RSL3 Property

Boiling point:

641.3±55.0 °C(Predicted)

Density 

1.368±0.06 g/cm3(Predicted)

storage temp. 

Inert atmosphere,Store in freezer, under -20°C

solubility 

Soluble in DMSO (45 mg/ml)

pka

16.09±0.60(Predicted)

form 

solid

color 

Off-white

Stability:

Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.

Safety

HS Code 

2933998090

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

RSL3 Chemical Properties,Usage,Production

Description

RSL3 (1219810-16-8) Inhibits glutathione peroxidase (GPX4) inducing ferroptosis.1,2? Effectively induces ferroptosis in glioblastoma cells.3? Everolimus in combination with RSL3 synergistically inhibits the viability of renal cell carcinoma (RCC) cells suggesting a mechanism by which RCC drug resistance may be overcome.4

in vitro

rsl3, which was a glutathione peroxidase (gpx) 4 inhibitor, had been cooperated with the smac mimetic bv6 to induce reactive oxygen species (ros)-dependent cell death in acute lymphoblastic leukemia cells. it was found that addition of the caspase inhibitor failed to rescue ros-induced cell death, suggesting that rsl3-induced cell death occured in a caspase-independent manner. moreover, the iron chelator deferoxamine inhibited rsl3/bv6-induced cell death significantly, however, it could not rescue cell death by erastin/bv6, indicating that rsl3/bv6-, but not erastin/bv6-mediated cell death depended on iron. in addition, it was shown that ros production was required for both rsl3/bv6- and erastin/bv6-induced cell death. in contrast, genetic or pharmacological inhibition of lipid peroxidation by gpx4 overexpression significantly decreased rsl3/bv6-induced cell death. of importance, inhibition of lipid peroxidation could protect from rsl3/bv6-stimulated ros production [1].

IC 50

100 nm

storage

Store at -20°C

References

Yang et al. (2014), Regulation of ferroptotic cancer cell death by GPX4; Cell 156 317 Kagan et al. (2017), Oxidized arachidonic and adrenic PEs navigate cells to ferroptosis; nAT. Chem. Biol. 13 81 Shengbiao et al. (2021), RSL3 Drives Ferroptosis through NF-kB Pathway Activation and GPX4 depletion in Glioblastoma; oXID. Med. Cell. Longev. 2021 2915019 Yangyun et al. (2022), Everolimus accelerates Erastin and RSL3-induced ferroptosis in renal cell carcinoma; Gene 809 145992