BCIP/NBT
- Product Name
- BCIP/NBT
- Chemical Name
- BCIP/NBT
- Synonyms
- NBT/BCIP;BCIP/NBT;BCIP/INT;BM PURPLE;BCIP/NBT PLUS;BCIP/NBT-BLUE;NBT-X-PHOSPHATE;SIGMAFAST? BCIP?/NBT;NBT-BCIP(R) solution;NBT/BCIP SUBSTRATE SOLN
- CBNumber
- CB5448175
- Molecular Formula
- C48H34BrClN11O10P
- Formula Weight
- 1071.18
- MOL File
- Mol file
BCIP/NBT Property
- Flash point:
- 67 °C
- storage temp.
- -20°C
- form
- tablet
- color
- dark purple
- Water Solubility
- water: 10mL, clear, yellow
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- B1911
- Product name
- BCIP<SUP>®</SUP>/NBT Liquid Substrate System
- Purity
- ready to use solution
- Packaging
- 100 mL
- Price
- $111
- Updated
- 2025/07/31
- Product number
- B1911
- Product name
- BCIP®/NBT Liquid Substrate System
- Purity
- ready to use solution
- Packaging
- 100mL
- Price
- $108
- Updated
- 2024/03/01
- Product number
- B5655
- Product name
- SIGMAFAST™ BCIP®/NBT
- Purity
- tablet
- Packaging
- 5TABLETS
- Price
- $58.3
- Updated
- 2023/06/20
- Product number
- B5655
- Product name
- SIGMAFAST™ BCIP®/NBT
- Purity
- tablet
- Packaging
- 25TABLETS
- Price
- $204
- Updated
- 2023/06/20
- Product number
- HCH0127002
- Product name
- 2-(4-IODOPHENYL)-5-(4-NITROPHENYL)-3-PHENYLTETRAZOLIUM CHLORIDE AND 5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE, TOLUIDINE-SALT
- Purity
- 95.00%
- Packaging
- 5MG
- Price
- $499.85
- Updated
- 2021/12/16
BCIP/NBT Chemical Properties,Usage,Production
Uses
SIGMAFAST BCIP?/NBT has been used as a substrate
- for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting
- for alkaline phosphatase in the histochemical staining of primary osteoblasts
- for alkaline phosphatase staining in bone marrow stromal cells
Uses
A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
General Description
BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.
Appearance prior to use: light yellow color;
After color development: dark purple
BCIP/NBT Preparation Products And Raw materials
Raw materials
Preparation Products
BCIP/NBT Suppliers
- Tel
- --
- Fax
- --
- jaeger@biotrend.com
- Country
- Germany
- ProdList
- 6115
- Advantage
- 76