NUCLEAR YELLOW
- Product Name
- NUCLEAR YELLOW
- CAS No.
- 74681-68-8
- Chemical Name
- NUCLEAR YELLOW
- Synonyms
- approx.80%;Hoechst s769121;Nuclear yellow, BR;NUCLEAR YELLOW APPROX. 80%;Hoechst S769121 Trihydrochloride;Nuclear Yellow [Hoechst S769121] *CAS 74681-68-8*;4-[5-(4-methyl-1-piperazinyl)[2,50-bi-1hbenzimidazol]- 20-yl]-benzenesulfonamide;4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]-benzenesulfonamide, trihydrochloride;Benzenesulfonamide, 4-[5-(4-methyl-1- piperazinyl)[2,50-bi-1h-benzimidazol]-20-yl]-, Trihydrochloride
- CBNumber
- CB3502419
- Molecular Formula
- C25H25N7O2S.3ClH
- Formula Weight
- 596.968
- MOL File
- 74681-68-8.mol
NUCLEAR YELLOW Property
- Melting point:
- >300℃
- Density
- 1.3423 (rough estimate)
- refractive index
- 1.6000 (estimate)
- storage temp.
- Inert atmosphere,Store in freezer, under -20°C
- solubility
- Soluble in dimethyl sulfoxide, water
- form
- powder
- color
- Yellow to green
- Appearance
- light brown solid
- λmax
- 355 nm
- Biological Applications
- Detecting nucleic acid,proteins,carbohydrates,lipids; carrying out polymerase chain reaction (PCR); drug-targeting agent; nerve mapping
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H302Harmful if swallowed
H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- 25178
- Product name
- Nuclear Yellow
- Packaging
- 5mg
- Price
- $73
- Updated
- 2024/03/01
- Product number
- 25178
- Product name
- Nuclear Yellow
- Packaging
- 10mg
- Price
- $138
- Updated
- 2024/03/01
- Product number
- 25178
- Product name
- Nuclear Yellow
- Packaging
- 25mg
- Price
- $306
- Updated
- 2024/03/01
- Product number
- 8837CA
- Product name
- Nuclearyellow
- Packaging
- 25mg
- Price
- $438
- Updated
- 2021/12/16
- Product number
- DYE0000714
- Product name
- NUCLEAR YELLOW
- Purity
- 95.00%
- Packaging
- 5MG
- Price
- $495.45
- Updated
- 2021/12/16
NUCLEAR YELLOW Chemical Properties,Usage,Production
Description
Hoechst S769121 (Nuclear Yellow) is a cell-permeant yellow-emitting fluorescent dye that binds strongly to adenine-thymine-rich regions in the minor groove of double-stranded DNA. Although Nuclear Yellow can bind to all nucleic acids, AT-rich dsDNA strands enhance its fluorescence considerably.
Nuclear Yellow is used in fluorescence microscopy, fluorometry, and flow cytometry to stain and measure DNA content in live and fixed cells. It is commonly used in combination with retrograde tracers such as True Blue for two-color neuronal imaging. Nuclear Yellow can also be used to photoconvert diaminobenzidine (DAB) into an electron-dense reaction product for light and electron microscopy applications.
The commonly used dye concentration to stain bacteria or eukaryote cells is 0.1–10 μg/mL.
Uses
Hoechst S 769121 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].
References
[1] Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505. DOI:10.1177/23.7.1095650
[2] a b c "Hoechst Stains". Invitrogren (Molecular Probes).
[3] Portugal J, Waring MJ. Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 DOI:10.1016/0167-4781(88)90079-6
Abs/Em Maxima
360 (complex)/505 (complex) nm
NUCLEAR YELLOW Preparation Products And Raw materials
Raw materials
Preparation Products
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