.

N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[2-[2-[5-[6-[(acetyloxy)methoxy]-3-oxo-3H-xanthen-9-yl]-2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]glycine (acetyloxy)methyl ester Chemical Properties,Usage,Production

Description

FLUO-8 (AM) is a cell-permeable acetoxymethyl (AM) ester derivative of Fluo-8. Approximately twice as bright as Fluo-4. Absorbance 452 nm (26,000); Emission 515 nm (after AM hydrolysis; dissolved in 10 mM EGTA, 100 mM KCl, 10 mM MOPS, pH 7.2)

Uses

FLUO-8 (AM) is a green fluorescent calcium binding dye. It is a tool in making the determination of drug resistance in cells.

References

1) Minta et al. (1989), Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores; J. Biol. Chem., 264 8171