ChemicalBook > CAS DataBase List > Bromophenol Blue

Bromophenol Blue

Product Name
Bromophenol Blue
CAS No.
115-39-9
Chemical Name
Bromophenol Blue
Synonyms
BPB;BROMPHENOL BLUE;BROMOPHENOL BLUE INDICATOR;ALBUTEST;NSC 7818;Aalbutest;Bromophnol blue;Bromophemol blue;BROMOPHENOL BLUE;bleudebromophenol
CBNumber
CB3492457
Molecular Formula
C19H10Br4O5S
Formula Weight
669.96
MOL File
115-39-9.mol
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Bromophenol Blue Property

Melting point:
273 °C
Density 
0.954 g/mL at 20 °C
bulk density
730kg/m3
Flash point:
58 °C
storage temp. 
Store at RT.
solubility 
methanol: soluble10mg/mL
form 
Solid
pka
3.85(at 25℃)
Boiling point:
605.6±55.0 °C(Predicted)
color 
Reddish-violet
PH Range
3(Yellow)-4.6(Blue) 6(yellow)-7.6(blue)
Odor
Odorless
PH
pH : 3.0～4.6
Water Solubility 
Freely soluble in NaOH. Soluble in sodium hydroxide, alcohol, benzene, and acetic acid. Slightly soluble in water.
λmax
598nm, 592nm, 436nm, 422nm, 273nm
Merck 
14,1444
BRN 
61698
Stability:
Stable. Incompatible with strong oxidizing agents.
Major Application
Nanotechnology, semiconductors, sol-gel matrix, fuel cells, display device, paints, packaging system, diapers, contact lens, determining nucleic acids, in saliva, water alkalinity, preserving genetic materials, detecting bacteria, vaginal infection test method
InChIKey
UDSAIICHUKSCKT-UHFFFAOYSA-N
LogP
6.880 (est)
CAS DataBase Reference
115-39-9(CAS DataBase Reference)
EPA Substance Registry System
Bromophenol blue (115-39-9)
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Safety

Hazard Codes 
T,Xn
Risk Statements 
61-20/21-36-36/37/38-20/21/22
Safety Statements 
53-45-24/25-22-36-26
RIDADR 
UN 2265 3/PG 3
WGK Germany 
3
RTECS 
SJ7453000
TSCA 
Yes
HazardClass 
3
PackingGroup 
III
HS Code 
29349990
Hazardous Substances Data
115-39-9(Hazardous Substances Data)
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H226Flammable liquid and vapour

H319Causes serious eye irritation

H340May cause genetic defects

H360May damage fertility or the unborn child

H372Causes damage to organs through prolonged or repeated exposure

H373May cause damage to organs through prolonged or repeated exposure

Precautionary statements

P201Obtain special instructions before use.

P202Do not handle until all safety precautions have been read and understood.

P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.

P233Keep container tightly closed.

P240Ground/bond container and receiving equipment.

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.

P308+P313IF exposed or concerned: Get medical advice/attention.

P403+P233Store in a well-ventilated place. Keep container tightly closed.

P405Store locked up.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
114391
Product name
Bromophenol Blue
Purity
ACS reagent
Packaging
5g
Price
$32.1
Updated
2024/03/01
Sigma-Aldrich
Product number
114391
Product name
Bromophenol Blue
Purity
ACS reagent
Packaging
25g
Price
$103
Updated
2024/03/01
Sigma-Aldrich
Product number
1.08122
Product name
Bromophenol blue
Purity
indicator ACS,Reag. Ph Eur
Packaging
5G
Price
$157
Updated
2024/03/01
Sigma-Aldrich
Product number
1.08122
Product name
Bromophenol blue
Purity
indicator ACS,Reag. Ph Eur
Packaging
25g
Price
$392
Updated
2024/03/01
Sigma-Aldrich
Product number
BX1410
Product name
Bromophenol Blue
Purity
Meets ACS Specifications GR ACS
Packaging
5G
Price
$87
Updated
2022/05/15
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Bromophenol Blue Chemical Properties,Usage,Production

Chemical Properties

Bromophenol Blue is pink or red powder, soluble in ethanol, benzene, ether and other organic solvents, slightly soluble in water, the aqueous solution is yellow, and blue-purple in alkaline solution. It is mainly used as an acid-base indicator, the pH discoloration range is 3.0 to 4.6, and the color changes from yellow to green to blue-violet.

Uses

Bromophenol blue is used as a tracking dye in electrophoresis, as a color marker in gel electrophoresis and polyacrylamide gel electrophoresis. As a biological stain, it plays an important role to stain proteins and nucleic acids. It acts an intermediate and an acid-base indicator in the pH range 3.0 to 4.6.

Definition

ChEBI: Bromophenol blue is 3H-2,1-Benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 3,5-dibromo-4-hydroxyphenyl groups. It is used as a laboratory indicator, changing from yellow below pH 3 to purple at pH 4.6, and as a size marker for monitoring the progress of agarose gel and polyacrylamide gel electrophoresis. It has also been used as an industrial dye. It has a role as a two-colour indicator, an acid-base indicator and a dye. It is a sultone, an arenesulfonate ester, a 2,1-benzoxathiole, a member of phenols and an organobromine compound.

Preparation

Bromophenol Blue is prepared by slow addition of excess bromine to a hot solution of phenolsulfonphthalein in glacial acetic acid.
Synthesis steps: Dissolve phenol red in glacial acetic acid, add a solution of bromine in glacial acetic acid with stirring, pour into 60°C hot water after stirring for a few minutes, cool to room temperature, and place overnight. Filter, wash the filter cake with glacial acetic acid and benzene in turn, and air dry to obtain bromophenol blue.

Biotechnological Applications

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier.

storage

Room temperature

Purification Methods

Crystallise the dye from *C6H6 or Me2CO/AcOH, and dry it in air. It is an indicator: at pH 3.0 it is yellow and it is purple at pH 4.6. [Beilstein 19/3 V 458.]

Bromophenol Blue Preparation Products And Raw materials

Raw materials

Phenol Red Amyl acetate

Preparation Products

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View Lastest Price from Bromophenol Blue manufacturers

Hebei Zhuanglai Chemical Trading Co.,Ltd
Product
Bromophenol Blue 115-39-9
Price
US $180.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
20ton
Release date
2024-05-31
Shandong Deshang Chemical Co., Ltd.
Product
Bromophenol Blue 115-39-9
Price
US $9.80-6.60/kg
Min. Order
1kg
Purity
99.99%
Supply Ability
10 tons
Release date
2024-06-21
Hebei Chuanghai Biotechnology Co,.LTD
Product
Bromophenol Blue 115-39-9
Price
US $5.00-2.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
10000kg
Release date
2024-08-14

115-39-9, Bromophenol BlueRelated Search:

3-Bromofluorobenzene Propionyl bromide 1,2-Dibromobenzene 4-Bromobenzaldehyde 1,4-Dibromobenzene 2-Bromobenzaldehyde Phenylmethylsulfonyl fluoride Phenol Red Chlorosulfonyl isocyanate 2-Bromobenzonitrile 3,4,5,6-Tetrabromophenolsulfonephthalein Bromocresol green Trypan Blue Bromoxynil octanoate 3',3",5',5"-Tetrabromophenolsulfophthalein sodium salt BROMOPHENOL BLUE SODIUM SALT Bromophenol Red Bromonitromethane

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  • ALBUTEST
  • ALPHA,ALPHA-BIS(3,5-DIBROMO-4-HYDROXYPHENOL)-ALPHA-HYDROXY-O-TOLUENESULFONIC ACID
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  • NSC 7818
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