4-Chloro-7-nitrobenzofurazan

Product Name: 4-Chloro-7-nitrobenzofurazan
CAS No.: 10199-89-0
Chemical Name: 4-Chloro-7-nitrobenzofurazan
Synonyms: NBD-CL;4-chloro-7-nitrobenzo[c][1,2,5]oxadiazole;4-Chloro-7-nitro-1,2,3-benzoxadiazole;4-CHLORO-7-NITROBENZOFURAZAN;NBD CHLORIDE;4-chloro-7-nitrobenzo-2,1,3-oxadiazole;7-CHLORO-4-NITROBENZO-2-OXA-1,3-DIAZOLE;4-CHLORO-7-NITROBENZOFURAZAN, FOR FLUORE SCENCE;NBC-1;nbd-c1
CBNumber: CB5173637
Molecular Formula: C6H2ClN3O3
Formula Weight: 199.55
MOL File: 10199-89-0.mol

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4-Chloro-7-nitrobenzofurazan Property

Melting point:: 97-99 °C(lit.)
Boiling point:: 333.1±45.0 °C(Predicted)
Density: 2.0589 (rough estimate)
refractive index: 1.5810 (estimate)
storage temp.: Inert atmosphere,Room Temperature
solubility: Soluble in methanol, dimethylsulfoxide, dimethylformamide and chloroform.
pka: -5.65±0.50(Predicted)
form: Crystalline Powder
color: Yellow to light brown
Water Solubility: soluble
BRN: 614212
CAS DataBase Reference: 10199-89-0(CAS DataBase Reference)
EPA Substance Registry System: 2,1,3-Benzoxadiazole, 4-chloro-7-nitro- (10199-89-0)

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Safety

Hazard Codes: Xn,Xi
Risk Statements: 22-36/38
Safety Statements: 36/37/39
RIDADR: UN 2811 6.1/PG 3
WGK Germany: 3
RTECS: DF8002400
F: 10-21
TSCA: Yes
HS Code: 29349990

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 163260
Product name: 4-Chloro-7-nitrobenzofurazan
Purity: 98%
Packaging: 1g
Price: $51
Updated: 2024/03/01

Sigma-Aldrich

Product number: 163260
Product name: 4-Chloro-7-nitrobenzofurazan
Purity: 98%
Packaging: 5g
Price: $71.3
Updated: 2024/03/01

TCI Chemical

Product number: A5592
Product name: NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)[for HPLC Labeling]
Purity: >98.0%(T)
Packaging: 1g
Price: $42
Updated: 2024/03/01

TCI Chemical

Product number: A5592
Product name: NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)[for HPLC Labeling]
Purity: >98.0%(T)
Packaging: 5g
Price: $121
Updated: 2024/03/01

Alfa Aesar

Product number: A14165
Product name: 4-Chloro-7-nitrobenzofurazan, 99%
Packaging: 5g
Price: $65.2
Updated: 2024/03/01

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4-Chloro-7-nitrobenzofurazan Chemical Properties,Usage,Production

Chemical Properties

yellow to light brown crystalline powder

Uses

4-Chloro-7-nitrobenzofurazan is used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines. It is utilized in the preparation of fluorescent phospholipid-derivative, hydroxynaphthofurazan and 4-chloro-7-nitrobenzofurazan- didecanoylphosphatidylethanolamine. It serves as a fluorescent reagent to label free sulfhydryls and N-terminals within proteins and trapping agent for cysteine sulfenic acid. It is a sensitive chromogenic and fluorogenic reagent. It reacts with trans-1-methoxy-3-(trimethylsilyloxy)-1,3-butadiene (Danishefsky's diene) to prepare regioselectively the silyl enol ether. Further, it is used to label peptides, proteins and other biomolecules. In addition it is used in the synthesis of fluorescent phospholipid-derivative, NBD-didecanoylphosphatidylethanolamine, functionalized hydroxynaphthofurazan and 7-nitrobenzofurazan (NBD)-labeled maleimide.

Uses

4-Chloro-7-nitrobenzofurazan is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. 4-Chloro-7-nitrobenzofurazan has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.

Definition

ChEBI: A benzoxadiazole that is 2,1,3-benzoxadiazole which is substituted at position 4 by chlorine and at position 7 by a nitro group.

General Description

4-Chloro-7-nitrobenzofurazan (NBD-Cl) is a highly sensitive chromogenic and fluorogenic reagent. It is reported to react spontaneously with trans-1-methoxy-3-(trimethylsilyloxy)-1,3-butadiene (Danishefsky′s diene), to afford regioselectively the silyl enol ether, via normal electron-demand Diels-Alder (NEDDA) reaction. A study of the molecular structure, vibrational spectra and NBO (Natural Bond Orbital) analysis of NBD–Cl has been undertaken. Its utility as a pre-column derivatization agent has been examined.

in vitro

4-Chloro-7-nitrobenzofurazan(NBD chloride) forms highly fluorescent derivatives for detection of all the protein amino acids. In addition, NBD-Cl provides a simple and sensitive method for determination of N-terminal amino acids. The differences in intensity and color of fluorescence can be used to advantage to identify prolyl peptides.

Purification Methods

Wash the solid with H2O, and it recrystallises from aqueous EtOH (1:1) as pale yellow needles. It sublimes in a vacuum [Gosh & Whitehouse Biochem J 108 155 1968, UV, NMR: Bolton et al. J Chem Soc 1004 1966].

4-Chloro-7-nitrobenzofurazan Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from 4-Chloro-7-nitrobenzofurazan manufacturers

R&D Scientific Inc.

