Phenol Red Property

Melting point:

>300 °C

Boiling point:

>300°C

Density 

1.445 Mg m-3

refractive index 

1.5300 (estimate)

Flash point:

>300°C

storage temp. 

no restrictions.

solubility 

1 M NaOH: soluble1mg/mL

form 

Solid

pka

7.9(at 25℃)

color 

Red

PH Range

6.6(YELLOW)-8.0(RED)

PH

6.8～8.4

Odor

Odorless

Water Solubility 

0.77 g/L

λmax

557nm, 558nm, 360nm, 423nm, 433nm

Merck 

14,7247

BRN 

326470

Major Application

Display device, sol-gel matrix, photoreceptors, fuel cells, waveguides, paints, detergents, cosmetics, antiperspirant composition, diapers, screening bacteria, detecting viable cells, diagnosis of urogenital ureaplasmosis, treatment of pathogen infections, amyloid-associated diseases, glaucoma, pulmonary hypertension, asthma, chronic obstructive pulmonary disease, erectile dysfunction, Raynaud’s syndrome, heparin overdose, vulvodynia, wound healing, psychoactive drugs, evaluating dental caries activity

InChIKey

BELBBZDIHDAJOR-UHFFFAOYSA-N

LogP

3.020

CAS DataBase Reference

143-74-8(CAS DataBase Reference)

EPA Substance Registry System

Phenol Red (143-74-8)

Safety

Hazard Codes 

Xi,C

Risk Statements 

36/37/38-34

Safety Statements 

37/39-26-45-36/37/39-27

WGK Germany 

3

RTECS 

SJ7490000

TSCA 

Yes

HS Code 

29349990

Toxicity

LD50 orl-rat: >600 mg/kg CTOXAO 4(2),185,71

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

Phenol Red Chemical Properties,Usage,Production

Description

Phenol red belongs to the class of triphenylmethane dyes and is widely used in the textile industry.

Chemical Properties

dark red powder

Uses

As indicator in 0.02-0.05% alcohol solution. pH 6.8 yellow, 8.4 red.

Uses

Phenol red is used as a non absorbed marker in gravimetric method. It is a pH indicator used in cell biology laboratories and in home swimming pool test kits. It is used to check the kidney function based on the excreted phenol red by colorimetric method.

Uses

Phenol red solution acts as a pH indicator for the culture medium.

Application

Phenol red solution has been used:
As a tracer dye for microinjection of zebrafish eggs.
As a component of the mMW medium for preparation of mouse embryos.
In the preparation of versene-ethylenediamine tetraacetic acid (EDTA) solution.

Preparation

Phenol red is prepared in Dulbecco′s Phosphate Buffered Saline.

Definition

ChEBI: 3H-2,1-Benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 4-hydroxyphenyl groups. A pH indicator changing colour from yellow below pH 6.8 to bright pink above pH 8.2, it is commonly used as an indicator in cell cultures and in home swimming pool test kits. It is also used in the (now infrequently performed) phenolsulfonphthalein (PSP) test for estimation of overall blood flow through the kidney.

Biochem/physiol Actions

Phenol Red is a pH indicator which changes color from yellow to red.

Safety Profile

Moderately toxic by ingestion and intravenous routes. Mutation data reported. When heated to decomposition it emits toxicvapors of SOx.

Enzyme inhibitor

This pH-indicating dye (FW = 354.38 g/mol; CAS 143-74-8; pKa = 7.9; yellow < pH 6.8; red > pH 8.4), also known as phenolsulfonphthalein and sulfophthalein, is a diagnostic aid for evaluating renal function. A typical stock indicator solution is 0.1 g phenol red in 28.2 mL 10 mM NaOH and 221.8 mL water. Target(s): aldehyde reductase; glutaminase; gglutamyl transpeptidase; glutathione S-transferase; 4- methyleneglutaminase; phosphate uptake; thromboxane A2/prostaglandin H2 receptor; and thyroglobulin iodination.