Bromophenol Blue
- Product Name
- Bromophenol Blue
- CAS No.
- 115-39-9
- Chemical Name
- Bromophenol Blue
- Synonyms
- BPB;BROMPHENOL BLUE;BROMOPHENOL BLUE INDICATOR;ALBUTEST;NSC 7818;Aalbutest;Bromophnol blue;Bromophemol blue;BROMOPHENOL BLUE;bleudebromophenol
- CBNumber
- CB3492457
- Molecular Formula
- C19H10Br4O5S
- Formula Weight
- 669.96
- MOL File
- 115-39-9.mol
Bromophenol Blue Property
- Melting point:
- 273 °C
- Density
- 0.954 g/mL at 20 °C
- Flash point:
- 58 °C
- storage temp.
- Store at RT.
- solubility
- methanol: soluble10mg/mL
- form
- Solid
- pka
- 3.85(at 25℃)
- Boiling point:
- 605.6±55.0 °C(Predicted)
- color
- Reddish-violet
- PH Range
- 3(Yellow)-4.6(Blue) 6(yellow)-7.6(blue)
- Odor
- Odorless
- PH
- pH : 3.0～4.6
- Water Solubility
- Freely soluble in NaOH. Soluble in sodium hydroxide, alcohol, benzene, and acetic acid. Slightly soluble in water.
- λmax
- 598nm, 592nm, 436nm, 422nm, 273nm
- Merck
- 14,1444
- BRN
- 61698
- Stability:
- Stable. Incompatible with strong oxidizing agents.
- Major Application
- Nanotechnology, semiconductors, sol-gel matrix, fuel cells, display device, paints, packaging system, diapers, contact lens, determining nucleic acids, in saliva, water alkalinity, preserving genetic materials, detecting bacteria, vaginal infection test method
- InChIKey
- UDSAIICHUKSCKT-UHFFFAOYSA-N
- LogP
- 6.880 (est)
- CAS DataBase Reference
- 115-39-9(CAS DataBase Reference)
- EPA Substance Registry System
- Bromophenol blue (115-39-9)
Safety
- Hazard Codes
- T,Xn
- Risk Statements
- 61-20/21-36-36/37/38-20/21/22
- Safety Statements
- 53-45-24/25-22-36-26
- RIDADR
- UN 2265 3/PG 3
- WGK Germany
- 3
- RTECS
- SJ7453000
- TSCA
- Yes
- HazardClass
- 3
- PackingGroup
- III
- HS Code
- 29349990
- Hazardous Substances Data
- 115-39-9(Hazardous Substances Data)
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Danger
- Hazard statements
-
H226Flammable liquid and vapour
H319Causes serious eye irritation
H340May cause genetic defects
H360May damage fertility or the unborn child
H372Causes damage to organs through prolonged or repeated exposure
H373May cause damage to organs through prolonged or repeated exposure
- Precautionary statements
-
P201Obtain special instructions before use.
P202Do not handle until all safety precautions have been read and understood.
P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.
P233Keep container tightly closed.
P240Ground/bond container and receiving equipment.
P260Do not breathe dust/fume/gas/mist/vapours/spray.
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P270Do not eat, drink or smoke when using this product.
P271Use only outdoors or in a well-ventilated area.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P308+P313IF exposed or concerned: Get medical advice/attention.
P403+P233Store in a well-ventilated place. Keep container tightly closed.
P405Store locked up.
P501Dispose of contents/container to..…
N-Bromosuccinimide Price
- Product number
- 114391
- Product name
- Bromophenol Blue
- Purity
- ACS reagent
- Packaging
- 5g
- Price
- $32.1
- Updated
- 2024/03/01
- Product number
- 114391
- Product name
- Bromophenol Blue
- Purity
- ACS reagent
- Packaging
- 25g
- Price
- $103
- Updated
- 2024/03/01
- Product number
- 1.08122
- Product name
- Bromophenol blue
- Purity
- indicator ACS,Reag. Ph Eur
- Packaging
- 5G
- Price
- $157
- Updated
- 2024/03/01
- Product number
- 1.08122
- Product name
- Bromophenol blue
- Purity
- indicator ACS,Reag. Ph Eur
- Packaging
- 25g
- Price
- $392
- Updated
- 2024/03/01
- Product number
- BX1410
- Product name
- Bromophenol Blue
- Purity
- Meets ACS Specifications GR ACS
- Packaging
- 5G
- Price
- $87
- Updated
- 2022/05/15
Bromophenol Blue Chemical Properties,Usage,Production
Chemical Properties
Bromophenol Blue is pink or red powder, soluble in ethanol, benzene, ether and other organic solvents, slightly soluble in water, the aqueous solution is yellow, and blue-purple in alkaline solution. It is mainly used as an acid-base indicator, the pH discoloration range is 3.0 to 4.6, and the color changes from yellow to green to blue-violet.
Uses
Bromophenol blue is used as a tracking dye in electrophoresis, as a color marker in gel electrophoresis and polyacrylamide gel electrophoresis. As a biological stain, it plays an important role to stain proteins and nucleic acids. It acts an intermediate and an acid-base indicator in the pH range 3.0 to 4.6.
Definition
ChEBI: Bromophenol blue is 3H-2,1-Benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 3,5-dibromo-4-hydroxyphenyl groups. It is used as a laboratory indicator, changing from yellow below pH 3 to purple at pH 4.6, and as a size marker for monitoring the progress of agarose gel and polyacrylamide gel electrophoresis. It has also been used as an industrial dye. It has a role as a two-colour indicator, an acid-base indicator and a dye. It is a sultone, an arenesulfonate ester, a 2,1-benzoxathiole, a member of phenols and an organobromine compound.
Preparation
Bromophenol Blue is prepared by slow addition of excess bromine to a hot solution of phenolsulfonphthalein in glacial acetic acid.
Synthesis steps: Dissolve phenol red in glacial acetic acid, add a solution of bromine in glacial acetic acid with stirring, pour into 60°C hot water after stirring for a few minutes, cool to room temperature, and place overnight. Filter, wash the filter cake with glacial acetic acid and benzene in turn, and air dry to obtain bromophenol blue.
Biotechnological Applications
Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier.
storage
Room temperature
Purification Methods
Crystallise the dye from *C6H6 or Me2CO/AcOH, and dry it in air. It is an indicator: at pH 3.0 it is yellow and it is purple at pH 4.6. [Beilstein 19/3 V 458.]
Bromophenol Blue Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from Bromophenol Blue manufacturers
- Product
- Bromophenol Blue 115-39-9
- Price
- US $31.00-1.20/kg
- Min. Order
- 1kg
- Purity
- 99%
- Supply Ability
- g-kg-tons, free sample is available
- Release date
- 2024-03-25
- Product
- Bromophenol Blue 115-39-9
- Price
- US $0.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 50000KG/month
- Release date
- 2023-07-31
- Product
- Bromophenol blue 115-39-9
- Price
- US $233.10-190.00/kg
- Min. Order
- 1kg
- Purity
- 98%
- Supply Ability
- 1000kg
- Release date
- 2023-02-21