SP6 RNA POLYMERASE

Product Name
SP6 RNA POLYMERASE
Chemical Name
SP6 RNA POLYMERASE
Synonyms
SP6 RNA;SP6 RNA POLYMERASE
CBNumber
CB4778598
Formula Weight
0
MOL File
Mol file
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SP6 RNA POLYMERASE Property

storage temp. 
-20°C
form 
solution
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
RPOLSP6-RO
Product name
SP6 RNA Polymerase
Purity
from Escherichia coli BL 21/pSR3
Packaging
1000units
Price
$187
Updated
2024/03/01
Sigma-Aldrich
Product number
RPOLSP6-RO
Product name
SP6 RNA Polymerase
Purity
from Escherichia coli BL 21/pSR3
Packaging
5000units
Price
$715
Updated
2024/03/01
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SP6 RNA POLYMERASE Chemical Properties,Usage,Production

Uses

SP6 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from an SP6 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including:

  • RNA or DNA blotting techniques
  • In situ hybridization
  • RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
  • Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.
  • The synthesis of antisense RNA probes

General Description

With this system, homogeneously labeled single-stranded RNA can be generated. Transcripts can be non-radioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-32P] or [α-35S] labeled nucleotides.

Contents:

  • SP6 RNA Polymerase, ≥20 U/μl, in buffer, pH 7.9
  • Transcription Buffer, 10x concentrated

SP6 RNA POLYMERASE Preparation Products And Raw materials

Raw materials

Preparation Products

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SP6 RNA POLYMERASE Suppliers

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  • SP6 RNA POLYMERASE
  • SP6 RNA