EQUILENIN Property

Melting point:

258-259°

alpha 

D16 +87° (12.8 mg made up to 1.8 ml in dioxane)

Boiling point:

349.54°C (rough estimate)

Density 

1.1305 (rough estimate)

refractive index 

1.4800 (estimate)

Flash point:

2 °C

storage temp. 

2-8°C

solubility 

DMSO (Slightly), Methanol (Slightly, Heated)

pka

pKa 9.72 (H2O t=25.0±0.02 ) (Uncertain);9.75±0.06 (Uncertain)

form 

Solid

color 

Pale Yellow to Light Beige

optical activity

+10025 (1% in dioxane)

Water Solubility 

1.52mg/L(25 ºC)

EPA Substance Registry System

Equilenin (517-09-9)

Safety

Hazard Codes 

F,Xn

Risk Statements 

11-20/21/22-36-48-40

Safety Statements 

16-26-36-36/37

RIDADR 

UN 1648 3/PG 2

WGK Germany 

2

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H225Highly Flammable liquid and vapour

H302Harmful if swallowed

H312Harmful in contact with skin

H319Causes serious eye irritation

H332Harmful if inhaled

Precautionary statements

P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

EQUILENIN Chemical Properties,Usage,Production

Uses

Equilenin is a naturally occuring estrogenic steroidal hormone (1,2) isolated from urine of pregnant mares (3). Not found in human urine. Component of conjugated estrogenic hormones. Estrogen. This compound is a contaminant of emerging concern (CECs). Drinking water contaminant candidate list 3 (CCL 3) compound as per United States Environmental Protection Agency (EPA), environmental, and food contaminants.

Uses

Estrogenic steroidal hormone isolated from urine of pregnant mares. Occurs naturally in the d-form. Not found in human urine. Component of conjugated estrogenic hormones. Estrogen.

Definition

ChEBI: Equilenin is a 3-hydroxy steroid that is estrone which carries two double bonds at positions 6 and 8. It is found in the urine of pregnant mare's and extensively used for estrogen replacement therapy in postmenopausal women. It has a role as a mammalian metabolite and an antioxidant. It is a 3-hydroxy steroid and a 17-oxo steroid.

Purification Methods

Crystallise (+)-equilenin from EtOH (solubility is 0.63% at 18o, 2.5% at 78o), aqueous EtOH or *C6H6 (Norite) and dry it in a vacuum. It sublimes on melting and at 170-180o/0.01mm. The acetate crystallises from MeOH with m 165-167o and [ ] D +71o (c 1, CHCl3). [Bachmann et al. J Am Chem Soc 62 824 1940, Beilstein 8 III 1522, 1523, 1525, 8 IV 1420.]