ChemicalBook > CAS DataBase List > BCECF-AM

BCECF-AM

Product Name: BCECF-AM
CAS No.: 117464-70-7
Chemical Name: BCECF-AM
Synonyms: BCECF-AM;BCECF--1M;BCECF--1M 1MG;Bcecf-AmSolution;bcecf-acetoxymethyl;BCECF-Acetoxymethyl ester;BCECF AM pH Indicator (Green);BCECF/AM - CAS 117464-70-7 - Calbiochem;BCECF.AM ESTER, 1 MG/ML IN*ANHYDROUS DMSO;2,7-BIS(2-CARBOXYETHYL)-5,6-CARBOXYFLUORESCEIN
CBNumber: CB5111514
Molecular Formula: C42H40O21
Formula Weight: 880.75
MOL File: 117464-70-7.mol

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BCECF-AM Property

Boiling point:: 692.41°C (rough estimate)
Density: 1.2837 (rough estimate)
refractive index: 1.6500 (estimate)
Flash point:: 85 °C
storage temp.: -20°C
solubility: acetonitrile: 25mg/mL
DMF: soluble
DMSO: soluble
form: White to off-white solid
color: Orange to red
Appearance: white powder
CAS DataBase Reference: 117464-70-7(CAS DataBase Reference)

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Safety

Hazard Codes: Xi
Risk Statements: 36/37/38
Safety Statements: 22-26-36
WGK Germany: 3

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P321Specific treatment (see … on this label).

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P362Take off contaminated clothing and wash before reuse.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: B8806
Product name: 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester
Purity: 1 mg/mL in DMSO
Packaging: 1 mg
Price: $296
Updated: 2025/07/31

Sigma-Aldrich

Product number: 216254
Product name: BCECF/AM
Packaging: 1mg
Price: $199
Updated: 2025/07/31

Cayman Chemical

Product number: 15922
Product name: BCECF-Acetoxymethyl ester
Purity: ≥95%
Packaging: 1mg
Price: $117
Updated: 2024/03/01

Cayman Chemical

Product number: 15922
Product name: BCECF-Acetoxymethyl ester
Purity: ≥95%
Packaging: 5mg
Price: $521
Updated: 2024/03/01

Cayman Chemical

Product number: 15922
Product name: BCECF-Acetoxymethyl ester
Purity: ≥95%
Packaging: 25mg
Price: $1540
Updated: 2021/12/16

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BCECF-AM Chemical Properties,Usage,Production

Description

BCECF AM ester is a widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF AM can also be used to investigate intracellular changes in other ions, including potassium.

Chemical Properties

pale yellow to yellow powder

Uses

Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.

General Description

Material is an isomeric mixture of lactones, acetates, and AM esters

Biological Activity

Non-charged, non-fluorescent pH indicator. Readily and rapidly diffuses through cell membranes.

References

[1] T J RINK T P R Y Tsien. Cytoplasmic pH and free Mg2+ in lymphocytes.[J]. Journal of Cell Biology, 1982, 95 1: 189-196. DOI: 10.1083/jcb.95.1.189
[2] PINAR OZKAN Raj M. A rapid method for measuring intracellular pH using BCECF-AM[J]. Biochimica et biophysica acta. General subjects, 2002, 1572 1: Pages 143-148. DOI: 10.1016/s0304-4165(02)00303-3
[3] CAROLINE DIVE. Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterases[J]. Molecular and Cellular Probes, 1988, 2 2: Pages 131-145. DOI: 10.1016/0890-8508(88)90035-7
[4] NATHAN O’CONNOR Randi B S. Ratio imaging: practical considerations for measuring intracellular Ca2+ and pH in living cells.[J]. Methods in cell biology, 2013, 114: 387-406. DOI: 10.1016/b978-0-12-407761-4.00016-6
[5] SUE CHOW Ian T David Hedley. Flow cytometric calibration of intracellular pH measurements in viable cells using mixtures of weak acids and bases[J]. Cytometry Part B: Clinical Cytometry, 1996, 24 4: 360-367. DOI: 10.1002/(sici)1097-0320(19960801)24:4<360::aid-cyto7>3.0.co;2-j
[6] LáSZLó BALKAY. Flow cytometric determination of intracellular free potassium concentration[J]. Cytometry Part B: Clinical Cytometry, 1998, 28 1: 42-49. DOI: 10.1002/(sici)1097-0320(19970501)28:1<42::aid-cyto5>3.0.co;2-j

Abs/Em Maxima

440; 490/530 nm

Excitation / Emission Maximum

440-490 nm/535 nm

BCECF-AM Preparation Products And Raw materials

Raw materials

Preparation Products

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BCECF-AM Suppliers

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117464-70-7, BCECF-AMRelated Search:

4-Ethylbenzoic acid Anisyl acetate o-Cresolphthalein 4-Methylbenzhydrol FLUORESCIN 3,4-Dimethylbenzoic acid Methyl 3,4-dimethylbenzoate 5-CARBOXYFLUORESCEIN DIACETATE Dimethyl isophthalate Mono-methyl isophthalate Isophthalic acid 3-(4-Methoxyphenyl)propionic acid 3-METHYLBENZYL ACETATE 3-(2-HYDROXYPHENYL)PROPIONIC ACID Ethyl 3-methylbenzoate 4-Tolyl ether 4-Isopropylbenzoic acid 3-(4-METHOXYPHENYL)-1-PROPANOL

2,7-BIS(2-CARBOXYETHYL)-5,6-CARBOXYFLUORESCEIN

2',7'-BIS(2-CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN ACETOXYMETHYL ESTER

2',7'-BIS-(CARBOXYETHYL)-5(6')-CARBOXYFLUORESCEIN ACETOXYMETHYL ESTER

2',7'-bis(2-carboxyethyl)-5(6)-*carboxyfluorescei

BCECF.AM ESTER, 1 MG/ML IN*ANHYDROUS DMSO

Bcecf-AmSolution

2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester mixed isomers

bcecf-acetoxymethyl

2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein AM ester

BCECF-AM solution, Spiro(isobenzofuran-1(3H),9μ-(9H)xanthene)-2μ,7μ-dipropanoic acid

2',7'-Bis(2-acetoxymethoxycarbonylethyl)-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid acetoxymethyl ester

2,7-Bis[2-(acetoxymethoxycarbonyl)ethyl]-3,6-dihydroxy-3'-oxospiro[9H-xanthene-9,1'(3'H)-isobenzofuran]-6'-carboxylic acid acetoxymethyl ester

2',7'-Biscarboxyethyl-6-carboxyfluorescein acetoxymethyl ester

BCECF-AM

3',6'-bis(Acetyloxy)-5(or6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoicacid2',7'-bis[(acetyloxy)methyl]ester

BCECF/AM - CAS 117464-70-7 - Calbiochem

BCECF-Acetoxymethyl ester

BCECF--1M

BCECF--1M 1MG

SPIRO(ISOBENZOFURAN-1(3H),9'-(9H)XANTHENE)-2',7'-DIPROPANOIC ACID

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxyMet

2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethy

2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester

BCECF-AM, intracellular ratiometric pH indicator

BCECF AM pH Indicator (Green)

117464-70-7

C42H40O21

C39H36O19

Cell Signaling and Neuroscience

BioChemical

Cell Biology

Ion Probes

Ion Channels

Monovalent Ion Channels

Voltage-gated Ion Channels