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Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)*

Product Name
Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)*
CAS No.
136632-31-0
Chemical Name
Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)*
Synonyms
Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)*;Biotin-16-dUTP,5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′-deoxyuridine5′-triphosphate
CBNumber
CB53382636
Formula Weight
0
MOL File
Mol file
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Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* Property

storage temp. 
-20°C
form 
solution
color 
colorless
Water Solubility 
water: miscible
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
11093070910
Product name
Biotin-16-dUTP
Packaging
50μL
Price
$520
Updated
2025/07/31
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Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* Chemical Properties,Usage,Production

Uses

For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation.

  • Biotin-16-dUTP is used as asubstrate for: Terminal Transferase
  • DNA polymerase I (holoenzyme and Klenow fragment)
  • Taq DNA polymerase
  • Reverse transcriptase (e.g., Transcriptor)
Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR.
The nucleotide also serves as a substrate for Terminal Transferase in 3′-end labeling.
  • Biotin-labeled DNA can be detected with: Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrate
  • Biotin Luminescence Detection Kit

General Description

Biotin is a water-soluble vitamin which functions as a coenzyme for carboxylases.

Biochem/physiol Actions

Biotin influences gene regulation and chromatin structure by associating with lysine residues in histones. It influences the actions of adaptive immune T and natural killer cells. Deficiency of biotin in mice has indicated enhanced inflammation. Studies have also indicated heightened inflammatory response of human dendritic cells in conditions of biotin deficiency.

Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* Preparation Products And Raw materials

Raw materials

Preparation Products

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Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* Suppliers

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  • Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)*
  • Biotin-16-dUTP,5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′-deoxyuridine5′-triphosphate
  • 136632-31-0