Product: 4-Chloro-7-nitro-1,2,3-benzoxadiazole 10199-89-0
Price: US $1150.00/g
Min. Order: 1g
Purity: 98
Supply Ability: 500 Kg
Release date: 2024-05-30

Hebei Weibang Biotechnology Co., Ltd

Product: 4-Chloro-7-nitrobenzofurazan 10199-89-0
Price: US $10.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 10 mt
Release date: 2023-01-10

Hebei Mojin Biotechnology Co., Ltd

Product: 4-Chloro-7-nitrobenzofurazan 10199-89-0
Price: US $0.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 50000KG/month
Release date: 2023-09-06

10199-89-0, 4-Chloro-7-nitrobenzofurazanRelated Search:

4-Chloro-7-nitrobenzofurazan Nitrobenzene BENZOFURAZAN N-BENZYL-4-NITRO-2,1,3-BENZOXADIAZOL-5-AMINE 7-CHLORO-4-NITRO-5-PYRROLIDIN-1-YL-BENZO[1,2,5]OXADIAZOLE 7-CHLORO-4-NITRO-5-PIPERIDINO-2,1,3-BENZOXADIAZOLE 5-AMINO-7-CHLORO-4-NITROBENZOFURAZAN 7-CHLORO-N,N-DIETHYL-4-NITRO-2,1,3-BENZOXADIAZOL-5-AMINE 2-(7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YLAMINO)-ETHANOL 3-(7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YLAMINO)-PROPAN-1-OL AKOS BB-7022 7-CHLORO-5-(4-METHYL-PIPERAZIN-1-YL)-4-NITRO-BENZO[1,2,5]OXADIAZOLE 7-CHLORO-5-MORPHOLIN-4-YL-4-NITRO-BENZO[1,2,5]OXADIAZOLE (7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YL)-PYRIDIN-2-YL-AMINE N-[4-(7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YLAMINO)-PHENYL]-ACETAMIDE TIMTEC-BB SBB005650 (7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YL)-DIMETHYL-AMINE 4-CHLORO-5,7-DINITROBENZOFURAZAN

4-CHLORO-7-NITROBENZOFURAZAN, FOR FLUORE SCENCE

7-CHLORO-4-NITROBENZ-2-OXA-1-3-DIAZOLECR YSTALLINE

4-Chloro-7-nitrobenzo-2,1,3-oxadiazole~NBDchloride

2,1,3-Benzoxadiazole, 4-chloro-7-nitro-

4-chloro-7-nitrobenzo-2,1,3-oxadiazole

NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]

4-chloro-7-nitrobenzofurazane

4-Chloro-7-nitro-1,2,3-benzoxadiazole, NBD-chloride

NBC-1

NBD chloride,98%

NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)

4-Chloro-7-nitro-1,2

4-chloro-7-nitrobenzo[c][1,2,5]oxadiazole

7-Chloro-4-nitro-2,1,3-benzoxadiazole

NSC 228140

4-Chloro-7-nitrobenzofurazan 4-Chloro-7-nitro-2,1,3-benzoxadiazole

4-Chloro-7-nitro-2,1,3-benzoxadiazole 99%

4-chloro-7-nitro-benzofuraza

4-nitro-7-chlorobenzofurazan

nbd-c1

7-CHLORO-4-NITRO-2,1,3-BENZOXADIAZOL

7-CHLORO-4-NITROBENZO-2-OXA-1,3-DIAZOLE

7-CHLORO-4-NITROBENZOFURAZAN

AKOS BC-0686

4-CHLORO-7-NITRO-2,1,3-BENZOXADIAZOLE

4-CHLORO-7-NITROBENZ-2-OXA-1,3-DIAZOLE

4-CHLORO-7-NITRO-BENZO[1,2,5]OXADIAZOLE

4-CHLORO-7-NITROBENZO-2-OXA-1,3-DIAZOLE

4-CHLORO-7-NITROBENZOFURAZAN

4-CHLORO-7-NITROBENZUFURAZAN

4-Chloro-7-nitro-1,2,3-benzoxadiazole (NBD-Cl )

4-Chloro-7-nitrobenzo-2-furazan, NBF-Cl

1-Chloro-4-nitrobenzoxadiazole

LABOTEST-BB LT00238516

NBD CHLORIDE

NBD-CL

TIMTEC-BB SBB005345

4-Chloro-7-nitro-1,2,3-benzoxadiazole

NBD-Cl [4-Chloro-7-nitrobenzofurazan] *CAS 10199-89-0*

7-CHLORO-4-NITROBENZ-2-OXA-1,3-DIAZOLE

Benzofurazan, 4-chloro-7-nitro-

Shokat-update-comp17

NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)[for HPLC Labeling]&gt

NBD-Cl [4-Chloro-7-nitrobenzofurazan

oro-7-nitrobenzo-2-oxa-1,3-diazoL

4-Chloro-7-nitro-2,1,3-benzoxadiazole(NBD-Cl)

4-Choro-7-nitrobenzofurazan

4-Chloro-7-nitrobenzo-2-furazan

4-Chloro-7-nitrobenzo-2-oxa-1,3-oxadiazole

10199-89-0

C6H2ClN3O3

Mercapto Group Labeling Reagents for Fluorescence HPLC

HPLC Labeling Reagents

Fluorescence Detection (HPLC Labeling Reagents)

Amino Group Labeling Reagents for Fluorescence HPLC

Functional Group Reactive Labels

Functional Group Reactive Labels for Immunoassays

Fluorescent Probes, Labels, Particles and Stains