ChemicalBook > CAS DataBase List > 4,5-DIAMINOFLUORESCEIN

4,5-DIAMINOFLUORESCEIN

Product Name
4,5-DIAMINOFLUORESCEIN
CAS No.
205391-01-1
Chemical Name
4,5-DIAMINOFLUORESCEIN
Synonyms
DAF-2;5-Diaminofluorescein;4,5-DIAMINOFLUORESCEIN;5,6-DIAMINOFLUORESCEIN;DAF-2;4;5-DIAMINOFLUORESCEIN;4,5-Diaminofluorescein, DAF-2;DAF-2, Fluorescent nitric oxide probe;4,5-Diaminofluorescein-Isopropanol adduct (1:2);2-(3,6-DIHYDROXY-9H-XANTHEN-9-YL)-4,5-DIAMINO-BENZOIC ACID;5,6-Diamino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
CBNumber
CB3354894
Molecular Formula
C20H14N2O5
Formula Weight
362.34
MOL File
205391-01-1.mol
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4,5-DIAMINOFLUORESCEIN Property

storage temp. 
2-8°C
form 
powder
color 
Brown to black
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Safety

Hazard Codes 
Xn,Xi
Risk Statements 
22-36/37/38
Safety Statements 
23-26-36
WGK Germany 
3
8-10-23
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H302Harmful if swallowed

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P330Rinse mouth.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
251500-M
Product name
DAF-2-CAS205391-01-1-Calbiochem
Purity
Ahighlysensitiveandspecificfluorescentindicatorforthedirectdetectiono
Packaging
1mg
Price
$610
Updated
2025/07/31
Sigma-Aldrich
Product number
251500-M
Product name
DAF-2 - CAS 205391-01-1 - Calbiochem
Purity
A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.
Packaging
251500-1MG
Price
$591
Updated
2024/03/01
Cayman Chemical
Product number
85160
Product name
DAF-2
Purity
≥98%
Packaging
100μg
Price
$61
Updated
2024/03/01
Cayman Chemical
Product number
85160
Product name
DAF-2
Purity
≥98%
Packaging
250μg
Price
$140
Updated
2024/03/01
Sigma-Aldrich
Product number
D224
Product name
4,5-Diaminofluorescein
Purity
5 mM in DMSO, ≥97% (HPLC)
Packaging
1mg
Price
$723
Updated
2025/07/31
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4,5-DIAMINOFLUORESCEIN Chemical Properties,Usage,Production

Description

DAF-2 is a sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively. At neutral pH the detection limit for NO is 2-5 nM.

Uses

DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity.

Uses

Fluorescent detector of nitric oxide in culture medium, tissue sections and biopsy cells.

References

[1] H KOJIMA. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore.[J]. Chemical & pharmaceutical bulletin, 1998, 46 2: 373-375. DOI: 10.1248/cpb.46.373
[2] NAOKI NAKATSUBO. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins[J]. FEBS Letters, 1998, 427 2: 263-266. DOI: 10.1016/s0014-5793(98)00440-2

4,5-DIAMINOFLUORESCEIN Preparation Products And Raw materials

Raw materials

Preparation Products

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4,5-DIAMINOFLUORESCEIN Suppliers

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View Lastest Price from 4,5-DIAMINOFLUORESCEIN manufacturers

Xian confluore Biological Technology Co., Ltd.
Product
DAF-2 205391-01-1
Price
US $0.00/mg
Min. Order
5mg
Purity
>99.00%
Supply Ability
5mg
Release date
2024-03-28

205391-01-1, 4,5-DIAMINOFLUORESCEINRelated Search:


  • 2-(3,6-DIHYDROXY-9H-XANTHEN-9-YL)-4,5-DIAMINO-BENZOIC ACID
  • 4,5-DIAMINOFLUORESCEIN
  • DAF-2
  • 5,6-DIAMINOFLUORESCEIN
  • 4,5-Diaminofluorescein, DAF-2
  • 4,5-Diaminofluorescein-Isopropanol adduct (1:2)
  • 5-Diaminofluorescein
  • DAF-2;4;5-DIAMINOFLUORESCEIN
  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 5,6-diamino-3',6'-dihydroxy-
  • 5,6-Diamino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
  • DAF-2, Fluorescent nitric oxide probe
  • 205391-01-1
  • C20H14N2O5
  • C20H14N2O52C3H8O