CELESTINE BLUE Property

Melting point:

227-230 °C

form 

Powder

Colour Index 

51050

color 

Very dark brown to dark gray to black

Odor

Odorless

Merck 

13,1971

BRN 

3830911

InChIKey

VXAFJUDTZSMEFN-UHFFFAOYSA-N

CAS DataBase Reference

1562-90-9(CAS DataBase Reference)

Safety

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

22-24/25

WGK Germany 

3

HS Code 

32041200

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P321Specific treatment (see … on this label).

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P362Take off contaminated clothing and wash before reuse.

CELESTINE BLUE Chemical Properties,Usage,Production

Description

Coelestin Blue B 40, C.I. 51 050 [1562-90 9] is correspondingly obtained by action of 4 nitrosodiethylaniline hydrochloride with gallamide.

Chemical Properties

Black powder

Uses

Celestine Blue is a cationic dye used in cell staining. Dyes and metabolites.

Uses

To dye fabrics a navy-blue color with faint reddish tinge. As nuclear and connective tissue stain. One of the ingredients of Picro-Mallory stain: Lendrum, McFarlane, J. Pathol. Bacteriol. 50, 381 (1940).

Definition

ChEBI: Celestin blue B is an organic chloride salt composed of 1-carbamoyl-7-(diethylamino)-3,4-dihydroxyphenoxazin-5-ium and chloride ions in a 1:1 ratio. A histological dye used in solution with an iron alum mordant as a hematoxylin substitute in the H&E stain. It has a role as a fluorochrome and a histological dye. It contains a Celestin blue B(1+).

Preparation

excessive amounts of ?N,N-diethyl-4-nitrosobenzenamine or N,N-diethyl-4-(phenyldiazenyl)benzenamine?and 3,4,5-Trihydroxybenzamide reaction.

Synthesis

In an enamelled 6000 L ket tle equipped with a wooden gate stirrer, 110 kg of gallamide and 2000 L of alcohol (denatured, 94 %) are brought to a rapid boil. Over 10 h, 240 kg of nitrosodiethylaniline hydrochloride, dissolved in 1500 L of ethanol, is allowed to flow in. After addition of 21 kg of potassium carbonate, the reaction mixture is boiled for an other 1 h and allowed to cool to 20 ℃; 40 L of 20 % hydrochloric acid is added, and the mix ture is pressed into asuctionfilter withafiltering stone bottom. After stirring with 500 L of 20 % hydrochloric acid, the mixture is filtered under suction until the product is well dried, and the product is further dried at 70 ℃. Yield: 95 %. The ethanol is recovered by distillation.

Properties and Applications

red light navy blue. Soluble in water for red light purple, slightly soluble in ethanol for blue. In concentrated sulfuric acid for Daisy blue, dilution after magenta. Dye aqueous solution and sodium hydroxide as blue purple and blue precipitation.