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O-GLYCOSIDASE

Product Name
O-GLYCOSIDASE
CAS No.
9032-92-2
Chemical Name
O-GLYCOSIDASE
Synonyms
O-Glycanase;EC 3.2.1.97;O-GLYCOSIDASE;O-GLYCAN PEPTIDE HYDROLASE;endo-α-n-acetylgalactosaminidase;O-GLYCOSIDASE, EXPRESSED IN E. COLI;ENDO-ALPHA-N-ACETYLGALACTOSAMINIDASE;o-glycosidase from streptococcus pneumoniae;Endo-α-N-acetylgalactosaminidase, O-Glycanase;O-Glycosidase from Streptococcus pneumoniae, Recombinant
CBNumber
CB4337902
Formula Weight
0
MOL File
Mol file
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O-GLYCOSIDASE Property

storage temp. 
2-8°C
form 
buffered aqueous solution
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Safety

WGK Germany 
3
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
G1163
Product name
O-Glycosidase from Streptococcus pneumoniae
Purity
recombinant, expressed in E. coli, buffered aqueous solution
Packaging
0.04unit
Price
$625
Updated
2024/03/01
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O-GLYCOSIDASE Chemical Properties,Usage,Production

Uses

O-Glycosidase is used to release the Gal-β(1,3)-GaINAc unit from O-glycans (glycoproteins or glycopeptides). Bindings to serine as well as to threonine are hydrolyzed. Substituents at the disaccharide (e.g., sialic acid) prevent the hydrolysis and, therefore, have to be removed chemically or enzymatically (e.g., with neuraminidase from Arthrobacter or from Vibrio cholerae).
O-Glycosidase has been used to selectively deglycosylated chondroitin sulfate proteoglycans (CSPGs).

General Description

O-Glycan-peptide hydrolase
O-Glycopeptide endo-D-galactosyl-N-acetyl-α-galactosamino hydrolase
O-Glycosidase is isolated from the culture filtrate of Diplococcus pneumoniae

Biochem/physiol Actions

Releases unsubstituted Ser- and Thr-linked β-Gal-(1→3)-α-GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent the liberation of the oligosaccharide from the protein. Pretreament with glycolytic enzymes to remove substituent saccharides from the O-glycan may be needed prior to cleavage using O-glycosidase..

O-GLYCOSIDASE Preparation Products And Raw materials

Raw materials

Preparation Products

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9032-92-2, O-GLYCOSIDASERelated Search:


  • EC 3.2.1.97
  • ENDO-ALPHA-N-ACETYLGALACTOSAMINIDASE
  • ENDO-ALPHA-N-ACETYLGALACTOSAMINIDASE, STREPTOCOCCUS PNEUMONIAE
  • O-GLYCOSIDASE
  • O-GLYCOPEPTIDE ENDO-D-GALACTOSYL-N-ACETYL-ALPHA-GALACTOSAMINOHYDROLASE
  • O-GLYCAN PEPTIDE HYDROLASE
  • O-GLYCOSIDASE, EXPRESSED IN E. COLI
  • endo-α-n-acetylgalactosaminidase
  • o-glycosidase from streptococcus pneumoniae
  • Endo-α-N-acetylgalactosaminidase, O-Glycanase
  • O-Glycanase
  • O-Glycosidase from Streptococcus pneumoniae, Recombinant
  • O-Glycosidase from Streptococcus pneumoniae recombinant, expressed in E. coli, buffered aqueous solution
  • 9032-92-2
  • Enzymes, Inhibitors, and Substrates
  • BioChemical
  • Application Index
  • Biochemicals and Reagents
  • Carbohydrate and PTM Analysis
  • Carbohydrate hydrolysis & PTM analysis