3-METHYL-2-((E)-3-[1-[3-(TRIMETHYLAMMONIO)PROPYL]-4(1H)-QUINOLINYLIDENE]-1-PROPENYL)-1,3-BENZOTHIAZOL-3-IUM DIIODIDE

3-METHYL-2-((E)-3-[1-[3-(TRIMETHYLAMMONIO)PROPYL]-4(1H)-QUINOLINYLIDENE]-1-PROPENYL)-1,3-BENZOTHIAZOL-3-IUM DIIODIDE Property

Melting point:

>250 ℃

solubility 

Soluble in dimethyl sulfoxide

form 

powder

color 

Red-brown

Appearance

blue solution

λmax

642 nm

ex/em

642/661 nm

ε(extinction coefficient)

102000 cm−1M−1

Φ(quantum yield)

0.11

Biological Applications

Nucleic acid hybridization; nucleic acid assay; detecting nucleic acids,cells,bacteria,bromodeoxyuridine incorporation,Human papilloma virus (HPV),pathogens; counting leukocytes

Hazard and Precautionary Statements (GHS)

3-METHYL-2-((E)-3-[1-[3-(TRIMETHYLAMMONIO)PROPYL]-4(1H)-QUINOLINYLIDENE]-1-PROPENYL)-1,3-BENZOTHIAZOL-3-IUM DIIODIDE Chemical Properties,Usage,Production

Description

TO-TAP-3 (Thiazole Red Monomer, also known as TO-PRO^®-3) is a far-red fluorescent carbocyanine monomeric dye. TO-TAP-3 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a significant fluorescence enhancement upon binding to dsDNA.

TO-TAP-3 has a bright fluorescence signal and low background. The dye is useful for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in microscopy. It may be used for monitoring of cell viability and dead cell detection in culture. The long-wavelength fluorescence of TO-TAP-3 is well separated from green and red fluorophores, which makes it ideal for multicolor fluorescence labeling experiments.

Chemical Properties

ex/em : 642/661 nm
Extinction coefficient (ε) : 102000 cm??1M??1
Quantum yield (Φ) : 0.11

Uses

TO-PRO-3 iodide is a highly efficient blue fluorescent dye that can stain cytoplasm as a cell tracer.

Definition

ChEBI: An unsymmetrical C3 cyanine dye having benzothiazolium-2-yl and quinolinium-4-yl substituents.

Abs/Em Maxima

643 (complex)/658 (complex) nm