PathScan Total IκBα Sandwich ELISA Antibody Pair
Description Reactivity Background References- Product Name
- PathScan Total IκBα Sandwich ELISA Antibody Pair
- Chemical Name
- PathScan Total IκBα Sandwich ELISA Antibody Pair
- Synonyms
- PathScan Total IκBα Sandwich ELISA Antibody Pair
- CBNumber
- CB45670518
- Formula Weight
- 0
- MOL File
- Mol file
PathScan Total IκBα Sandwich ELISA Antibody Pair Chemical Properties,Usage,Production
Description
Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IκBα Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by an IκBα Detection Antibody and anti-Rabbit IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of total IκBα protein.
Reactivity
Human;Mouse
Background
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified.
References
[1] Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
[2] Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
[3] Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
[4] Brown, K. et al. (1995) Science 267, 1485-8.
[5] Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
[6] Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
[7] Chen, Z.J. et al. (1996) Cell 84, 853-62.
[8] Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.
PathScan Total IκBα Sandwich ELISA Antibody Pair Preparation Products And Raw materials
Raw materials
Preparation Products
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